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Wide-range ELISA Kit for Gelsolin (GSN)
GS; AGEL; ADF; Brevin; Gelsolin Amyloidosis,Finnish Type; Actin-depolymerizing factor
- Product No.WEA372Hu
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Sample Typeserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length3h
- Detection Range6.25-400ng/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 2.77ng/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
- FOB
US$ 372
For more details, please contact local distributors! US$ 532 US$ 2394 US$ 4522 US$ 37240
Specificity
This assay has high sensitivity and excellent specificity for detection of Wide-range Gelsolin (GSN).
No significant cross-reactivity or interference between Wide-range Gelsolin (GSN) and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Wide-range Gelsolin (GSN) and the recovery rates were calculated by comparing the measured value to the expected amount of Wide-range Gelsolin (GSN) in samples.
Matrix | Recovery range (%) | Average(%) |
serum(n=5) | 94-102 | 98 |
EDTA plasma(n=5) | 89-96 | 93 |
heparin plasma(n=5) | 88-102 | 92 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Wide-range Gelsolin (GSN) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Wide-range Gelsolin (GSN) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Wide-range Gelsolin (GSN) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample | 1:2 | 1:4 | 1:8 | 1:16 |
serum(n=5) | 80-93% | 94-103% | 93-104% | 81-98% |
EDTA plasma(n=5) | 89-96% | 81-96% | 81-104% | 94-101% |
heparin plasma(n=5) | 92-103% | 81-101% | 97-104% | 92-101% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
Reagents | Quantity | Reagents | Quantity |
Pre-coated, ready to use 96-well strip plate | 1 | Plate sealer for 96 wells | 4 |
Standard | 2 | Standard Diluent | 1×20mL |
Detection Reagent A | 1×120µL | Assay Diluent A | 1×12mL |
Detection Reagent B | 1×120µL | Assay Diluent B | 1×12mL |
TMB Substrate | 1×9mL | Stop Solution | 1×6mL |
Wash Buffer (30 × concentrate) | 1×20mL | Instruction manual | 1 |
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
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Magazine | Citations |
Neurochmical Research | Gelsolin in Cerebrospinal Fluid as a Potential Biomarker of Epilepsy SpringerLink: 3585r8h46q7r0171 |
Journal of Neuroinflammation | Treatment with gelsolin reduces brain inflammation and apoptotic signaling in mice following thermal injury BioMed: 17422094 |
Biomedical Engineering and Informatics | Application of iTRAQ quantitative proteomics in identification of serum biomarkers in breast cancer IEEE: 6098563 |
European Journal of Obstetrics & Gynecology and Reproductive Biology | Investigation of the actin scavenging system in pre-eclampsia Pubmed: 24239294 |
The Annals of Thoracic Surgery | Proteomic Study Reveals Plasma Protein Changes in Congenital Heart Diseases Pubmed: 24565402 |
BMC Anesthesiol.? | The role of plasma gelsolin in cardiopulmonary bypass induced acute lung injury in infants and young children: a pilot study Pubmed:Pmc4132929 |
Cell Biochemistry and Biophysics | Gelsolin: Role of a Functional Protein in Mitigating Radiation Injury Pubmed:25164111 |
Neurosci Lett | Plasma gelsolin and matrix metalloproteinase 3 as potential biomarkers for Alzheimer disease PubMed: 25864780 |
Proteomics Clinical Applications | Comparative mass spectrometric and immunoassay‐based proteome analysis in serum of Duchenne muscular dystrophy patients Pubmed:26680509 |
PROTEOMICS - Clinical Applications | Comparative mass spectrometric and immunoassay-based proteome analysis in serum ofDuchenne muscular dystrophy patients. pubmed:26680509 |
Medicina Intensiva | Serum gelsolin levels in aneurismal subarachnoid hemorrhage: Preliminary results. pubmed:28365030 |
Journal of Alzheimers Disease | Urine-Based Biomarkers for Alzheimer's Disease Identified Through Coupling Computational and Experimental Methods Pubmed:30040720 |
World Journal of Pediatrics | Plasma gelsolin level predicts acute kidney injury after cardiopulmonary bypass in infants and young children Pubmed:29427164 |
Biomedicines | Production and Secretion of Gelsolin by Both Human Macrophage-and Fibroblast-like Synoviocytes and GSN Modulation in the Synovial Fluid of Patients with Various … Pubmed:35327525 |
Catalog No. | Related products for research use of Homo sapiens (Human) Organism species | Applications (RESEARCH USE ONLY!) |
RPA372Hu02 | Recombinant Gelsolin (GSN) | Positive Control; Immunogen; SDS-PAGE; WB. |
RPA372Hu05 | Recombinant Gelsolin (GSN) | Positive Control; Immunogen; SDS-PAGE; WB. |
APA372Hu61 | Active Gelsolin (GSN) | Cell culture; Activity Assays. |
RPA372Hu06 | Recombinant Gelsolin (GSN) | Positive Control; Immunogen; SDS-PAGE; WB. |
RPA372Hu03 | Recombinant Gelsolin (GSN) | Positive Control; Immunogen; SDS-PAGE; WB. |
RPA372Hu04 | Recombinant Gelsolin (GSN) | Positive Control; Immunogen; SDS-PAGE; WB. |
RPA372Hu01 | Recombinant Gelsolin (GSN) | Positive Control; Immunogen; SDS-PAGE; WB. |
EPA372Hu61 | Eukaryotic Gelsolin (GSN) | Positive Control; Immunogen; SDS-PAGE; WB. |
CPA372Hu21 | OVA Conjugated Gelsolin (GSN) | Immunogen; SDS-PAGE; WB. |
PAA372Hu05 | Polyclonal Antibody to Gelsolin (GSN) | WB; IHC; ICC; IP. |
PAA372Hu04 | Polyclonal Antibody to Gelsolin (GSN) | WB; IHC; ICC; IP. |
PAA372Hu06 | Polyclonal Antibody to Gelsolin (GSN) | WB; IHC; ICC; IP. |
PAA372Hu03 | Polyclonal Antibody to Gelsolin (GSN) | WB; IHC; ICC; IP. |
PAA372Hu02 | Polyclonal Antibody to Gelsolin (GSN) | WB; IHC; ICC; IP. |
PAA372Hu01 | Polyclonal Antibody to Gelsolin (GSN) | WB; IHC; ICC; IP. |
PAA372Hu08 | Polyclonal Antibody to Gelsolin (GSN) | WB; IHC; ICC; IP. |
LAA372Hu71 | Biotin-Linked Polyclonal Antibody to Gelsolin (GSN) | WB; IHC; ICC. |
MAA372Hu27 | Monoclonal Antibody to Gelsolin (GSN) | WB; IHC; ICC; IP. |
MAA372Hu29 | Monoclonal Antibody to Gelsolin (GSN) | WB; IHC; ICC; IP. |
MAA372Hu22 | Monoclonal Antibody to Gelsolin (GSN) | WB; IHC; ICC; IP. |
MAA372Hu24 | Monoclonal Antibody to Gelsolin (GSN) | WB; IHC |
MAA372Hu21 | Monoclonal Antibody to Gelsolin (GSN) | WB; IHC; ICC; IP. |
MAA372Hu23 | Monoclonal Antibody to Gelsolin (GSN) | WB; IHC; ICC; IP. |
MAA372Hu25 | Monoclonal Antibody to Gelsolin (GSN) | WB; IHC; ICC; IP. |
MAA372Hu26 | Monoclonal Antibody to Gelsolin (GSN) | WB; IHC; ICC; IP. |
SEA372Hu | ELISA Kit for Gelsolin (GSN) | Enzyme-linked immunosorbent assay for Antigen Detection. |
WEA372Hu | Wide-range ELISA Kit for Gelsolin (GSN) | Enzyme-linked immunosorbent assay for Antigen Detection. |
SCA372Hu | CLIA Kit for Gelsolin (GSN) | Chemiluminescent immunoassay for Antigen Detection. |
LMA372Hu | Multiplex Assay Kit for Gelsolin (GSN) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |
PSA372Hu01 | Antibody Pair for Gelsolin (GSN) | ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays. |
KSA372Hu01 | ELISA Kit DIY Materials for Gelsolin (GSN) | Main materials for "Do It (ELISA Kit) Yourself". |