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Active Colony Stimulating Factor 1, Macrophage (MCSF)
CSF1; M-CSF; Lanimostim; Processed macrophage colony-stimulating factor 1
- Product No.APA090Hu63
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Buffer FormulationPBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
- Traits Freeze-dried powder
- Purity> 95%
- Isoelectric Point4.6
- ApplicationsCell culture; Activity Assays.
- Downloadn/a
- UOM 10µg50µg 200µg 1mg 5mg
- FOB
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For more details, please contact local distributors!
ACTIVITY TEST
M-CSF (Macrophage Colony-Stimulating Factor), encoded by the CSF1 gene in humans, is a crucial cytokine that regulates the survival, proliferation, and differentiation of mononuclear phagocytes, including macrophages and their precursors. It binds to its receptor, CSF1R, activating signaling pathways essential for innate immunity, tissue homeostasis, and bone remodeling. M-CSF also plays roles in inflammatory diseases and cancer, where it can promote tumor-associated macrophage infiltration, supporting tumor progression. Its functions extend beyond hematopoiesis to include roles in metabolic regulation and neural development, making it a therapeutic target for conditions like autoimmune disorders and certain cancers.Besides,IL34 has been identified as an interactor of MCSF, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human MCSF and recombinant human IL34 . Briefly, biotin-linked MCSF were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100µl were then transferred to IL34-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST 3 times and incubation with Streptavidin-HRP for 30min, then wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µl stop solution to the wells and read at 450nm immediately. The binding activity of MCSF and IL34 was shown in Figure 1, the EC50 for this effect is 0.09587µg/mL.
USAGE
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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| Magazine | Citations |
| Brain Pathology | Microglia-Derived Macrophage Colony Stimulating Factor Promotes Generation of Proinflammatory Cytokines by Astrocytes in the Periventricular White Matter in the Hypoxic Neonatal Brain Wiley: source |
| Oncotarget | miR-1207-5p suppresses lung cancer growth and metastasis by targeting CSF1 pubmed:27107415 |
| bulletin of experimental biology and medicine | Quantitative and Qualitative Characterization of Phagocytic Activity of Macrophages of Bone Marrow and Fetal Origin Pubmed: 31183654 |
| BioMed Research International | Ultrashort Wave Combined with Human Umbilical Cord Mesenchymal Stem Cell (HUC-MSC) Transplantation Inhibits NLRP3 Inflammasome and Improves?¡ 33376718 |