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Active Colony Stimulating Factor 1, Macrophage (MCSF)
CSF1; M-CSF; Lanimostim; Processed macrophage colony-stimulating factor 1
- Product No.APA090Hu62
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Buffer FormulationPBS, pH7.4, containing 5% Trehalose.
- Traits Freeze-dried powder
- Purity> 90%
- Isoelectric Point4.6
- ApplicationsCell culture; Activity Assays.
- DownloadInstruction Manual
- UOM 10µg50µg 200µg 1mg 5mg
- FOB
US$ 254
US$ 635
US$ 1270
US$ 3810
US$ 9525
For more details, please contact local distributors!
ACTIVITY TEST
Colony Stimulating Factor 1 (MCSF), also known as Macrophage Colony-Stimulating Factor (M-CSF), is a crucial hematopoietic growth factor that regulates the survival, proliferation, and differentiation of monocytes and macrophages. It binds to its receptor, MCSFR, playing a fundamental role in innate immunity, bone biology, and homeostasis. Furthermore, MCSF and Interleukin-34 (IL34) are functionally related as they are both high-affinity ligands for the MCSF receptor (MCSFR), activating similar downstream signaling pathways despite their distinct protein structures.Besides,IL34 has been identified as an interactor of MCSF, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human MCSF and recombinant human IL34. Briefly, MCSF was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to IL34-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-MCSF pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µL stop solution to the wells and read at 450/630nm immediately. The binding activity of recombinant human MCSF and recombinant human IL34 was shown in Figure 1, the EC50 for this effect is 0.0472µg/mL.
USAGE
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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| Magazine | Citations |
| Brain Pathology | Microglia-Derived Macrophage Colony Stimulating Factor Promotes Generation of Proinflammatory Cytokines by Astrocytes in the Periventricular White Matter in the Hypoxic Neonatal Brain Wiley: source |
| Oncotarget | miR-1207-5p suppresses lung cancer growth and metastasis by targeting CSF1 pubmed:27107415 |
| bulletin of experimental biology and medicine | Quantitative and Qualitative Characterization of Phagocytic Activity of Macrophages of Bone Marrow and Fetal Origin Pubmed: 31183654 |
| BioMed Research International | Ultrashort Wave Combined with Human Umbilical Cord Mesenchymal Stem Cell (HUC-MSC) Transplantation Inhibits NLRP3 Inflammasome and Improves?¡ 33376718 |