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Recombinant Glutaminase (GLS)

GLS1; Glutaminase kidney isoform, mitochondrial; K-glutaminase; L-glutamine amidohydrolase

  • Product No.RPJ026Hu01
  • Organism SpeciesHomo sapiens (Human) Same name, Different species.
  • SourceProkaryotic expression
  • HostE.coli
  • Endotoxin Level<1.0EU per 1µg (determined by the LAL method)
  • Subcellular LocationMitochondrion, Cytoplasm
  • Predicted Molecular Mass37.5kDa
  • Accurate Molecular Mass38kDa(Analysis of differences refer to the manual)
  • Residues & TagsPhe227~Leu533 with N-terminal His Tag
  • Buffer Formulation20mM Tris, 150mM NaCl, pH8.0, containing 0.01% SKL, 5% Trehalose.
  • Traits Freeze-dried powder
  • Purity> 90%
  • Isoelectric Point6.4
  • Applications Positive Control; Immunogen; SDS-PAGE; WB.
    If bio-activity of the protein is needed, please check active protein.
  • DownloadInstruction Manual
  • UOM 10µg50µg 200µg 1mg 5mg
  • FOB US$ 152  US$ 380  US$ 760  US$ 2280  US$ 5700 
    For more details, please contact local distributors!

SEQUENCE

USAGE

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
Journal of Neuroscience Sex differences in the glutamate signaling pathway in juvenile rats Pubmed:28861894
Journal of Affective Disorders Glutamatergic hypofunction in medication-free major depression: Secondary effects of affective diagnosis and relationship to peripheral glutaminase Pubmed:29544167
校刊 King's Research Portal Doi: 10.1016/j.jad.2018.02.059
bmc research notes The role of CA-125, GLS and FASN in predicting cytoreduction for epithelial ovarian cancers Pubmed: 32698888
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPJ026Hu01 Recombinant Glutaminase (GLS) Positive Control; Immunogen; SDS-PAGE; WB.
PAJ026Hu01 Polyclonal Antibody to Glutaminase (GLS) WB; IHC
MAJ026Hu21 Monoclonal Antibody to Glutaminase (GLS) WB; IHC; ICC; IP.
SEJ026Hu ELISA Kit for Glutaminase (GLS) Enzyme-linked immunosorbent assay for Antigen Detection.
LMJ026Hu Multiplex Assay Kit for Glutaminase (GLS) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
CMJ026Hu Multiplex Assay Kit for Glutaminase (GLS) ,etc. by CBA (Cytometric Bead Array) FLIA Kit for Antigen Detection.