Glutaminase (GLS)

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GLS1; Glutaminase kidney isoform, mitochondrial; K-glutaminase; L-glutamine amidohydrolase

Glutaminase (GLS)
Altered glucose metabolism in cancer cells is termed the Warburg effect, which describes the propensity of most cancer cells to take up glucose avidly and convert it primarily to lactate, despite available oxygen. Cancer cells also depend on continued mitochondrial function for metabolism, specifically glutaminolysis that catabolizes glutamine to generate ATP and lactate. Glutamine, which is highly transported into proliferating cells, is a major source of energy and nitrogen for biosynthesis, and a carbon substrate for anabolic processes in cancer cells.
This effect leads to upregulation of glutamine catabolism. Glutaminase converts glutamine to glutamate, which is further catabolized through the tricarboxylic acid cycle for the production of ATP or serves as substrate for glutathione synthesis.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPJ026Hu01 Recombinant Glutaminase (GLS) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAJ026Hu01 Polyclonal Antibody to Glutaminase (GLS) WB; IHC; ICC; IP.
MAJ026Hu21 Monoclonal Antibody to Glutaminase (GLS) WB; IHC; ICC; IP.
Assay Kits SEJ026Hu ELISA Kit for Glutaminase (GLS) Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPJ026Mu01 Recombinant Glutaminase (GLS) Positive Control; Immunogen; SDS-PAGE; WB.
RPJ026Mu02 Recombinant Glutaminase (GLS) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAJ026Mu01 Polyclonal Antibody to Glutaminase (GLS) WB; IHC
PAJ026Mu02 Polyclonal Antibody to Glutaminase (GLS) WB; IHC; ICC; IP.
Assay Kits SEJ026Mu ELISA Kit for Glutaminase (GLS) Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPJ026Ra01 Recombinant Glutaminase (GLS) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies MAJ026Ra21 Monoclonal Antibody to Glutaminase (GLS) WB; IHC; ICC; IP.
PAJ026Ra01 Polyclonal Antibody to Glutaminase (GLS) WB; IHC; ICC; IP.
Assay Kits SEJ026Ra ELISA Kit for Glutaminase (GLS) Enzyme-linked immunosorbent assay for Antigen Detection.
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  2. "Prediction of the coding sequences of unidentified human genes. XII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro."DNA Res. 5:355-364(1998) [PubMed] [Europe PMC] [Abstract]
  3. "Isolation, characterization and expression of a human brain mitochondrial glutaminase cDNA."Brain Res. Mol. Brain Res. 76:56-63(2000) [PubMed] [Europe PMC] [Abstract]
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  5. "An unappreciated role for RNA surveillance."Genome Biol. 5:R8.1-R8.16(2004) [PubMed] [Europe PMC] [Abstract]
  6. "Brain-specific BNIP-2-homology protein Caytaxin relocalises glutaminase to neurite terminals and reduces glutamate levels."J. Cell Sci. 119:3337-3350(2006) [PubMed] [Europe PMC] [Abstract]
  7. "Relative expression of mRNAs coding for glutaminase isoforms in CNS tissues and CNS tumors."Neurochem. Res. 33:808-813(2008) [PubMed] [Europe PMC] [Abstract]
  8. "A strategy for precise and large scale identification of core fucosylated glycoproteins."Mol. Cell. Proteomics 8:913-923(2009) [PubMed] [Europe PMC] [Abstract]
  9. "Lysine acetylation targets protein complexes and co-regulates major cellular functions."Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract]
  10. "Initial characterization of the human central proteome."BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
  11. "Mitochondrial localization and structure-based phosphate activation mechanism of glutaminase C with implications for cancer metabolism."Proc. Natl. Acad. Sci. U.S.A. 109:1092-1097(2012) [PubMed] [Europe PMC] [Abstract]
  12. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
  13. "Full-length human glutaminase in complex with an allosteric inhibitor."Biochemistry 50:10764-10770(2011) [PubMed] [Europe PMC] [Abstract]
  14. "Human glutaminase in complex with L-glutamate."Submitted (JUL-2011) to the PDB data bank