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Recombinant Glutamate Cysteine Ligase, Catalytic (GCLC)

GCS; GLCL; GLCLC; Gamma-glutamylcysteine synthetase; Gamma-ECS

  • Product No.RPD759Ra01
  • Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
  • SourceProkaryotic expression
  • HostE.coli
  • Endotoxin Level<1.0EU per 1µg (determined by the LAL method)
  • Subcellular LocationMitochondrion, Cytoplasm
  • Predicted Molecular Mass24kDa
  • Accurate Molecular Mass25kDa(Analysis of differences refer to the manual)
  • Residues & TagsAsp435~Ser636 (Accession # P19468) with N-terminal His Tag
  • Buffer FormulationPBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
  • Traits Freeze-dried powder
  • Purity> 95%
  • Isoelectric Point5.65
  • Applications Positive Control; Immunogen; SDS-PAGE; WB.
    If bio-activity of the protein is needed, please check active protein.
  • DownloadInstruction Manual
  • UOM 10µg50µg 200µg 1mg 5mg
  • FOB US$ 188  US$ 470  US$ 940  US$ 2820  US$ 7050 
    For more details, please contact local distributors!

SEQUENCE

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
Oxidative Medicine and Cellular Longevity Overexpression of Brg1 Alleviates Hepatic Ischemia/Reperfusion-Induced Acute Lung Injury through Antioxidative Stress Effects pubmed:28798861
Journal of Functional Foods Effects of maternal serine supplementation on high-fat diet-induced oxidative stress and epigenetic changes in promoters of glutathione synthesis-related genes in … 10.1016:j.jff.2018.05.067
Catalog No. Related products for research use of Rattus norvegicus (Rat) Organism species Applications (RESEARCH USE ONLY!)
RPD759Ra01 Recombinant Glutamate Cysteine Ligase, Catalytic (GCLC) Positive Control; Immunogen; SDS-PAGE; WB.
PAD759Ra01 Polyclonal Antibody to Glutamate Cysteine Ligase, Catalytic (GCLC) WB; IHC; ICC; IP.
MAD759Ra21 Monoclonal Antibody to Glutamate Cysteine Ligase, Catalytic (GCLC) WB; IHC; ICC; IP.
SED759Ra ELISA Kit for Glutamate Cysteine Ligase, Catalytic (GCLC) Enzyme-linked immunosorbent assay for Antigen Detection.
LMD759Ra Multiplex Assay Kit for Glutamate Cysteine Ligase, Catalytic (GCLC) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.