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Glutamate Cysteine Ligase, Catalytic (GCLC)

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GCS; GLCL; GLCLC; Gamma-glutamylcysteine synthetase; Gamma-ECS

Glutamate Cysteine Ligase, Catalytic (GCLC)
CCC D759
HGNC 4311
MGI 104990
PubMed 15988009
RGD 619868
UniProt P48506
Wiki GCLC
Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. Gamma-glutamylcysteine synthetase is the first rate-limiting enzyme in glutathione (GSH) biosynthesis.
Glutamate--cysteine ligase catalytic subunit is the catalytic subunit encodes a protein of 367 amino acids with a calculated molecular weight of 72.773 kDa and maps to chromosome 6. The regulatory subunit is derived from a different gene located on chromosome 1p22-p21. Deficiency of gamma-glutamylcysteine synthetase in human is associated with enzymopathic hemolytic anemia.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPD759Hu01 Recombinant Glutamate Cysteine Ligase, Catalytic (GCLC) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAD759Hu01 Polyclonal Antibody to Glutamate Cysteine Ligase, Catalytic (GCLC) WB; IHC; ICC; IP.
Assay Kits n/a CLIA Kit for Glutamate Cysteine Ligase, Catalytic (GCLC) CLIA Kit Customized Service Offer
n/a ELISA Kit for Glutamate Cysteine Ligase, Catalytic (GCLC) ELISA Kit Customized Service Offer

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPD759Mu01 Recombinant Glutamate Cysteine Ligase, Catalytic (GCLC) Positive Control; Immunogen; SDS-PAGE; WB.
RPD759Mu02 Recombinant Glutamate Cysteine Ligase, Catalytic (GCLC) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAD759Mu01 Polyclonal Antibody to Glutamate Cysteine Ligase, Catalytic (GCLC) WB; IHC; ICC; IP.
PAD759Mu02 Polyclonal Antibody to Glutamate Cysteine Ligase, Catalytic (GCLC) WB; IHC; ICC; IP.
Assay Kits SED759Mu ELISA Kit for Glutamate Cysteine Ligase, Catalytic (GCLC) Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPD759Ra01 Recombinant Glutamate Cysteine Ligase, Catalytic (GCLC) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAD759Ra01 Polyclonal Antibody to Glutamate Cysteine Ligase, Catalytic (GCLC) WB; IHC; ICC; IP.
MAD759Ra21 Monoclonal Antibody to Glutamate Cysteine Ligase, Catalytic (GCLC) WB; IHC; ICC; IP.
Assay Kits SED759Ra ELISA Kit for Glutamate Cysteine Ligase, Catalytic (GCLC) Enzyme-linked immunosorbent assay for Antigen Detection.
  1. "Cloning and nucleotide sequence of a full-length cDNA for human liver gamma-glutamylcysteine synthetase."Biochem. Biophys. Res. Commun. 185:29-35(1992) [PubMed] [Europe PMC] [Abstract]
  2. "The DNA sequence and analysis of human chromosome 6." Nature 425:805-811(2003) [PubMed] [Europe PMC] [Abstract]
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  4. "Identification of a putative antioxidant response element in the 5'-flanking region of the human gamma-glutamylcysteine synthetase heavy subunit gene."Biochem. Biophys. Res. Commun. 209:227-233(1995) [PubMed] [Europe PMC] [Abstract]
  5. "The molecular basis of a case of gamma-glutamylcysteine synthetase deficiency."Blood 94:2890-2894(1999) [PubMed] [Europe PMC] [Abstract]
  6. "Expression and purification of human gamma-glutamylcysteine synthetase."Protein Expr. Purif. 13:268-276(1998) [PubMed] [Europe PMC] [Abstract]
  7. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
  8. "Initial characterization of the human central proteome."BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
  9. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
  10. "A missense mutation in the heavy subunit of gamma-glutamylcysteine synthetase gene causes hemolytic anemia."Blood 95:2193-2196(2000) [PubMed] [Europe PMC] [Abstract]
  11. "A novel missense mutation in the gamma-glutamylcysteine synthetase catalytic subunit gene causes both decreased enzymatic activity and glutathione production."Blood 102:725-730(2003) [PubMed] [Europe PMC] [Abstract]