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Recombinant Collagen Type II Alpha 1 (COL2a1)

COL2-A1; COL2A1; SEDC; Chondrocalcin; Collagen Alpha-1(II)Chain; Primary Osteoarthritis,Spondyloepiphyseal Dysplasia,Congenital

  • Product No.RPD194Hu02
  • Organism SpeciesHomo sapiens (Human) Same name, Different species.
  • SourceProkaryotic expression
  • HostE.coli
  • Endotoxin Level<1.0EU per 1µg (determined by the LAL method)
  • Subcellular LocationSecreted, Extracellular matrix
  • Predicted Molecular Mass19.4kDa
  • Accurate Molecular Mass29kDa(Analysis of differences refer to the manual)
  • Residues & TagsGln26~Ala181 with N-terminal His Tag
  • Buffer Formulation20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
  • Traits Freeze-dried powder
  • Purity> 95%
  • Isoelectric Point4.2
  • Applications Positive Control; Immunogen; SDS-PAGE; WB.
    If bio-activity of the protein is needed, please check active protein.
  • DownloadInstruction Manual
  • UOM 10µg50µg 200µg 1mg 5mg
  • FOB US$ 181  US$ 453  US$ 906  US$ 2718  US$ 6795 
    For more details, please contact local distributors!

SEQUENCE

USAGE

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
Materials Science & Engineering C-Materials for Biological Applications Spatially controlled functional group grafting of silk films to induce osteogenic and chondrogenic differentiation of human mesenchymal stem cells Pubmed:30033315
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPD194Hu01 Recombinant Collagen Type II Alpha 1 (COL2a1) Positive Control; Immunogen; SDS-PAGE; WB.
RPD194Hu02 Recombinant Collagen Type II Alpha 1 (COL2a1) Positive Control; Immunogen; SDS-PAGE; WB.
PAD194Hu01 Polyclonal Antibody to Collagen Type II Alpha 1 (COL2a1) WB; IHC; ICC; IP.
MAD194Hu22 Monoclonal Antibody to Collagen Type II Alpha 1 (COL2a1) WB; IHC; ICC; IP.
MAD194Hu21 Monoclonal Antibody to Collagen Type II Alpha 1 (COL2a1) WB; IHC; ICC; IP.
SED194Hu ELISA Kit for Collagen Type II Alpha 1 (COL2a1) Enzyme-linked immunosorbent assay for Antigen Detection.
LMD194Hu Multiplex Assay Kit for Collagen Type II Alpha 1 (COL2a1) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.