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Monoclonal Antibody to Collagen Type II Alpha 1 (COL2a1)

COL2-A1; COL2A1; SEDC; Chondrocalcin; Collagen Alpha-1(II)Chain; Primary Osteoarthritis,Spondyloepiphyseal Dysplasia,Congenital

  • Product No.MAD194Hu21
  • Organism SpeciesHomo sapiens (Human) Same name, Different species.
  • SourceMonoclonal antibody preparation
  • HostMouse
  • Potency (Clone Number)n/a
  • Ig Isotype IgG
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Immunogen n/a
  • Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
  • TraitsLiquid
  • Concentration1mg/mL
  • Organism Species Moren/a
  • ApplicationsWB; IHC; ICC; IP.
    If the antibody is used in flow cytometry, please check FCM antibodies.
  • Downloadn/a
  • UOM 20µl100µl 200µl 1ml 10ml
  • FOB US$ 111  US$ 259  US$ 370  US$ 925  US$ 3700 
    For more details, please contact local distributors!

SPECIFITY

The antibody is a mouse monoclonal antibody raised against COL2a1. It has been selected for its ability to recognize COL2a1 in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.2-2µg/mL;1:500-5000
Immunohistochemistry: 5-20µg/mL;1:50-200
Immunocytochemistry: 5-20µg/mL;1:50-200
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
Materials Science & Engineering C-Materials for Biological Applications Spatially controlled functional group grafting of silk films to induce osteogenic and chondrogenic differentiation of human mesenchymal stem cells Pubmed:30033315
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPD194Hu01 Recombinant Collagen Type II Alpha 1 (COL2a1) Positive Control; Immunogen; SDS-PAGE; WB.
RPD194Hu02 Recombinant Collagen Type II Alpha 1 (COL2a1) Positive Control; Immunogen; SDS-PAGE; WB.
PAD194Hu01 Polyclonal Antibody to Collagen Type II Alpha 1 (COL2a1) WB; IHC; ICC; IP.
MAD194Hu22 Monoclonal Antibody to Collagen Type II Alpha 1 (COL2a1) WB; IHC; ICC; IP.
MAD194Hu21 Monoclonal Antibody to Collagen Type II Alpha 1 (COL2a1) WB; IHC; ICC; IP.
SED194Hu ELISA Kit for Collagen Type II Alpha 1 (COL2a1) Enzyme-linked immunosorbent assay for Antigen Detection.
LMD194Hu Multiplex Assay Kit for Collagen Type II Alpha 1 (COL2a1) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.