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Recombinant O-6-Methylguanine DNA Methyltransferase (MGMT)

Methylated-DNA--Protein-Cysteine Methyltransferase; 6-O-methylguanine-DNA methyltransferase; Methylated-DNA--protein-cysteine methyltransferase

  • Product No.RPC027Hu01
  • Organism SpeciesHomo sapiens (Human) Same name, Different species.
  • SourceProkaryotic expression
  • HostE.coli
  • Endotoxin Level<1.0EU per 1µg (determined by the LAL method)
  • Subcellular LocationNucleus
  • Predicted Molecular Mass25.3kDa
  • Accurate Molecular Mass25kDa(Analysis of differences refer to the manual)
  • Residues & TagsMet1~Asn207 with N-terminal His Tag
  • Buffer Formulation20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
  • Traits Freeze-dried powder
  • Purity> 97%
  • Isoelectric Point8.3
  • Applications Positive Control; Immunogen; SDS-PAGE; WB.
    If bio-activity of the protein is needed, please check active protein.
  • DownloadInstruction Manual
  • UOM 10µg50µg 200µg 1mg 5mg
  • FOB US$ 176  US$ 440  US$ 880  US$ 2640  US$ 6600 
    For more details, please contact local distributors!

SEQUENCE

USAGE

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
Biotechnology and Bioengineering Targeted nitric oxide delivery preferentially induces glioma cell chemosensitivity via altered p53 and O(6) -Methylguanine-DNA Methyltransferase activity PubMed: 23125026
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPC027Hu01 Recombinant O-6-Methylguanine DNA Methyltransferase (MGMT) Positive Control; Immunogen; SDS-PAGE; WB.
PAC027Hu01 Polyclonal Antibody to O-6-Methylguanine DNA Methyltransferase (MGMT) WB,IHC
MAC027Hu22 Monoclonal Antibody to O-6-Methylguanine DNA Methyltransferase (MGMT) WB; IHC; ICC; IP.
SEC027Hu ELISA Kit for O-6-Methylguanine DNA Methyltransferase (MGMT) Enzyme-linked immunosorbent assay for Antigen Detection.
LMC027Hu Multiplex Assay Kit for O-6-Methylguanine DNA Methyltransferase (MGMT) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.