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O-6-Methylguanine DNA Methyltransferase (MGMT)

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Methylated-DNA--Protein-Cysteine Methyltransferase; 6-O-methylguanine-DNA methyltransferase; Methylated-DNA--protein-cysteine methyltransferase

O-6-Methylguanine DNA Methyltransferase (MGMT)
CCC C027
HGNC 7059
MGI 96977
PubMed 21745538
RGD 3087
UniProt P16455
Wiki MGMT

O(6)-alkylguanine is the major mutagenic and carcinogenic lesion in DNA induced by simple alkylating mutagens because of its preference for pairing with thymine during DNA replication. This adduct in DNA is removed by a ubiquitous and unique repair protein, O(6)-methylguanine-DNA methyltransferase (EC 2.1.1.63).

Methylated-DNA--protein-cysteine methyltransferase, unlike true enzymes, accepts the alkyl group from the lesion in a stoichiometric second-order reaction. The methyl-acceptor residue is cysteine. Tano et al. (1990) cloned the cDNA of the human MGMT gene in an expression vector on the basis of its rescue of a methyltransferase-deficient E. coli host.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPC027Hu01 Recombinant O-6-Methylguanine DNA Methyltransferase (MGMT) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAC027Hu01 Polyclonal Antibody to O-6-Methylguanine DNA Methyltransferase (MGMT) WB,IHC
MAC027Hu22 Monoclonal Antibody to O-6-Methylguanine DNA Methyltransferase (MGMT) WB; IHC; ICC; IP.
Assay Kits SEC027Hu ELISA Kit for O-6-Methylguanine DNA Methyltransferase (MGMT) Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant O-6-Methylguanine DNA Methyltransferase (MGMT) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to O-6-Methylguanine DNA Methyltransferase (MGMT) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to O-6-Methylguanine DNA Methyltransferase (MGMT) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for O-6-Methylguanine DNA Methyltransferase (MGMT) CLIA Kit Customized Service Offer
n/a ELISA Kit for O-6-Methylguanine DNA Methyltransferase (MGMT) ELISA Kit Customized Service Offer

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPC027Ra01 Recombinant O-6-Methylguanine DNA Methyltransferase (MGMT) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAC027Ra01 Polyclonal Antibody to O-6-Methylguanine DNA Methyltransferase (MGMT) WB; IHC; ICC; IP.
MAC027Ra21 Monoclonal Antibody to O-6-Methylguanine DNA Methyltransferase (MGMT) WB; IHC; ICC; IP.
Assay Kits SEC027Ra ELISA Kit for O-6-Methylguanine DNA Methyltransferase (MGMT) Enzyme-linked immunosorbent assay for Antigen Detection.
  1. "Isolation and structural characterization of a cDNA clone encoding the human DNA repair protein for O6-alkylguanine."Proc. Natl. Acad. Sci. U.S.A. 87:686-690(1990) [PubMed] [Europe PMC] [Abstract]
  2. "cDNA cloning and chromosomal assignment of the human O6-methylguanine-DNA methyltransferase. cDNA expression in Escherichia coli and gene expression in human cells."J. Biol. Chem. 265:9563-9569(1990) [PubMed] [Europe PMC] [Abstract]
  3. "Purification, structure, and biochemical properties of human O6-methylguanine-DNA methyltransferase."J. Biol. Chem. 265:14754-14762(1990) [PubMed] [Europe PMC] [Abstract]
  4. "Expression and cloning of complementary DNA for a human enzyme that repairs O6-methylguanine in DNA."J. Mol. Biol. 213:739-747(1990) [PubMed] [Europe PMC] [Abstract]
  5. "The DNA sequence and comparative analysis of human chromosome 10." Nature 429:375-381(2004) [PubMed] [Europe PMC] [Abstract]
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  7. "Structural and immunological comparison of indigenous human O6-methylguanine-DNA methyltransferase with that encoded by a cloned cDNA."J. Biol. Chem. 266:1064-1070(1991) [PubMed] [Europe PMC] [Abstract]
  8. "Specificities of human, rat and E. coli O6-methylguanine-DNA methyltransferases towards the repair of O6-methyl and O6-ethylguanine in DNA."Nucleic Acids Res. 22:1613-1619(1994) [PubMed] [Europe PMC] [Abstract]
  9. "Mutations in human O6-alkylguanine-DNA alkyltransferase imparting resistance to O6-benzylguanine."Cancer Res. 54:6221-6227(1994) [PubMed] [Europe PMC] [Abstract]
  10. "Alteration of arginine-128 to alanine abolishes the ability of human O6-alkylguanine-DNA alkyltransferase to repair methylated DNA but has no effect on its reaction with O6-benzylguanine."Biochemistry 34:7113-7119(1995) [PubMed] [Europe PMC] [Abstract]
  11. "The role of tyrosine-158 in O6-alkylguanine-DNA alkyltransferase activity."Carcinogenesis 16:1637-1642(1995) [PubMed] [Europe PMC] [Abstract]
  12. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
  13. "Active and alkylated human AGT structures: a novel zinc site, inhibitor and extrahelical base binding."EMBO J. 19:1719-1730(2000) [PubMed] [Europe PMC] [Abstract]
  14. "Crystal structure of the human O(6)-alkylguanine-DNA alkyltransferase."Nucleic Acids Res. 28:393-401(2000) [PubMed] [Europe PMC] [Abstract]
  15. "The structure of the human AGT protein bound to DNA and its implications for damage detection."J. Mol. Biol. 350:657-666(2005) [PubMed] [Europe PMC] [Abstract]