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Recombinant Enolase 1 (ENO1)

NNE; ENO1L1; MPB1; PPH; Alpha Enolase; Enolase 1; Phosphopyruvate hydratase; Plasminogen-binding protein; 2-phospho-D-glycerate hydro-lyase; C-myc promoter-binding

  • Product No.RPB449Hu01
  • Organism SpeciesHomo sapiens (Human) Same name, Different species.
  • SourceProkaryotic expression
  • HostE.coli
  • Endotoxin Level<1.0EU per 1µg (determined by the LAL method)
  • Subcellular LocationMembrane, Nucleus, Cytoplasm
  • Predicted Molecular Mass11.3kDa
  • Accurate Molecular Mass13kDa(Analysis of differences refer to the manual)
  • Residues & TagsPro267~Leu354 with N-terminal His Tag
  • Buffer Formulation20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
  • Traits Freeze-dried powder
  • Purity> 95%
  • Isoelectric Point5.8
  • Applications Positive Control; Immunogen; SDS-PAGE; WB.
    If bio-activity of the protein is needed, please check active protein.
  • DownloadInstruction Manual
  • UOM 10µg50µg 200µg 1mg 5mg
  • FOB US$ 168  US$ 420  US$ 840  US$ 2520  US$ 6300 
    For more details, please contact local distributors!

SEQUENCE

USAGE

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
Analytical Chemistry Diagnostic detection of human lung cancer-associated antigen using a gold nanoparticle-based electrochemical immunosensor PubMed: 20557064
Translational Research Proteomic analysis of fine-needle aspiration in differential diagnosis of thyroid nodules Pubmed:27172385
Journal of Proteomics Putative salivary biomarkers useful to differentiate patients with fibromyalgia Pubmed:29654921
BioMed Research International The Diagnostic Accuracy of Combined Enolase/Cr, CA125, and CA19-9 in the Detection of Endometriosis Pubmed: 33062681
Cancer Cell International Bufalin Induced Mitochondrial Dysfunction Promotes Apoptosis of Glioma Cells by Regulating Annexin A2 and DRP1 Proteins
Cancer Cell Int Bufalin induces mitochondrial dysfunction and promotes apoptosis of glioma cells by regulating Annexin A2 and DRP1 protein expression 34376212
Clin Proteomics Serum proteomics unveil characteristic protein diagnostic biomarkers and signaling pathways in patients with esophageal squamous cell carcinoma Pubmed:35610567
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPB449Hu03 Recombinant Enolase 1 (ENO1) Positive Control; Immunogen; SDS-PAGE; WB.
RPB449Hu02 Recombinant Enolase 1 (ENO1) Positive Control; Immunogen; SDS-PAGE; WB.
RPB449Hu01 Recombinant Enolase 1 (ENO1) Positive Control; Immunogen; SDS-PAGE; WB.
PAB449Hu02 Polyclonal Antibody to Enolase 1 (ENO1) WB; IHC; ICC; IP.
PAB449Hu03 Polyclonal Antibody to Enolase 1 (ENO1) WB; IHC; ICC; IP.
PAB449Hu01 Polyclonal Antibody to Enolase 1 (ENO1) WB; IHC; ICC; IP.
LAB449Hu71 Biotin-Linked Polyclonal Antibody to Enolase 1 (ENO1) WB; IHC; ICC.
MAB449Hu21 Monoclonal Antibody to Enolase 1 (ENO1) WB; IHC; ICC; IP.
MAB449Hu22 Monoclonal Antibody to Enolase 1 (ENO1) WB; IHC.
SEB449Hu ELISA Kit for Enolase 1 (ENO1) Enzyme-linked immunosorbent assay for Antigen Detection.
AEB449Hu ELISA Kit for Anti-Enolase 1 Antibody (Anti-ENO1) Enzyme-linked immunosorbent assay for Antibody Detection.
SCB449Hu CLIA Kit for Enolase 1 (ENO1) Chemiluminescent immunoassay for Antigen Detection.