Packages (Simulation)
Image (I)
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DAB staining on IHC-P; Sample: Human Kidney Tissue; Primary Ab: 20µg/ml Mouse Anti-Human NNE Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
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DAB staining on IHC-P;
Sample: Human Kidney Tissue
Primary Ab: 20µg/ml Mouse Anti-Human ENO1 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
Image (II)
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Western Blot; Sample: Lane1: Rat Small intestine lysate; Lane2: Bovine Cerebrum lysate; Lane3: Hela cell lysate; Lane4: PC3 cell lysate Primary Ab: 2µg/ml Mouse Anti-Human NNE Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
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Instruction Manual
Certificate
Monoclonal Antibody to Enolase 1 (ENO1)
NNE; ENO1L1; MPB1; PPH; Alpha Enolase; Enolase 1; Phosphopyruvate hydratase; Plasminogen-binding protein; 2-phospho-D-glycerate hydro-lyase; C-myc promoter-binding
- Product No.MAB449Hu22
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- SourceMonoclonal antibody preparation
- HostMouse
- Potency (Clone Number)C2
- Ig Isotype IgG1 Kappa
- PurificationProtein A + Protein G affinity chromatography
- LabelNone
- Immunogen RPB449Hu02-Recombinant Enolase 1 (ENO1)
- Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
- TraitsLiquid
- Concentration1mg/ml
- Organism Species MoreRattus norvegicus (Rat), Sus scrofa; Porcine (Pig), Bos taurus; Bovine (Cattle)
- ApplicationsWB; IHC.
If the antibody is used in flow cytometry, please check FCM antibodies. - DownloadInstruction Manual
- UOM 20µl100µl 200µl 1ml 10ml
- FOB
US$ 106
US$ 246
US$ 352
US$ 880
US$ 3520
For more details, please contact local distributors!
SPECIFITY
The antibody is a mouse monoclonal antibody raised against ENO1. It has been selected for its ability to recognize ENO1 in immunohistochemical staining and western blotting.
USAGE
Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
GIVEAWAYS
INCREMENT SERVICES
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Antibody Labeling Customized Service
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Protein A/G Purification Column
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Staining Solution for Cells and Tissue
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Positive Control for Antibody
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Tissue/Sections Customized Service
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Phosphorylated Antibody Customized Service
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Western Blot (WB) Experiment Service
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Immunohistochemistry (IHC) Experiment Service
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Immunocytochemistry (ICC) Experiment Service
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Flow Cytometry (FCM) Experiment Service
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Immunoprecipitation (IP) Experiment Service
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Immunofluorescence (IF) Experiment Service
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Buffer
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DAB Chromogen Kit
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SABC Kit
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Long-arm Biotin Labeling Kit
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Real Time PCR Experimental Service
| Magazine | Citations |
| Analytical Chemistry | Diagnostic detection of human lung cancer-associated antigen using a gold nanoparticle-based electrochemical immunosensor PubMed: 20557064 |
| Translational Research | Proteomic analysis of fine-needle aspiration in differential diagnosis of thyroid nodules Pubmed:27172385 |
| Journal of Proteomics | Putative salivary biomarkers useful to differentiate patients with fibromyalgia Pubmed:29654921 |
| BioMed Research International | The Diagnostic Accuracy of Combined Enolase/Cr, CA125, and CA19-9 in the Detection of Endometriosis Pubmed: 33062681 |
| Cancer Cell International | Bufalin Induced Mitochondrial Dysfunction Promotes Apoptosis of Glioma Cells by Regulating Annexin A2 and DRP1 Proteins |
| Cancer Cell Int | Bufalin induces mitochondrial dysfunction and promotes apoptosis of glioma cells by regulating Annexin A2 and DRP1 protein expression 34376212 |
| Clin Proteomics | Serum proteomics unveil characteristic protein diagnostic biomarkers and signaling pathways in patients with esophageal squamous cell carcinoma Pubmed:35610567 |
| Catalog No. | Related products for research use of Homo sapiens (Human) Organism species | Applications (RESEARCH USE ONLY!) |
| RPB449Hu03 | Recombinant Enolase 1 (ENO1) | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPB449Hu02 | Recombinant Enolase 1 (ENO1) | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPB449Hu01 | Recombinant Enolase 1 (ENO1) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAB449Hu02 | Polyclonal Antibody to Enolase 1 (ENO1) | WB; IHC; ICC; IP. |
| PAB449Hu03 | Polyclonal Antibody to Enolase 1 (ENO1) | WB; IHC; ICC; IP. |
| PAB449Hu01 | Polyclonal Antibody to Enolase 1 (ENO1) | WB; IHC; ICC; IP. |
| LAB449Hu71 | Biotin-Linked Polyclonal Antibody to Enolase 1 (ENO1) | WB; IHC; ICC. |
| MAB449Hu21 | Monoclonal Antibody to Enolase 1 (ENO1) | WB; IHC; ICC; IP. |
| MAB449Hu22 | Monoclonal Antibody to Enolase 1 (ENO1) | WB; IHC. |
| SEB449Hu | ELISA Kit for Enolase 1 (ENO1) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| AEB449Hu | ELISA Kit for Anti-Enolase 1 Antibody (Anti-ENO1) | Enzyme-linked immunosorbent assay for Antibody Detection. |
| SCB449Hu | CLIA Kit for Enolase 1 (ENO1) | Chemiluminescent immunoassay for Antigen Detection. |