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Recombinant Glypican 4 (GPC4)

K-Glypican; Glypican Proteoglycan 4; Secreted glypican-4

  • Product No.RPA998Ra01
  • Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
  • SourceProkaryotic expression
  • HostE.coli
  • Endotoxin Level<1.0EU per 1µg (determined by the LAL method)
  • Subcellular LocationMembrane
  • Predicted Molecular Mass28.8kDa
  • Accurate Molecular Mass27kDa(Analysis of differences refer to the manual)
  • Residues & TagsAla222~Leu468 with N-terminal His Tag
  • Buffer Formulation20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
  • Traits Freeze-dried powder
  • Purity> 95%
  • Isoelectric Point7.1
  • Applications Positive Control; Immunogen; SDS-PAGE; WB.
    If bio-activity of the protein is needed, please check active protein.
  • DownloadInstruction Manual
  • UOM 10µg50µg 200µg 1mg 5mg
  • FOB US$ 200  US$ 500  US$ 1000  US$ 3000  US$ 7500 
    For more details, please contact local distributors!

SEQUENCE

USAGE

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
PLoS One. Identification of adipokine clusters related to parameters of fat mass, insulin sensitivity and inflammation Pubmed:Pmc4072672
Aspects of fluid therapy in the critically ill. Experimental and clinical studies on fluid therapy in the inflammatory conditions. 250690_2_G5_Svajunas_S.pdf
Intensive Care Medicine Experimental Elevated plasma glypicans are associated with organ failure in patients with infection Pubmed: 30618011
Crit Care Albumin infusion rate and plasma volume expansion: a randomized clinical trial in postoperative patients after major surgery Pubmed: 31138247
J Endocrinol Invest Serum glypican4 and glycosylphosphatidylinositol-specific phospholipase D levels are associated with adipose tissue insulin resistance in obese subjects with … Pubmed: 32816247
Heparin Binding Protein and Endothelial Glycocalyx Markers in Severe COVID-19–A Prospective Observational Cohort Study
Sci Rep Glypican-4 in pregnancy and its relation to glucose metabolism, insulin resistance and gestational diabetes mellitus status 34903856
Catalog No. Related products for research use of Rattus norvegicus (Rat) Organism species Applications (RESEARCH USE ONLY!)
APA998Ra01 Active Glypican 4 (GPC4) Cell culture; Activity Assays.
RPA998Ra01 Recombinant Glypican 4 (GPC4) Positive Control; Immunogen; SDS-PAGE; WB.
PAA998Ra01 Polyclonal Antibody to Glypican 4 (GPC4) WB; IHC; ICC; IP.
SEA998Ra ELISA Kit for Glypican 4 (GPC4) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA998Ra Multiplex Assay Kit for Glypican 4 (GPC4) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.