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Recombinant Peroxisome Proliferator Activated Receptor Gamma (PPARg)

PPAR-G; PPARG1; PPARG2; NR1C3; Glitazone Receptor; Nuclear Receptor Subfamily 1 Group C Member 3

  • Product No.RPA886Ra01
  • Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
  • SourceProkaryotic expression
  • HostE.coli
  • Endotoxin Level<1.0EU per 1µg (determined by the LAL method)
  • Subcellular LocationNucleus, Cytoplasm
  • Predicted Molecular Mass22.3kDa
  • Accurate Molecular Mass22kDa(Analysis of differences refer to the manual)
  • Residues & TagsGln311~Leu493 with N-terminal His Tag
  • Buffer Formulation20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
  • Traits Freeze-dried powder
  • Purity> 95%
  • Isoelectric Point5.5
  • Applications Positive Control; Immunogen; SDS-PAGE; WB.
    If bio-activity of the protein is needed, please check active protein.
  • DownloadInstruction Manual
  • UOM 10µg50µg 200µg 1mg 5mg
  • FOB US$ 192  US$ 480  US$ 960  US$ 2880  US$ 7200 
    For more details, please contact local distributors!

SEQUENCE

USAGE

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
Neuroscience Letters Elevated levels of PPAR-gamma in the cerebrospinal fluid of patients with multiple sclerosis Pubmed: 24021801
J Cell Biochem. Nonivamide enhances miRNA let‐7d expression and decreases adipogenesis PPARγ expression in 3T3‐L1 cells Pubmed:25704235
Osteoarthritis Cartilage Establishment of a rabbit model to study the influence of advanced glycation end products accumulation on osteoarthritis and the protective effect of pioglitazone PubMed: 26321377
J Clin Diagn Res. Evaluation of Protein Kinase Cβ and PPARγ Activity in Diabetic Rats Supplemented with Momordica charantia pmc:PMC4866090
Journal of Clinical&Diagnostic Research Evaluation of Protein Kinase Cβ and PPARγ Activity in Diabetic RatsSupplemented with Momordica charantia. pubmed:27190792
Osteoarthritis and Cartilage Establishment of a rabbit model to study the influence of advanced glycation end productsaccumulation on osteoarthritis and the protective effect of pioglitazone. pubmed:26321377
Neuroscience Letters Unlike PPARgamma, neither other PPARs nor PGC-1alpha is elevated in the cerebrospinal fluid of patients with multiple sclerosis pubmed:28483651
European Journal of Immunology Engulfment of Hb‐activated platelets differentiates monocytes into pro‐inflammatory macrophages in PNH patients Pubmed:29677388
Histochemistry and Cell Biology Simpson–Golabi–Behmel syndrome human adipocytes reveal a changing phenotype throughout differentiation Pubmed:29574488
molecular and cellular biochemistry Maternal omega-3 fatty acids and vitamin E improve placental angiogenesis in late-onset but not early-onset preeclampsia Pubmed: 31420792
Nutrients.  Hyperglycemia Changes Expression of Key Adipogenesis Markers (C/EBPα and PPARᵞ) and Morphology of Differentiating Human Visceral Adipocytes Pubmed: 31398873
journal of biochemical and molecular toxicology Indomethacin and juglone inhibit inflammatory molecules to induce apoptosis in colon cancer cells Pubmed: 31916655
J Cosmet Dermatol In©\vitro effect of pine bark extract on melanin synthesis, tyrosinase activity, production of endothelin©\1 and PPAR in cultured melanocytes exposed to Ultraviolet?¡­ 33960120
American Chemical Society TRPA1 Agonist Cinnamaldehyde Decreases Adipogenesis in 3T3-L1 Cells More Potently than the Non-agonist Structural Analog Cinnamyl Isobutyrate 33403292
PLoS One Quantitative real-time measurement of endothelin-1-induced contraction in single non-activated hepatic stellate cells 34343209
Prostaglandins Leukot Essent Fatty Acids Maternal Vitamin D Deficiency Reduces Docosahexaenoic Acid, Placental Growth Factor and Peroxisome Proliferator Activated Receptor Gamma levels in the Pup … 34768025
Food and Chemical Toxicology Melatonin attenuates cisplatin-induced acute kidney injury in mice: Involvement of PPARα and fatty acid oxidation Pubmed:35367536
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