Recombinant Hypoxia Inducible Factor 1 Alpha (HIF1a)
HIF1-A; MOP1; PASD8; Basic Helix-Loop-Helix Transcription Factor; ARNT-interacting protein; Basic-helix-loop-helix-PAS protein MOP1; Class E basic helix-loop-helix protein 78
- Product No.RPA798Ra01
- Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- SourceProkaryotic expression
- HostE.coli
- Endotoxin Level<1.0EU per 1µg (determined by the LAL method)
- Subcellular LocationNucleus, Cytoplasm
- Predicted Molecular Mass15.4kDa
- Accurate Molecular Mass16kDa(Analysis of differences refer to the manual)
- Residues & TagsPro216~Val336 with N-terminal His Tag
- Buffer FormulationPBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
- Traits Freeze-dried powder
- Purity> 90%
- Isoelectric Point5.6
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Applications
Positive Control; Immunogen; SDS-PAGE; WB.
If bio-activity of the protein is needed, please check active protein. - DownloadInstruction Manual
- UOM 10µg50µg 200µg 1mg 5mg
- FOB
US$ 152
For more details, please contact local distributors! US$ 380 US$ 760 US$ 2280 US$ 5700
SEQUENCE
![](/upload/product/pictures/RPA798Ra01-201702081650545480.jpg)
USAGE
Reconstitute in PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
GIVEAWAYS
INCREMENT SERVICES
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Endotoxin Removal Kit
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BCA Protein Quantification Kit
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Protein Labeling Customized Service
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Molecular Mass Marker for Protein
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Recombinant Protein Customized Service
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Monoclonal Antibody Customized Service
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Polyclonal Antibody Customized Service
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Protein Activity Test Experiment Service
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Immunoprecipitation (IP) Experiment Service
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Buffer
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Endotoxin Removal Kit II
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Real Time PCR Experimental Service
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Spike RBD Protein (S-RBD)
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Protein G
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Protein A
Magazine | Citations |
Cancer Chemotherapy and Pharmacology | Quercetin greatly improved therapeutic index of doxorubicin against 4T1 breast cancer by its opposing effects on HIF-1α in tumor and normal cells. PubMed: 19466611 |
European Journal of Pharmacology | Baicalin suppresses lung carcinoma and lung metastasis by SOD mimic and HIF-1α inhibition ScienceDirect: S0014299909011352 |
PLoS ONE | Lactate Dehydrogenase 5 Expression in Non-Hodgkin Lymphoma Is Associated with the Induced Hypoxia Regulated Protein and Poor Prognosis PubMed: PMC3781153 |
International Journal of Molecular Medicine | Selective inhibition of PKCβ2 preserves cardiac function after myocardial infarction and is associated with improved angiogenesis of ischemic myocardium in diabetic rats Pubmed: 24002353 |
Canadian Journal of Physiology and Pharmacology | Modulation of arginine and asymmetric dimethylarginine concentrations in liver and plasma by exogenous hydrogen sulfide in LPS-induced endotoxemia Pubmed: 24289078 |
US8569372 B2 | Method for preventing or treating a disease, disorder or condition induced by retina ischemia US8569372 |
J Surg Res. | Effects of adrenomedullin and vascular endothelial growth factor on ischemia/reperfusion injury in skeletal muscle in rats. Pubmed: 23816242 |
PLoS One. | HIF-1α Is Essential for Effective PMN Bacterial Killing, Antimicrobial Peptide Production and Apoptosis in Pseudomonas aeruginosa Keratitis Plos: Source |
Journal of Physiological Anthropology | Telomeres are elongated in rats exposed to moderate altitude Pubmed:Pmc4088304 |
Molecular Medicine Reports | Resveratrol abrogates the effects of hypoxia on cell proliferation, invasion and EMT in osteosarcoma cells through downregulation of the HIF-1α protein. Pubmed:25384583 |
International Journal of Pharmaceutics | The enhancement of gene silencing efficiency with chitosan-coated liposome formulations of siRNAs targeting HIF-1α and VEGF Pubmed:25445537 |
International Journal of Molecular Medicine | Effect of combined treatment with rosuvastatin and protein kinase Cβ2 inhibitor on angiogenesis following myocardial infarction in diabetic rats Pubmed:25524396 |
International Journal of Developmental Neuroscience | Maternal omega-3 fatty acid supplementation to a vitamin B 12 deficient diet normalizes angiogenic markers in the pup brain at birth PubMed: 25889224 |
Eur Surg Res | Determination of the Preferred Conditions for the Isolated Perfusion of Porcine Kidneys PubMed: 25341590 |
Sci Rep | Oxygen-induced cell migration and on-line monitoring biomarkers modulation of cervical cancers on a microfluidic system PubMed: 25905434 |
Evidence-Based Complementary and Alternative Medicine | Metabolomic Analysis of Biochemical Changes in the Plasma of High-Fat Diet and Streptozotocin-Induced Diabetic Rats after Treatment with Isoflavones Extract of Radix Puerariae pmc:PMC4794592 |
Biol Trace Elem Res. | Roles of Copper in Hepatocarcinogenesis via the Activation of Hypoxia-Inducible Factor-1α Pubmed:27121973 |
Раны и раневые инфекции. | Использование гипоксического прекондиционирования для подготовки аутодермотрансплантата у больных со скомпрометированной микроциркуляцией 91 |
Biological Trace Element Research | Roles of Copper in Hepatocarcinogenesis via the Activation of Hypoxia-Inducible Factor-1α. pubmed:27121973 |
Experimental and Therapeutic Medicine | Expression of hypoxia-inducible factor-1α in synovial fluid and articular cartilage is associated with disease severity in knee osteoarthritis etm:13 |
Clinical Trials in Degenerative Diseases | Correlating oxidative stress-related factors with bone metabolic markers in older adult male patients exhibiting degenerative osteoporosis in the high-altitude hypoxic area of China: study protocol for a non-randomized controlled trial issn:2542-3975 |
Annals of Clinical & Laboratory Science | R132H Mutation in IDH1 Gene is Associated with Increased Tumor HIF1-Alpha and Serum VEGF Levels in Primary Glioblastoma Multiforme pubmed:28667042 |
Archives of Gynecology and Obstetrics | Is there any relationship between adipocytokines and angiogenesis factors to address endothelial dysfunction and platelet aggregation in untreated patients with preeclampsia? 10.1007/s00404-017-4461-2 |
EXPERIMENTAL AND THERAPEUTIC MEDICINE | Expression of hypoxia-inducible factor-1α in synovial fluid and articular cartilage is associated with disease severity in knee pubmed:28123469 |
Medical Journal Armed Forces India | Study of acute hypoxia markers in healthy subjects: Utility in post-crash investigation 10.1016/j.mjafi.2017.04.003 |
Journal of Physiological Anthropology | Telomere elongation protects heart and lung tissue cells from fatal damage in rats exposed to severe hypoxia Pubmed:29454386 |
Bulletin of Experimental Biology and Medicine | Signal Mechanism of the Protective Effect of Combined Preconditioning by Amtizole and Moderate Hypoxia Pubmed:29308565 |
Microchimica Acta | Determination of hypoxia-inducible factor-1 by using a ratiometric colorimetric test based on click-mediated growth of gold nanoparticles Pubmed: 30209641 |
Bulletin of Experimental Biology and Medicine | Expression of Hif-1α, Nf-κb, and Vegf Genes in the Liver and Blood Serum Levels of HIF-1α, Erythropoietin, VEGF, TGF-β, 8-Isoprostane, and Corticosterone in Wistar Rats with High and Low Resistance to Hypoxia. Corticosterone in … Pubmed: 30353332 |
Translational Oncology | OKN-007 Increases temozolomide (TMZ) Sensitivity and Suppresses TMZ-Resistant Glioblastoma (GBM) Tumor Growth Pubmed: 30468988 |
West Indian Medical Journal | Plasma Cellular Hypoxia, Mitochondrial Dysfunction, Disease Risk and Prognostic Factors in Type 2 Diabetic Patients in Saudi Arabia. |
Bulletin of Experimental Biology and Medicine | Age-Specific Features of Hypoxia Tolerance and Intensity of Lipopolysaccharide-Induced Systemic Inflammatory Response in Wistar Rats Pubmed: 30903510 |
The role of amniotic fluid exosome, hypoxia inducible fator-1α, and inflammatory cytokine in cervical insufficiency | |
scandinavian journal of immunology | Vitexin alleviates interleukin‐1β induced inflammatory responses in chondrocytes from osteoarthritis patients: involvement of HIF‐1α pathway Pubmed: 31055848 |
JOURNAL OF PERIODONTOLOGY | A disintegrin‐like and metalloproteinase with thrombospondin‐1 (ADAMTS‐1) levels in gingival crevicular fluid correlate with vascular endothelial growth factor‐A … Pubmed: 31020669 |
Tissue Eng Part A | Hypoxia Preconditioned Adipose Derived Endothelial Progenitor Cells Promote Bladder Augmentation Pubmed: 31238789 |
ПОТЕНЦИРОВАНИЕ АНТИГИПОКСАНТАМИ ЭФФЕКТА ГИПОКСИЧЕСКОГО ПРЕКОНДИЦИОНИРОВАНИЯ | |
molecular and cellular biochemistry | Maternal omega-3 fatty acids and vitamin E improve placental angiogenesis in late-onset but not early-onset preeclampsia Pubmed: 31420792 |
Molecules | Regulation of MicroRNA-155 and Its Related Genes Expression by Inositol Hexaphosphate in Colon Cancer Cells Pubmed: 31744065 |
Stem Cell Res Ther | Short-wave enhances mesenchymal stem cell recruitment in fracture healing by increasing HIF-1 in callus Pubmed: 32894200 |
J Cell Physiol | PLCε regulates metabolism and metastasis signaling via HIF‐1α/MEK/ERK pathway in prostate cancer Pubmed: 32383180 |
Гипоксия-зависимый транскрипционный контроль активности деструктивных изменений пародонта воспалительного и злокачественного генеза | |
Food Funct | Prenatal vitamin D supplementation reduces blood pressure and improves placental angiogenesis in an animal model of preeclampsia 33237074 |
Eur J Pharmacol | Lactucaxanthin protects retinal pigment epithelium from hyperglycemia-regulated hypoxia/ER stress/VEGF pathway mediated angiogenesis in ARPE-19 cell and rat ¡ 33705802 |
EXPERIMENTAL EYE RESEARCH | Astaxanthin mediated regulation of VEGF through HIF1¦Á and XBP1 signaling pathway: An insight from ARPE-19 cell and streptozotocin mediated diabetic rat model 33789142 |
scientific reports | Serum hypoxia-inducible factor-1¦Á and uterine artery Doppler ultrasound during the first trimester for prediction of preeclampsia 33758274 |
Molecular Biology Reports | Umbilical cord-derived mesenchymal stem cell conditioned medium reverses neuronal oxidative injury by inhibition of TRPM2 activation and the JNK signaling … Pubmed:35585377 |