Hypoxia Inducible Factor 1 Alpha (HIF1a)
[Edit]HIF1-A; MOP1; PASD8; Basic Helix-Loop-Helix Transcription Factor; ARNT-interacting protein; Basic-helix-loop-helix-PAS protein MOP1; Class E basic helix-loop-helix protein 78
The HIF (hypoxia-inducible factor) transcription factor is a member of the basic-helix-loop-helix (bHLH) family of transcription factors and plays an important role in maintaining cellular oxygen homeostasis. The hypoxia-inducible transcription factor 1α (HIF-1α) is the regulated member of the transcription factor heterodimer HIF-1. HIF-1α has emerged as an important drug target in breast and prostate cancer, cardiovascular disease, and ischemia.
HIF-1alpha abundance (and its subsequent activity) is regulated transcriptionally in an NF-κB dependent manner. In addition, the coordinated activity of the prolyl hydroxylases (PHDs) maintain the appropriate balance of HIF-1α protein in the post-translation phase.
Organism species: Homo sapiens (Human)
Organism species: Mus musculus (Mouse)
CATALOG NO. | PRODUCT NAME | APPLICATIONS | |
Proteins | RPA798Mu01 | Recombinant Hypoxia Inducible Factor 1 Alpha (HIF1a) | Positive Control; Immunogen; SDS-PAGE; WB. |
RPA798Mu02 | Recombinant Hypoxia Inducible Factor 1 Alpha (HIF1a) | Positive Control; Immunogen; SDS-PAGE; WB. | |
Antibodies | PAA798Mu01 | Polyclonal Antibody to Hypoxia Inducible Factor 1 Alpha (HIF1a) | WB; IHC; ICC; IP. |
LAA798Mu71 | Biotin-Linked Polyclonal Antibody to Hypoxia Inducible Factor 1 Alpha (HIF1a) | WB; IHC; ICC. | |
LAA798Mu81 | FITC-Linked Polyclonal Antibody to Hypoxia Inducible Factor 1 Alpha (HIF1a) | WB; IHC; ICC; IF. | |
PAA798Mu02 | Polyclonal Antibody to Hypoxia Inducible Factor 1 Alpha (HIF1a) | WB; IHC; ICC; IP. | |
Assay Kits | SEA798Mu | ELISA Kit for Hypoxia Inducible Factor 1 Alpha (HIF1a) | Enzyme-linked immunosorbent assay for Antigen Detection. |
SCA798Mu | CLIA Kit for Hypoxia Inducible Factor 1 Alpha (HIF1a) | Chemiluminescent immunoassay for Antigen Detection. |
Organism species: Rattus norvegicus (Rat)
CATALOG NO. | PRODUCT NAME | APPLICATIONS | |
Proteins | RPA798Ra01 | Recombinant Hypoxia Inducible Factor 1 Alpha (HIF1a) | Positive Control; Immunogen; SDS-PAGE; WB. |
RPA798Ra02 | Recombinant Hypoxia Inducible Factor 1 Alpha (HIF1a) | Positive Control; Immunogen; SDS-PAGE; WB. | |
Antibodies | PAA798Ra01 | Polyclonal Antibody to Hypoxia Inducible Factor 1 Alpha (HIF1a) | WB; IHC; ICC; IP. |
PAA798Ra02 | Polyclonal Antibody to Hypoxia Inducible Factor 1 Alpha (HIF1a) | WB; IHC; ICC; IP. | |
LAA798Ra71 | Biotin-Linked Polyclonal Antibody to Hypoxia Inducible Factor 1 Alpha (HIF1a) | WB; IHC; ICC. | |
Assay Kits | SEA798Ra | ELISA Kit for Hypoxia Inducible Factor 1 Alpha (HIF1a) | Enzyme-linked immunosorbent assay for Antigen Detection. |
SCA798Ra | CLIA Kit for Hypoxia Inducible Factor 1 Alpha (HIF1a) | Chemiluminescent immunoassay for Antigen Detection. | |
HEA798Ra | High Sensitive ELISA Kit for Hypoxia Inducible Factor 1 Alpha (HIF1a) | Enzyme-linked immunosorbent assay for Antigen Detection. |
Organism species: Oryctolagus cuniculus (Rabbit)
CATALOG NO. | PRODUCT NAME | APPLICATIONS | |
Proteins | RPA798Rb01 | Recombinant Hypoxia Inducible Factor 1 Alpha (HIF1a) | Positive Control; Immunogen; SDS-PAGE; WB. |
Antibodies | PAA798Rb51 | Polyclonal Antibody to Hypoxia Inducible Factor 1 Alpha (HIF1a) | WB; IHC; ICC; IP. |
MAA798Rb21 | Monoclonal Antibody to Hypoxia Inducible Factor 1 Alpha (HIF1a) | WB; IHC; ICC; IP. | |
Assay Kits | SEA798Rb | ELISA Kit for Hypoxia Inducible Factor 1 Alpha (HIF1a) | Enzyme-linked immunosorbent assay for Antigen Detection. |
Organism species: Sus scrofa; Porcine (Pig)
CATALOG NO. | PRODUCT NAME | APPLICATIONS | |
Proteins | RPA798Po01 | Recombinant Hypoxia Inducible Factor 1 Alpha (HIF1a) | Positive Control; Immunogen; SDS-PAGE; WB. |
Antibodies | PAA798Po01 | Polyclonal Antibody to Hypoxia Inducible Factor 1 Alpha (HIF1a) | WB; IHC; ICC; IP. |
MAA798Po21 | Monoclonal Antibody to Hypoxia Inducible Factor 1 Alpha (HIF1a) | WB; IHC; ICC; IP. | |
Assay Kits | SEA798Po | ELISA Kit for Hypoxia Inducible Factor 1 Alpha (HIF1a) | Enzyme-linked immunosorbent assay for Antigen Detection. |
SCA798Po | CLIA Kit for Hypoxia Inducible Factor 1 Alpha (HIF1a) | Chemiluminescent immunoassay for Antigen Detection. |
Organism species: Bos taurus; Bovine (Cattle)
CATALOG NO. | PRODUCT NAME | APPLICATIONS | |
Proteins | RPA798Bo01 | Recombinant Hypoxia Inducible Factor 1 Alpha (HIF1a) | Positive Control; Immunogen; SDS-PAGE; WB. |
RPA798Bo02 | Recombinant Hypoxia Inducible Factor 1 Alpha (HIF1a) | Positive Control; Immunogen; SDS-PAGE; WB. | |
Antibodies | PAA798Bo01 | Polyclonal Antibody to Hypoxia Inducible Factor 1 Alpha (HIF1a) | WB; IHC; ICC; IP. |
LAA798Bo71 | Biotin-Linked Polyclonal Antibody to Hypoxia Inducible Factor 1 Alpha (HIF1a) | WB; IHC; ICC. | |
PAA798Bo02 | Polyclonal Antibody to Hypoxia Inducible Factor 1 Alpha (HIF1a) | WB; IHC; ICC; IP. | |
Assay Kits | n/a | CLIA Kit for Hypoxia Inducible Factor 1 Alpha (HIF1a) | CLIA Kit Customized Service Offer |
n/a | ELISA Kit for Hypoxia Inducible Factor 1 Alpha (HIF1a) | ELISA Kit Customized Service Offer |
Organism species: Chicken (Gallus)
CATALOG NO. | PRODUCT NAME | APPLICATIONS | |
Proteins | RPA798Ga01 | Recombinant Hypoxia Inducible Factor 1 Alpha (HIF1a) | Positive Control; Immunogen; SDS-PAGE; WB. |
RPA798Ga02 | Recombinant Hypoxia Inducible Factor 1 Alpha (HIF1a) | Positive Control; Immunogen; SDS-PAGE; WB. | |
RPA798Ga03 | Recombinant Hypoxia Inducible Factor 1 Alpha (HIF1a) | Positive Control; Immunogen; SDS-PAGE; WB. | |
Antibodies | PAA798Ga01 | Polyclonal Antibody to Hypoxia Inducible Factor 1 Alpha (HIF1a) | WB; IHC; ICC; IP. |
PAA798Ga02 | Polyclonal Antibody to Hypoxia Inducible Factor 1 Alpha (HIF1a) | WB; IHC; ICC; IP. | |
PAA798Ga03 | Polyclonal Antibody to Hypoxia Inducible Factor 1 Alpha (HIF1a) | WB; IHC; ICC; IP. | |
LAA798Ga71 | Biotin-Linked Polyclonal Antibody to Hypoxia Inducible Factor 1 Alpha (HIF1a) | WB; IHC; ICC. | |
Assay Kits | n/a | CLIA Kit for Hypoxia Inducible Factor 1 Alpha (HIF1a) | CLIA Kit Customized Service Offer |
n/a | ELISA Kit for Hypoxia Inducible Factor 1 Alpha (HIF1a) | ELISA Kit Customized Service Offer |
- "Hypoxia-inducible factor 1 is a basic-helix-loop-helix-PAS heterodimer regulated by cellular O2 tension."Proc. Natl. Acad. Sci. U.S.A. 92:5510-5514(1995) [PubMed] [Europe PMC] [Abstract]
- "Characterization of a subset of the basic-helix-loop-helix-PAS superfamily that interacts with components of the dioxin signaling pathway."J. Biol. Chem. 272:8581-8593(1997) [PubMed] [Europe PMC] [Abstract]
- "The human hypoxia-inducible factor 1alpha gene: HIF1A structure and evolutionary conservation."Genomics 52:159-165(1998) [PubMed] [Europe PMC] [Abstract]
- "Preferential expression of the novel alternative isoform I.3 of hypoxia-inducible factor 1alpha in activated human T lymphocytes."Hum. Immunol. 69:421-425(2008) [PubMed] [Europe PMC] [Abstract]
- "The DNA sequence and analysis of human chromosome 14." Nature 421:601-607(2003) [PubMed] [Europe PMC] [Abstract]
- "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
- "An essential role for p300/CBP in the cellular response to hypoxia."Proc. Natl. Acad. Sci. U.S.A. 93:12969-12973(1996) [PubMed] [Europe PMC] [Abstract]
- "Transactivation and inhibitory domains of hypoxia-inducible factor 1alpha. Modulation of transcriptional activity by oxygen tension."J. Biol. Chem. 272:19253-19260(1997) [PubMed] [Europe PMC] [Abstract]
- "Signal transduction in hypoxic cells: inducible nuclear translocation and recruitment of the CBP/p300 coactivator by the hypoxia-inducible factor-1alpha."EMBO J. 17:6573-6586(1998) [PubMed] [Europe PMC] [Abstract]
- "Regulation of hypoxia-inducible factor 1alpha is mediated by an O2-dependent degradation domain via the ubiquitin-proteasome pathway."Proc. Natl. Acad. Sci. U.S.A. 95:7987-7992(1998) [PubMed] [Europe PMC] [Abstract]
- "Molecular mechanisms of transcription activation by HLF and HIF1alpha in response to hypoxia: their stabilization and redox signal-induced interaction with CBP/p300."EMBO J. 18:1905-1914(1999) [PubMed] [Europe PMC] [Abstract]
- "Functional role of p35srj, a novel p300/CBP binding protein, during transactivation by HIF-1."Genes Dev. 13:64-75(1999) [PubMed] [Europe PMC] [Abstract]
- "Drosophila von Hippel-Lindau tumor suppressor complex possesses E3 ubiquitin ligase activity."Biochem. Biophys. Res. Commun. 276:355-361(2000) [PubMed] [Europe PMC] [Abstract]
- "Mechanism of regulation of the hypoxia-inducible factor-1 alpha by the von Hippel-Lindau tumor suppressor protein."EMBO J. 19:4298-4309(2000) [PubMed] [Europe PMC] [Abstract]
- "Redox-regulated recruitment of the transcriptional coactivators CREB-binding protein and SRC-1 to hypoxia-inducible factor 1alpha."Mol. Cell. Biol. 20:402-415(2000) [PubMed] [Europe PMC] [Abstract]
- "Hypoxia-inducible factor 1alpha protein expression is controlled by oxygen-regulated ubiquitination that is disrupted by deletions and missense mutations."Proc. Natl. Acad. Sci. U.S.A. 97:4748-4753(2000) [PubMed] [Europe PMC] [Abstract]
- "Independent function of two destruction domains in hypoxia-inducible factor-alpha chains activated by prolyl hydroxylation."EMBO J. 20:5197-5206(2001) [PubMed] [Europe PMC] [Abstract]
- "Binding and regulation of HIF-1alpha by a subunit of the proteasome complex, PSMA7."FEBS Lett. 498:62-66(2001) [PubMed] [Europe PMC] [Abstract]
- "Targeting of HIF-alpha to the von Hippel-Lindau ubiquitylation complex by O2-regulated prolyl hydroxylation."Science 292:468-472(2001) [PubMed] [Europe PMC] [Abstract]
- "Regulation and destabilization of HIF-1alpha by ARD1-mediated acetylation."Cell 111:709-720(2002) [PubMed] [Europe PMC] [Abstract]
- "FIH-1 is an asparaginyl hydroxylase enzyme that regulates the transcriptional activity of hypoxia-inducible factor."Genes Dev. 16:1466-1471(2002) [PubMed] [Europe PMC] [Abstract]
- "Biochemical purification and pharmacological inhibition of a mammalian prolyl hydroxylase acting on hypoxia-inducible factor."Proc. Natl. Acad. Sci. U.S.A. 99:13459-13464(2002) [PubMed] [Europe PMC] [Abstract]
- "S-nitrosation of Cys-800 of HIF-1alpha protein activates its interaction with p300 and stimulates its transcriptional activity."FEBS Lett. 549:105-109(2003) [PubMed] [Europe PMC] [Abstract]
- "HIF-1 alpha protein as a target for S-nitrosation."FEBS Lett. 535:106-112(2003) [PubMed] [Europe PMC] [Abstract]
- "Structural basis for negative regulation of hypoxia-inducible factor-1alpha by CITED2."Nat. Struct. Biol. 10:504-512(2003) [PubMed] [Europe PMC] [Abstract]
- "Sumoylation increases HIF-1alpha stability and its transcriptional activity."Biochem. Biophys. Res. Commun. 324:394-400(2004) [PubMed] [Europe PMC] [Abstract]
- "Molecular cloning and characterization of the von Hippel-Lindau-like protein."Mol. Cancer Res. 2:43-52(2004) [PubMed] [Europe PMC] [Abstract]
- "VHL protein-interacting deubiquitinating enzyme 2 deubiquitinates and stabilizes HIF-1alpha."EMBO Rep. 6:373-378(2005) [PubMed] [Europe PMC] [Abstract]
- "Interaction between HIF-1 alpha (ODD) and hARD1 does not induce acetylation and destabilization of HIF-1 alpha."FEBS Lett. 579:6428-6432(2005) [PubMed] [Europe PMC] [Abstract]
- "Histone deacetylase inhibitors repress the transactivation potential of hypoxia-inducible factors independently of direct acetylation of HIF-alpha."J. Biol. Chem. 281:13612-13619(2006) [PubMed] [Europe PMC] [Abstract]
- "Clioquinol, a Cu(II)/Zn(II) chelator, inhibits both ubiquitination and asparagine hydroxylation of hypoxia-inducible factor-1alpha, leading to expression of vascular endothelial growth factor and erythropoietin in normoxic cells."J. Biol. Chem. 281:34056-34063(2006) [PubMed] [Europe PMC] [Abstract]
- "SUMOylation of hypoxia-inducible factor-1alpha reduces its transcriptional activity."Biochem. Biophys. Res. Commun. 360:646-652(2007) [PubMed] [Europe PMC] [Abstract]
- "RSUME, a small RWD-containing protein, enhances SUMO conjugation and stabilizes HIF-1alpha during hypoxia."Cell 131:309-323(2007) [PubMed] [Europe PMC] [Abstract]
- "RACK1 competes with HSP90 for binding to HIF-1alpha and is required for O(2)-independent and HSP90 inhibitor-induced degradation of HIF-1alpha."Mol. Cell 25:207-217(2007) [PubMed] [Europe PMC] [Abstract]
- "HIF-1alpha and EPAS ubiquitination mediated by the VHL tumour suppressor involves flexibility in the ubiquitination mechanism, similar to other RING E3 ligases."Oncogene 26:604-609(2007) [PubMed] [Europe PMC] [Abstract]
- "Transcriptional activation of HIF-1 by RORalpha and its role in hypoxia signaling."Arterioscler. Thromb. Vasc. Biol. 28:1796-1802(2008) [PubMed] [Europe PMC] [Abstract]
- "HIF-1 regulation: not so easy come, easy go."Trends Biochem. Sci. 33:526-534(2008) [PubMed] [Europe PMC] [Abstract]
- "Transcriptional regulation of hypoxia-inducible factor 1alpha by HIPK2 suggests a novel mechanism to restrain tumor growth."Biochim. Biophys. Acta 1793:368-377(2009) [PubMed] [Europe PMC] [Abstract]
- "HUMMR, a hypoxia- and HIF-1alpha-inducible protein, alters mitochondrial distribution and transport."J. Cell Biol. 185:1065-1081(2009) [PubMed] [Europe PMC] [Abstract]
- "Hypoxia-inducible protein 2 is a novel lipid droplet protein and a specific target gene of hypoxia-inducible factor-1."FASEB J. 24:4443-4458(2010) [PubMed] [Europe PMC] [Abstract]
- "Plk3 functions as an essential component of the hypoxia regulatory pathway by direct phosphorylation of HIF-1alpha."J. Biol. Chem. 285:38944-38950(2010) [PubMed] [Europe PMC] [Abstract]
- "Casein kinase 1 regulates human hypoxia-inducible factor HIF-1."J. Cell Sci. 123:2976-2986(2010) [PubMed] [Europe PMC] [Abstract]
- "UBXN7 docks on neddylated cullin complexes using its UIM motif and causes HIF1alpha accumulation."BMC Biol. 10:36-36(2012) [PubMed] [Europe PMC] [Abstract]
- "RSUME is implicated in HIF-1-induced VEGF-A production in pituitary tumour cells."Endocr. Relat. Cancer 19:13-27(2012) [PubMed] [Europe PMC] [Abstract]
- "Ubiquitin-specific protease 19 (USP19) regulates hypoxia-inducible factor 1alpha (HIF-1alpha) during hypoxia."J. Biol. Chem. 287:1962-1969(2012) [PubMed] [Europe PMC] [Abstract]
- "The LIMD1 protein bridges an association between the prolyl hydroxylases and VHL to repress HIF-1 activity."Nat. Cell Biol. 14:201-208(2012) [PubMed] [Europe PMC] [Abstract]
- "In silico structural and functional characterization of the RSUME splice variants."PLoS ONE 8:E57795-E57795(2013) [PubMed] [Europe PMC] [Abstract]
- "SIRT2 regulates tumour hypoxia response by promoting HIF-1alpha hydroxylation."Oncogene 34:1354-1362(2015) [PubMed] [Europe PMC] [Abstract]
- "A model for the complex between the hypoxia-inducible factor-1 (HIF-1) and its consensus DNA sequence."J. Biomol. Struct. Dyn. 18:169-179(2000) [PubMed] [Europe PMC] [Abstract]
- "Structure of factor-inhibiting hypoxia-inducible factor (HIF) reveals mechanism of oxidative modification of HIF-1 alpha."J. Biol. Chem. 278:1802-1806(2003) [PubMed] [Europe PMC] [Abstract]
- "Structural basis for recruitment of CBP/p300 by hypoxia-inducible factor-1 alpha."Proc. Natl. Acad. Sci. U.S.A. 99:5367-5372(2002) [PubMed] [Europe PMC] [Abstract]
- "Structural basis for Hif-1 alpha /CBP recognition in the cellular hypoxic response."Proc. Natl. Acad. Sci. U.S.A. 99:5271-5276(2002) [PubMed] [Europe PMC] [Abstract]
- "Structure of an HIF-1alpha-pVHL complex: hydroxyproline recognition in signaling."Science 296:1886-1889(2002) [PubMed] [Europe PMC] [Abstract]
- "Structural basis for the recognition of hydroxyproline in HIF-1 alpha by pVHL."Nature 417:975-978(2002) [PubMed] [Europe PMC] [Abstract]