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Recombinant Neutrophil Elastase (NE)

ELANE; HLE; HNE; ELA2; PMN-E; Neutrophil Elastase; Medullasin; Polymorphonuclear Leukocyte Elastase; Bone Marrow Serine Protease; Leukocyte Elastase; PMN Elastase

  • Product No.RPA181Ca01
  • Organism SpeciesCanis familiaris; Canine (Dog) Same name, Different species.
  • SourceProkaryotic expression
  • HostE.coli
  • Endotoxin Level<1.0EU per 1µg (determined by the LAL method)
  • Subcellular LocationSecreted
  • Predicted Molecular Mass27.04kDa
  • Accurate Molecular Mass25kDa(Analysis of differences refer to the manual)
  • Residues & TagsIle31~Arg248 with N-terminal His Tag
  • Buffer Formulation100mMNaHCO3, 500mMNaCl, pH8.3, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
  • Traits Freeze-dried powder
  • Purity> 95%
  • Isoelectric Point8.6
  • Applications Positive Control; Immunogen; SDS-PAGE; WB.
    If bio-activity of the protein is needed, please check active protein.
  • DownloadInstruction Manual
  • UOM 10µg50µg 200µg 1mg 5mg
  • FOB US$ 164  US$ 410  US$ 820  US$ 2460  US$ 6150 
    For more details, please contact local distributors!

SEQUENCE

USAGE

Reconstitute in ddH2O to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
Mediators of Inflammation Long-Term Leukocyte Filtration Should Be Avoided during Extracorporeal Circulation Hindawi: 612848
Journal of Photochemistry and Photobiology B: Biology A dietary supplement improves facial photoaging and skin sebum, hydration and tonicity modulating serum fibronectin, neutrophil elastase 2, hyaluronic acid and carbonylated proteins Pubmed:25732262
International Journal of Molecular Medicine The neutrophil elastase inhibitor, sivelestat, attenuates sepsis-related kidney injury in rats pubmed:27430552
European Journal of Trauma and Emergency Xuebijing injection treatment inhibits vasopermeability and reduces fluid requirements in acanine burn model. pubmed:28070608
Clin Exp Pharmacol Physiol. Inhibition of ALDH2 protects PC12 cells against formaldehyde-induced cytotoxicity: involving the protection of hydrogen sulphide. pubmed:28251688
Journal of Cardiology Targeted regulation of sympathetic activity in paraventricular nucleus reduces inducible ventricular arrhythmias in rats after myocardial infarction 10.1016:j.jjcc.2018.06.003
Neuroscience Angiotensin II Type 1a Receptors in the Subfornical Organ Modulate Neuroinflammation in the Hypothalamic Paraventricular Nucleus in Heart Failure Rats Pubmed:29684507
Molecular and Cellular Biochemistry Repetitive transcranial magnetic stimulation inhibits Sirt1/MAO-A signaling in the prefrontal cortex in a rat model of depression and cortex-derived astrocytes Pubmed:28948423
Neuropsychiatric disease and treatment The depressive-like behaviors of chronic unpredictable mild stress-treated mice, ameliorated by Tibetan medicine Zuotai: involvement in the hypothalamic … Pubmed:29379286
Behavioural Brain Research Possible mechanisms of prenatal cold stress induced-anxiety-like behavior depression in offspring rats Pubmed: 30423388
Journal of Pineal Research Role of melatonin in sleep deprivation‐induced intestinal barrier dysfunction in mice Pubmed: 30929267
Fundamental & clinical pharmacology Effect of antimicrobial peptides HNP‐1 and hBD‐1 on Staphylococcus aureus strains in vitro and in vivo Pubmed: 31313350
Cell Death & Disease Erythromycin suppresses neutrophil extracellular traps in smoking-related chronic pulmonary inflammation Pubmed: 31515489
Journal of nutritional science and vitaminology Co-Administration of Curcumin and Artepillin C Induces Development of Brown-Like Adipocytes in Association with Local Norepinephrine Production by Alternatively … Pubmed: 31474682
Journal of Clinical Immunology MiR-608 Exerts Anti-inflammatory Effects by Targeting ELANE in Monocytes Pubmed: 31749032
Pathology & Oncology Research Neutrophil Extracellular Traps Associate with Clinical Stages in Breast Cancer Pubmed: 31656990
Bioscience Reports Inhibition of microRNA-146a attenuated heart failure in myocardial infarction rats Pubmed: 31763669
Oxidative Medicine and Cellular Longevity Neutrophil Elastase Inhibitors Suppress Oxidative Stress in Lung during Liver Transplantation Pubmed: 31871555
Cytokine Levels of pro-and anti-inflammatory cytokines in cystic fibrosis patients with or without gingivitis Pubmed: 31927460
Journal of neural transmission (Vienna, Austria : 1996) Neurotransmitter system aberrations in patients with drug addiction Pubmed: 32804296
experimental eye research Corneal epithelial injury-induced norepinephrine promotes Pseudomonas aeruginosa keratitis Pubmed: 32376471
CLINICAL AND EXPERIMENTAL HYPERTENSION Hyperinsulinemia rather than insulin resistance itself induces blood pressure elevation in high fat diet-fed rats Pubmed: 32349626
Br J Pharmacol Macrophage Extracellular Traps Aggravate Iron Overload©\Related Liver Ischemia/Reperfusion Injury 33959955
Evidence-Based Complementary and Alternative Medicine Zisheng Shenqi Decoction Ameliorates Monosodium Urate-Mediated Gouty Arthritis in Rats via Promotion of Autophagy through the AMPK/mTOR Signaling?¡­ 33505502
Nat Sci Sleep Depression of Non-Neuronal Cholinergic System May Play a Role in Co-Occurrence of Subjective Daytime Sleepiness and Hypertension in Patients with … 34934375
Neuromodulation: Technology at the Neural Interface Transcutaneous Electrical Acustimulation Improved the Quality of Life in Patients With Diarrhea-Irritable Bowel Syndrome Pubmed:35088760
A Novel Role of Tinospora Cordifolia in Amelioration of Cancer-Induced Systemic Deterioration By Taming Neutrophil Infiltration and Hyperactivation
Catalog No. Related products for research use of Canis familiaris; Canine (Dog) Organism species Applications (RESEARCH USE ONLY!)
RPA181Ca01 Recombinant Neutrophil Elastase (NE) Positive Control; Immunogen; SDS-PAGE; WB.
PAA181Ca01 Polyclonal Antibody to Neutrophil Elastase (NE) WB; IHC
MAA181Ca21 Monoclonal Antibody to Neutrophil Elastase (NE) WB; IHC; ICC; IP.
SEA181Ca ELISA Kit for Neutrophil Elastase (NE) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA181Ca Multiplex Assay Kit for Neutrophil Elastase (NE) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.