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Recombinant Leukemia Inhibitory Factor (LIF)

CDF; D-FACTOR; HILDA; MLPLI; Cholinergic Differentiation Factor; Differentiation-Stimulating Factor; Melanoma-Derived LPL Inhibitor; Emfilermin

  • Product No.RPA085Ra01
  • Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
  • SourceProkaryotic expression
  • HostE.coli
  • Endotoxin Level<1.0EU per 1µg (determined by the LAL method)
  • Subcellular LocationSecreted
  • Predicted Molecular Mass23.3kDa
  • Accurate Molecular Mass25kDa(Analysis of differences refer to the manual)
  • Residues & TagsLeu25~Phe202 with N-terminal His Tag
  • Buffer Formulation20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
  • Traits Freeze-dried powder
  • Purity> 95%
  • Isoelectric Point8.9
  • Applications Positive Control; Immunogen; SDS-PAGE; WB.
    If bio-activity of the protein is needed, please check active protein.
  • DownloadInstruction Manual
  • UOM 10µg50µg 200µg 1mg 5mg
  • FOB US$ 140  US$ 350  US$ 700  US$ 2100  US$ 5250 
    For more details, please contact local distributors!

SEQUENCE

USAGE

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
British Journal of Anaesthesia Leukemia inhibitory factor (LIF) potentiates antinociception activity and inhibits tolerance induction of opioids pubmed:28077540
Biological Rhythm Research A study of altered cytokine rhythms associated with successful implantation in cows 10.1080/09291016.2017.1361159
Integrated analysis of cancer stem cells-associated lncRNA-miRNA-mRNA network for ovarian cancer via microarray and Gene Expression Omnibus database
Catalog No. Related products for research use of Rattus norvegicus (Rat) Organism species Applications (RESEARCH USE ONLY!)
RPA085Ra01 Recombinant Leukemia Inhibitory Factor (LIF) Positive Control; Immunogen; SDS-PAGE; WB.
APA085Ra01 Active Leukemia Inhibitory Factor (LIF) Cell culture; Activity Assays.
PAA085Ra01 Polyclonal Antibody to Leukemia Inhibitory Factor (LIF) WB; IHC; ICC; IP.
MAA085Ra21 Monoclonal Antibody to Leukemia Inhibitory Factor (LIF) WB; IHC; ICC; IP.
SEA085Ra ELISA Kit for Leukemia Inhibitory Factor (LIF) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA085Ra Multiplex Assay Kit for Leukemia Inhibitory Factor (LIF) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.