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Active Leukemia Inhibitory Factor (LIF)

CDF; D-FACTOR; HILDA; MLPLI; Cholinergic Differentiation Factor; Differentiation-Stimulating Factor; Melanoma-Derived LPL Inhibitor; Emfilermin

ACTIVITY TEST

Leukemia inhibitory factor (LIF), is an interleukin 6 class cytokine that affects cell growth by inhibiting differentiation. LIF as a cytokine also has another fuction including: the growth promotion and cell differentiation of different types of target cells, influence on bone metabolism, cachexia, neural development, embryogenesis and inflammation. To evaluate the activty of recombinant rat LIF, the K562 cells were were seeded into triplicate wells of 96-well plates at a density of 5,000 cells/well with5% serum standard 1640  which contains various concentrations of recombinant rat LIF. After incubated for 72h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10 µl of CCK-8 solution was added to each well of the plate, then the absorbance at 450 nm was measured using a microplate reader after incubating the plate for 2-4 hours at 37 ℃. After incubation with LIF for72h, proliferative inhibition of K562 cells observed by inverted microscope was shown in Figure1. Cell viability was assessed by CCK-8(Cell Counting Kit-8 ) assay after incubation with recombinant LIF for72h. The result was shown in Figure2. It was obvious that LIF significantly inhibit cell viability of K562 cells. The ED50 is 1.44μg/ml.

Figure2. Proliferative inhibition of K562 cells after stimulated with LIF.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
British Journal of Anaesthesia Leukemia inhibitory factor (LIF) potentiates antinociception activity and inhibits tolerance induction of opioids pubmed:28077540
Biological Rhythm Research A study of altered cytokine rhythms associated with successful implantation in cows 10.1080/09291016.2017.1361159
Integrated analysis of cancer stem cells-associated lncRNA-miRNA-mRNA network for ovarian cancer via microarray and Gene Expression Omnibus database
Catalog No. Related products for research use of Rattus norvegicus (Rat) Organism species Applications (RESEARCH USE ONLY!)
RPA085Ra01 Recombinant Leukemia Inhibitory Factor (LIF) Positive Control; Immunogen; SDS-PAGE; WB.
APA085Ra01 Active Leukemia Inhibitory Factor (LIF) Cell culture; Activity Assays.
PAA085Ra01 Polyclonal Antibody to Leukemia Inhibitory Factor (LIF) WB; IHC; ICC; IP.
MAA085Ra21 Monoclonal Antibody to Leukemia Inhibitory Factor (LIF) WB; IHC; ICC; IP.
SEA085Ra ELISA Kit for Leukemia Inhibitory Factor (LIF) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA085Ra Multiplex Assay Kit for Leukemia Inhibitory Factor (LIF) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.