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ELISA Kit DIY Materials for Wingless Type MMTV Integration Site Family, Member 3A (WNT3A)
- Product No.KSP155Ra01
- Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
- Detectable SampleTissue homogenates, cell lysates, cell culture supernates and other biological fluids.
- Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
- Detectable Range0.156-10ng/mL
- Applicable Sensitivity0.061ng/mL
- ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
- Downloadn/a
- UOM 96T*596T*10 96T*20 96T*50 96T*100
- FOB
US$ 1368
US$ 2280
US$ 4104
US$ 7980
US$ 13680
For more details, please contact local distributors!
SPECIFITY
The Abs in the kit have high sensitivity and excellent specificity for detection of Wingless Type MMTV Integration Site Family, Member 3A (WNT3A). No significant cross-reactivity or interference between Wingless Type MMTV Integration Site Family, Member 3A (WNT3A) and analogues was observed.
USAGE
1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
STORAGE
Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.
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| J Histochem Cytochem | Wnt3a Mediates the Inhibitory Effect of Hyperoxia on the Transdifferentiation of AECIIs to AECIs PubMed: 26209081 |
| PloS one | Gelatin device for the delivery of growth factors involved in endochondral ossification pubmed:28380024 |
| Life Sciences | Hepatoprotective effect of hesperidin in hepatocellular carcinoma: Involvement of Wnt signaling pathways. pubmed:28754618 |
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| The Showa University Journal of Medical Sciences | Eribulin Treatment Induces High Expression of miR-195 and Inactivates the Wnt/β-catenin Signaling Pathway in Triple-negative Breast Cancer Doi: 10.15369/sujms.30.359 |
| Journal of Cancer | Oncogenic Wnt3a: A Candidate Specific Marker and Novel Molecular Target for Hepatocellular Carcinoma Pubmed: 31737122 |
| CANCER LETTERS | Knock down of BMSC-derived Wnt3a or its antagonist analogs attenuate colorectal carcinogenesis induced by chronic Fusobacterium nucleatum infection Pubmed: 32920199 |
| Biomaterials | An improved osseointegration of metal implants by pitavastatin loaded multilayer films with osteogenic and angiogenic properties 34823885 |