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ELISA Kit DIY Materials for Notch Homolog 3 (NOTCH3)

CADASIL; CASIL; Neurogenic locus notch homolog protein 3

  • Product No.KSL147Hu01
  • Organism SpeciesHomo sapiens (Human) Same name, Different species.
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleTissue homogenates, cell lysates, cell culture supernates and other biological fluids
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range0.156-10ng/mL
  • Applicable Sensitivity0.061ng/mL
  • ApplicationsMain materials for "Do It(ELISA Kit) Yourself"
  • Downloadn/a
  • UOM 96T*596T*10 96T*20 96T*50 96T*100
  • FOB US$ 1386  US$ 2310  US$ 4158  US$ 8085  US$ 13860 
    For more details, please contact local distributors!

SPECIFITY

The Abs in the kit have high sensitivity and excellent specificity for detection of Notch Homolog 3 (NOTCH3). No significant cross-reactivity or interference between Notch Homolog 3 (NOTCH3) and analogues was observed.

USAGE

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

STORAGE

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Magazine Citations
J Assist Reprod Genet GRN, NOTCH3, FN1, and PINK1 expression in eutopic endometrium–potential biomarkers in the detection of endometriosis–a pilot study Pubmed: 33029756
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPL147Hu01 Recombinant Notch Homolog 3 (NOTCH3) Positive Control; Immunogen; SDS-PAGE; WB.
PAL147Hu01 Polyclonal Antibody to Notch Homolog 3 (NOTCH3) WB; IHC; ICC; IP.
SEL147Hu ELISA Kit for Notch Homolog 3 (NOTCH3) Enzyme-linked immunosorbent assay for Antigen Detection.
LML147Hu Multiplex Assay Kit for Notch Homolog 3 (NOTCH3) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
KSL147Hu01 ELISA Kit DIY Materials for Notch Homolog 3 (NOTCH3) Main materials for "Do It(ELISA Kit) Yourself"