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ELISA Kit DIY Materials for Sirtuin 5 (SIRT5)

SIR2L5; Silent Mating Type Information Regulation 2 Homolog 5; NAD-dependent protein deacylase sirtuin-5, mitochondrial; Regulatory protein SIR2 homolog 5; SIR2-like protein 5

  • Product No.KSE915Mu01
  • Organism SpeciesMus musculus (Mouse) Same name, Different species.
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleTissue homogenates and other biological fluids.
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range0.156-10ng/mL
  • Applicable Sensitivity0.067ng/mL
  • ApplicationsMain materials for "Do It(ELISA Kit) Yourself"
  • Downloadn/a
  • UOM 96T*596T*10 96T*20 96T*50 96T*100
  • FOB US$ 1185  US$ 1975  US$ 3555  US$ 6913  US$ 11850 
    For more details, please contact local distributors!

SPECIFITY

The Abs in the kit have high sensitivity and excellent specificity for detection of Sirtuin 5 (SIRT5). No significant cross-reactivity or interference between Sirtuin 5 (SIRT5) and analogues was observed.

USAGE

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

STORAGE

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Magazine Citations
Elife SIRT2 deacetylase regulates the activity of GSK3 isoforms independent of inhibitory phosphorylation Pubmed:29504933
Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
RPE915Mu01 Recombinant Sirtuin 5 (SIRT5) Positive Control; Immunogen; SDS-PAGE; WB.
PAE915Mu01 Polyclonal Antibody to Sirtuin 5 (SIRT5) WB; IHC; ICC; IP.
LAE915Mu71 Biotin-Linked Polyclonal Antibody to Sirtuin 5 (SIRT5) WB; IHC; ICC.
KSE915Mu01 ELISA Kit DIY Materials for Sirtuin 5 (SIRT5) Main materials for "Do It(ELISA Kit) Yourself"