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ELISA Kit DIY Materials for Endothelial Cell Adhesion Molecule (ESAM)

  • Product No.KSE536Mu01
  • Organism SpeciesMus musculus (Mouse) Same name, Different species.
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleTissue homogenates, cell lysates and other biological fluids.
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range0.312-20ng/mL
  • Applicable Sensitivity0.114ng/mL
  • ApplicationsMain materials for "Do It(ELISA Kit) Yourself"
  • Downloadn/a
  • UOM 96T*596T*10 96T*20 96T*50 96T*100
  • FOB US$ 1221  US$ 2035  US$ 3663  US$ 7123  US$ 12210 
    For more details, please contact local distributors!

SPECIFITY

The Abs in the kit have high sensitivity and excellent specificity for detection of Endothelial Cell Adhesion Molecule (ESAM). No significant cross-reactivity or interference between Endothelial Cell Adhesion Molecule (ESAM) and analogues was observed.

USAGE

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

STORAGE

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Magazine Citations
Microvascular Research Endothelial cell-selective adhesion molecule regulates albuminuria in diabetic nephropathy Wiley: Source
Scand J Clin Lab Invest. Relationship of endothelial cell selective adhesion molecule to markers of oxidative stress in type 2 diabetes Pubmed:24456423
Biomarkers ESAM predicts cardiovascular mortality in diabetic hemodialysis patients PubMed: 26329529
International Urology and Nephrology Adiponectin is related to markers of endothelial dysfunction and neoangiogenesis in diabetic patients Pubmed:29804241
journal of alzheimers disease S-Adenosylmethionine Alleviates Amyloid-β-Induced Neural Injury by Enhancing Trans-Sulfuration Pathway Activity in Astrocytes Pubmed: 32597804
Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
RPE536Mu01 Recombinant Endothelial Cell Adhesion Molecule (ESAM) Positive Control; Immunogen; SDS-PAGE; WB.
PAE536Mu01 Polyclonal Antibody to Endothelial Cell Adhesion Molecule (ESAM) WB; IHC; ICC; IP.
LAE536Mu81 FITC-Linked Polyclonal Antibody to Endothelial Cell Adhesion Molecule (ESAM) WB; IHC; ICC; IF.
LAE536Mu71 Biotin-Linked Polyclonal Antibody to Endothelial Cell Adhesion Molecule (ESAM) WB; IHC; ICC.
KSE536Mu01 ELISA Kit DIY Materials for Endothelial Cell Adhesion Molecule (ESAM) Main materials for "Do It(ELISA Kit) Yourself"