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ELISA Kit DIY Materials for Surfactant Associated Protein A2 (SPA2)

SFTPA2B; COLEC5; SP2A; SPA1 beta; SP-A2; SPAII; Collectin-5; Pulmonary Surfactant Protein A2; 35 kDa pulmonary surfactant-associated protein; Alveolar proteinosis protein

  • Product No.KSD521Ra01
  • Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleTissue homogenates, cell lysates, cell culture supernates and other biological fluids.
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range0.156-10ng/mL
  • Applicable Sensitivity0.070ng/mL
  • ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
  • Downloadn/a
  • UOM 96T*596T*10 96T*20 96T*50 96T*100
  • FOB US$ 1288  US$ 2146  US$ 3863  US$ 7511  US$ 12876 
    For more details, please contact local distributors!

SPECIFITY

The Abs in the kit have high sensitivity and excellent specificity for detection of Surfactant Associated Protein A2 (SPA2). No significant cross-reactivity or interference between Surfactant Associated Protein A2 (SPA2) and analogues was observed.

USAGE

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

STORAGE

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Magazine Citations
Respiratory research Effects and molecular mechanisms of intrauterine infection/inflammation on lung development Pubmed:29747649
Lung  Serum Surfactant Protein Levels in Patients Admitted to the Hospital with Acute COPD Exacerbation Pubmed:29445934
Catalog No. Related products for research use of Rattus norvegicus (Rat) Organism species Applications (RESEARCH USE ONLY!)
RPD521Ra01 Recombinant Surfactant Associated Protein A2 (SPA2) Positive Control; Immunogen; SDS-PAGE; WB.
PAD521Ra01 Polyclonal Antibody to Surfactant Associated Protein A2 (SPA2) WB; IHC; ICC; IP.
SED521Ra ELISA Kit for Surfactant Associated Protein A2 (SPA2) Enzyme-linked immunosorbent assay for Antigen Detection.
LMD521Ra Multiplex Assay Kit for Surfactant Associated Protein A2 (SPA2) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
KSD521Ra01 ELISA Kit DIY Materials for Surfactant Associated Protein A2 (SPA2) Main materials for "Do It (ELISA Kit) Yourself".