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ELISA Kit DIY Materials for Apelin (APLN)

XNPEP2; APEL; APJ endogenous ligand

  • Product No.KSD066Ra11
  • Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
  • Reagent Contents Capture Antibody, Biotin-labeled Competitor, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
  • Applicable PrincipleCompetitive Inhibition ELISA for Antigen Detection
  • Detectable Range12.35-1000pg/mL
  • Applicable Sensitivity4.84pg/mL
  • ApplicationsMain materials for "Do It(ELISA Kit) Yourself"
  • Downloadn/a
  • UOM 96T*596T*10 96T*20 96T*50 96T*100
  • FOB US$ 1368  US$ 2280  US$ 4104  US$ 7980  US$ 13680 
    For more details, please contact local distributors!

SPECIFITY

The Abs in the kit have high sensitivity and excellent specificity for detection of Apelin (APLN). No significant cross-reactivity or interference between Apelin (APLN) and analogues was observed.

USAGE

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

STORAGE

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Magazine Citations
Clin Exp Hypertens. Individual and Concomitant Effects of Cardioprotective Programs on Cardiac Apelinergic System and Oxidative State in └-NAME-Induced Hypertension Informahealthcare: Source
African Journal of Pharmacy and Pharmacology Cardioprotective effects of aerobic regular exercise against doxorubicin-induced oxidative stress in rat. Academicjournals: Source
Zahedan Journal of research in Medical Sciences Improvement of Kidney Apelin and Apelin Receptor in Nitro-L-Arginine-Methyl Ester Induced Hypertension Rats Zjrms: Source
Endocr J. CTRP3 modulates the expression and secretion of adipokines in 3T3-L1 adipocytes Pubmed:25168658
Mol Metab TIMP3 interplays with apelin to regulate cardiovascular metabolism in hypercholesterolemic mice PubMed: 26500845
Journal of medicinal food Regulation of GIP and Ghrelin in Healthy Subjects Fed on Sun-Dried Raisins: A Pilot Study with a Crossover Trial Design. pubmed:28170279
Arch Physiol Biochem Associations of apelin, leptin, irisin, ghrelin, insulin, glucose levels, and lipid parameters with physical activity during eight weeks of regular exercise training Pubmed: 31290696
preprints Serum Apelin and Galactin-3 in Preeclampsia in Iraq Pubmed: 32538210
Journal of Diabetes Research The Potential Role of Chemerin, Lipocalin 2, and Apelin in the Diagnosis and Pathophysiology of Gestational Diabetes Mellitus
Catalog No. Related products for research use of Rattus norvegicus (Rat) Organism species Applications (RESEARCH USE ONLY!)
RPD066Ra01 Recombinant Apelin (APLN) Positive Control; Immunogen; SDS-PAGE; WB.
PAD066Ra01 Polyclonal Antibody to Apelin (APLN) IHC
LAD066Ra71 Biotin-Linked Polyclonal Antibody to Apelin (APLN) IHC
MAD066Ra21 Monoclonal Antibody to Apelin (APLN) WB; IHC; ICC; IP.
SED066Ra ELISA Kit for Apelin (APLN) Enzyme-linked immunosorbent assay for Antigen Detection.
CED066Ra ELISA Kit for Apelin (APLN) Enzyme-linked immunosorbent assay for Antigen Detection.
LMD066Ra Multiplex Assay Kit for Apelin (APLN) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
KSD066Ra11 ELISA Kit DIY Materials for Apelin (APLN) Main materials for "Do It(ELISA Kit) Yourself"