ELISA Kit DIY Materials for Leucine Rich Alpha-2-Glycoprotein 1 (LRG1)

  • Product No.KSB934Hu01
  • Organism SpeciesHomo sapiens (Human) Same name, Different species.
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable Sampleserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range3.12-200ng/mL
  • Applicable Sensitivity1.37ng/mL
  • ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
  • Downloadn/a
  • UOM 96T*596T*10 96T*20 96T*50 96T*100
  • FOB US$ 1197  US$ 1995  US$ 3591  US$ 6983  US$ 11970 
    For more details, please contact local distributors!

SPECIFITY

The Abs in the kit have high sensitivity and excellent specificity for detection of Leucine Rich Alpha-2-Glycoprotein 1 (LRG1).No significant cross-reactivity or interference between Leucine Rich Alpha-2-Glycoprotein 1 (LRG1) and analogues was observed.

USAGE

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

STORAGE

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Magazine Citations
Clin Exp Nephrol Differentially expressed urinary biomarkers in children with idiopathic nephrotic syndrome PubMed: 26351173
Journal of Proteome Research Targeted proteomics predict a sustained complete-response after transarterial chemoembolization and clinical outcomes in patients with hepatocellular carcinoma: a prospective cohort study pubmed:28112944
OncoTargets and Therapy leucine-rich alpha-2-glycoprotein-1 is up-regulated in colorectal cancer and is a tumor promoter Pubmed:29785123
PROTEOMICS – Clinical Applications Screening and Identification of Pregnancy Zone Protein (PZP) and Leucine‐Rich Alpha‐2‐Glycoprotein (LRG) as Potential Serum Biomarkers for Early‐onset … Pubmed: 30411527
Cancer Management and Research iTRAQ-based quantitative protein expression profiling of biomarkers in childhood B-cell and T-cell acute lymphoblastic leukemia Pubmed: 31440093
J Clin Med A Three-Protein Panel to Support the Diagnosis of Sepsis in Children Pubmed:35329889
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