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ELISA Kit DIY Materials for Ribonuclease A3 (RNASE3)

Rnase-A3; RnaseA3; ECP; RNS3; Ribonuclease,RNase A Family 3; Eosinophil Cationic Protein

  • Product No.KSB758Ra01
  • Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleSerum, plasma, tissue homogenates and other biological fluids.
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range62.50-4000pg/mL
  • Applicable Sensitivity28.1pg/mL
  • ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
  • Downloadn/a
  • UOM 96T*596T*10 96T*20 96T*50 96T*100
  • FOB US$ 1270  US$ 2117  US$ 3811  US$ 7410  US$ 12702 
    For more details, please contact local distributors!

SPECIFITY

The Abs in the kit have high sensitivity and excellent specificity for detection of Ribonuclease A3 (RNASE3). No significant cross-reactivity or interference between Ribonuclease A3 (RNASE3) and analogues was observed.

USAGE

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

STORAGE

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Magazine Citations
Journal of American Science Assessment of the Role of Serum and Urine Eosinophil Cationic Protein in Diagnosis of Wuchereria bancrofti Infection Jofamerican: source
Molecular Medicine Reports Intercellular adhesion molecule?1 expression in activated eosinophils is associated with mucosal remodeling in nasal polyps Pubmed:25573100
International Journal of Molecular Sciences The Human Host Defense Ribonucleases 1, 3 and 7 Are Elevated in Patients with Sepsis after Major Surgery—A Pilot Study Pubmed:26927088
annals of allergy asthma & immunology Interleukin 16 and CCL17/thymus and activation-regulated chemokine in patients with aspirin-exacerbated respiratory disease. pubmed:27986411
European Journal of Clinical Investigation Dying blood mononuclear cell secretome exerts antimicrobial activity. pubmed:27513763
Analytica Chimica Acta An ultrasensitive electrochemical detection of tryptase using 3D macroporous reduced graphene oxide nanocomposites by one-pot electrochemical synthesis
LARYNGOSCOPE Therapeutic Effects of Intranasal Tofacitinib on Chronic Rhinosinusitis with Nasal Polyps in Mice Pubmed: 33014124
INTERNATIONAL ARCHIVES OF ALLERGY AND IMMUNOLOGY Elevated Serum Level of CD48 in Patients with Intermittent Allergic Rhinitis Pubmed: 32966985
Immunopharmacol Immunotoxicol sCD48 is elevated in non-allergic but not in allergic persistent rhinitis 34477021
Mediators Inflamm Roles Played by the PI3K/Akt/HIF-1α Pathway and IL-17A in the Chinese Subtype of Chronic Sinusitis with Nasal Polyps Pubmed:35075349
Catalog No. Related products for research use of Rattus norvegicus (Rat) Organism species Applications (RESEARCH USE ONLY!)
RPB758Ra01 Recombinant Ribonuclease A3 (RNASE3) Positive Control; Immunogen; SDS-PAGE; WB.
PAB758Ra01 Polyclonal Antibody to Ribonuclease A3 (RNASE3) WB; IHC; ICC; IP.
MAB758Ra21 Monoclonal Antibody to Ribonuclease A3 (RNASE3) WB; IHC; ICC; IP.
LAB758Ra72 Biotin-Linked Monoclonal Antibody to Ribonuclease A3 (RNASE3) WB; IHC; ICC.
PSB758Ra01 Antibody Pair for Ribonuclease A3 (RNASE3) ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays.
KSB758Ra01 ELISA Kit DIY Materials for Ribonuclease A3 (RNASE3) Main materials for "Do It (ELISA Kit) Yourself".