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ELISA Kit DIY Materials for Alpha-1-Antitrypsin (a1AT)

SERPINA1; SPAAT; A1-AT; PI; Serpin Peptidase Inhibitor,Clade A(Alpha-1 Antiproteinase/AntiTrypsin)Member 1; Alpha-1 protease inhibitor; Short peptide from AAT; Serpin A1

  • Product No.KSB697Mu01
  • Organism SpeciesMus musculus (Mouse) Same name, Different species.
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable Sampleserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range31.2-2000ng/mL
  • Applicable Sensitivity12.8ng/mL
  • ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
  • Downloadn/a
  • UOM 96T*596T*10 96T*20 96T*50 96T*100
  • FOB US$ 1231  US$ 2052  US$ 3694  US$ 7182  US$ 12312 
    For more details, please contact local distributors!

SPECIFITY

The Abs in the kit have high sensitivity and excellent specificity for detection of Alpha-1-Antitrypsin (a1AT). No significant cross-reactivity or interference between Alpha-1-Antitrypsin (a1AT) and analogues was observed.

USAGE

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

STORAGE

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Magazine Citations
Journal of Biotechnology Expression and characterization of recombinant human alpha-antitrypsin in transgenic rice seed PubMed: 23376844
Sci Rep Hepatic steatosis depresses alpha-1-antitrypsin levels in human and rat acute pancreatitis PubMed: 26634430
PLoS One Gut Microbial Dysbiosis May Predict Diarrhea and Fatigue in Patients Undergoing Pelvic Cancer Radiotherapy: A Pilot Study PubMed: 25955845
Respir Care Diagnostic Utility of Biomarkers in COPD PubMed: 26106205
Acta Anaesthesiologica Scandinavica Early prognostic factors in septic shock cancer patients: a prospective study with a proteomic approach Pubmed:29315472
Journal of Clinical Medicine Urinary Proteomics for the Early Diagnosis of Diabetic Nephropathy in Taiwanese Patients Pubmed: 30486327
Journal of Proteomics Salivary proteins from dysplastic leukoplakia and oral squamous cell carcinoma and their potential for early detection Pubmed: 31706945
Diagnostics Systemic Alterations of Immune Response-Related Proteins during Glaucoma Development in the Murine Model DBA/2J Pubmed: 32585848
Pediatrics International Prenatal diagnosis of maternal serum from mothers carrying β‐thalassemic fetus 34559910
Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
RPB697Mu02 Recombinant Alpha-1-Antitrypsin (a1AT) Positive Control; Immunogen; SDS-PAGE; WB.
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PAB697Mu01 Polyclonal Antibody to Alpha-1-Antitrypsin (a1AT) WB,IHC
PAB697Mu02 Polyclonal Antibody to Alpha-1-Antitrypsin (a1AT) WB; IHC; ICC; IP.
LAB697Mu81 FITC-Linked Polyclonal Antibody to Alpha-1-Antitrypsin (a1AT) WB; IHC; ICC; IF.
LAB697Mu71 Biotin-Linked Polyclonal Antibody to Alpha-1-Antitrypsin (a1AT) WB; IHC; ICC.
SEB697Mu ELISA Kit for Alpha-1-Antitrypsin (a1AT) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB697Mu Multiplex Assay Kit for Alpha-1-Antitrypsin (a1AT) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
KSB697Mu01 ELISA Kit DIY Materials for Alpha-1-Antitrypsin (a1AT) Main materials for "Do It (ELISA Kit) Yourself".