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ELISA Kit DIY Materials for Trefoil Factor 3 (TFF3)

HITF; ITF3; TFI; HP1.B; Trefoil Factor 3, Intestinal

  • Product No.KSB656Hu01
  • Organism SpeciesHomo sapiens (Human) Same name, Different species.
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range62.5-4000pg/mL
  • Applicable Sensitivity23.8pg/mL
  • ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
  • Downloadn/a
  • UOM 96T*596T*10 96T*20 96T*50 96T*100
  • FOB US$ 896  US$ 1494  US$ 2689  US$ 5229  US$ 8964 
    For more details, please contact local distributors!

SPECIFITY

The Abs in the kit have high sensitivity and excellent specificity for detection of Trefoil Factor 3, Intestinal (TFF3). No significant cross-reactivity or interference between Trefoil Factor 3, Intestinal (TFF3) and analogues was observed.

USAGE

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

STORAGE

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Magazine Citations
BMC Gastroenterology Serum trefoil factor 3 is a promising non-invasive biomarker for gastric cancer screening: A monocentric cohort study in China Biomedcentral: Source
Technology in Cancer Research & Treatment Serum Trefoil Factor 3 as a Protein Biomarker for the Diagnosis of Colorectal Cancer. pubmed:27760866
PLoS One Obstructive sleep apnea and rhonchopathy are associated with downregulation of trefoil factor family peptide 3 (TFF3)-Implications of changes in oral mucus composition. pubmed:29028798
Microbial Pathogenesis Protective effects of sinomenine against LPS-induced inflammation in piglets. pubmed:28757275
Scientific Reports Nucleotide-mediated SPDEF modulates TFF3-mediated wound healing and intestinal barrier function during the weaning process Pubmed:29555969
Scientific Reports Kidney-based in vivo model for drug-induced nephrotoxicity testing Pubmed: 32796873
International Journal of Molecular Sciences Human Synovia Contains Trefoil Factor Family (TFF) Peptides 1–3 Although Synovial Membrane Only Produces TFF3: Implications in Osteoarthritis and Rheumatoid …
Int. J. Mol. Sci. Untersuchungen der Trefoil factor family Peptide 1-3 in humaner Synovialfl¨¹ssigkeit und Synovialmembran und deren Bedeutung bei Arthrose und?¡­
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
APB656Hu01 Active Trefoil Factor 3 (TFF3) Cell culture; Activity Assays.
RPB656Hu01 Recombinant Trefoil Factor 3 (TFF3) Positive Control; Immunogen; SDS-PAGE; WB.
RPB656Hu02 Recombinant Trefoil Factor 3 (TFF3) Positive Control; Immunogen; SDS-PAGE; WB.
PAB656Hu02 Polyclonal Antibody to Trefoil Factor 3 (TFF3) WB; IHC; ICC; IP.
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LAB656Hu71 Biotin-Linked Polyclonal Antibody to Trefoil Factor 3 (TFF3) WB; IHC; ICC.
MAB656Hu21 Monoclonal Antibody to Trefoil Factor 3 (TFF3) WB; IHC; ICC; IP.
MAB656Hu22 Monoclonal Antibody to Trefoil Factor 3 (TFF3) WB; IHC; ICC; IP.
HEB656Hu High Sensitive ELISA Kit for Trefoil Factor 3 (TFF3) Enzyme-linked immunosorbent assay for Antigen Detection.
SEB656Hu ELISA Kit for Trefoil Factor 3 (TFF3) Enzyme-linked immunosorbent assay for Antigen Detection.
SCB656Hu CLIA Kit for Trefoil Factor 3 (TFF3) Chemiluminescent immunoassay for Antigen Detection.
LMB656Hu Multiplex Assay Kit for Trefoil Factor 3 (TFF3) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
KSB656Hu01 ELISA Kit DIY Materials for Trefoil Factor 3 (TFF3) Main materials for "Do It (ELISA Kit) Yourself".