ELISA Kit DIY Materials for Corticosteroid Binding Globulin (CBG)
SERPINA6; Transcortin; Serpin A6; Serpin Peptidase Inhibitor,Clade A,Member 6(Alpha-1 Antiproteinase; Antitrypsin)
- Product No.KSB226Ra01
- Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
- Detectable Sampleserum, plasma and other biological fluids.
- Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
- Detectable Range3.12-200ng/mL
- Applicable Sensitivity1.25ng/mL
- ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
- Downloadn/a
- UOM 96T*596T*10 96T*20 96T*50 96T*100
- FOB
US$ 1300
For more details, please contact local distributors! US$ 2166 US$ 3899 US$ 7581 US$ 12996
SPECIFITY
The Abs in the kit have high sensitivity and excellent specificity for detection of Corticosteroid Binding Globulin (CBG). No significant cross-reactivity or interference between Corticosteroid Binding Globulin (CBG) and analogues was observed.
USAGE
1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
STORAGE
Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.
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Magazine | Citations |
General Endocrinology | The Regulation of Rasd1 Expression by Glucocorticoids and Prolactin Controls Peripartum Maternal Insulin Secretion Endjournals: Source |
PLoS One | Modulation in Wistar Rats of Blood Corticosterone Compartmentation by Sex and a Cafeteria Diet PubMed: PMC3579843 |
Annals of Anatomy | Differences in colocalization of corticosteroid-binding globulin and glucocorticoid receptor immunoreactivity in the rat brain ScienceDirect: S0940960212001628 |
Physiological Reports | Adrenocortical control in the neonatal rat: ACTH‐and cAMP‐independent corticosterone production during hypoxia Pubmed: 24303136 |
Shock. | Adrenocorticotropic Hormone But Not High-Density Lipoprotein Cholesterol or Salivary Cortisol Was a Predictor of Adrenal Insufficiency in Patients With Septic Shock. Pubmed: 24667620 |
Neurobiology of Aging | Greater glucocorticoid receptor activation in hippocampus of aged rats sensitizes microglia Pubmed:25559333 |
Cell Death Differ. | Novel p53 target genes secreted by the liver are involved in non-cell-autonomous reCavia (Guinea pig )lation Pubmed:26358154 |
Journal of Exercise, Sports & Orthopedics | Impact of Season and Oral Contraceptive use on Cortisol Levelsin Physically Active Women exercise-sports-orthopedics:exercise-sports-orthop |
Impact of Season and Oral Contraceptive use on Cortisol Levels in Physically Active Women diva2:1047030 | |
Neuropharmacology | Impaired glucocorticoid-mediated HPA axis negative feedback induced by juvenile social isolation in male rats Pubmed:29407214 |
Pharmacological Reports | The impact of early-life stress on corticosteroid carrier protein levels and 11β-hydroxysteroid dehydrogenase 1 expression in adolescent rats |