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ELISA Kit DIY Materials for Protein Kinase C Beta 1 (PKCb1)

PKC-B1; PRKCB; PRKCB1

  • Product No.KSB045Rb01
  • Organism SpeciesOryctolagus cuniculus (Rabbit) Same name, Different species.
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleSerum, plasma, tissue homogenates, cell lysates and other biological fluids.
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range0.156-10ng/mL
  • Applicable Sensitivity0.061ng/mL
  • ApplicationsMain materials for "Do It(ELISA Kit) Yourself"
  • Downloadn/a
  • UOM 96T*596T*10 96T*20 96T*50 96T*100
  • FOB US$ 1241  US$ 2068  US$ 3722  US$ 7238  US$ 12408 
    For more details, please contact local distributors!

SPECIFITY

The Abs in the kit have high sensitivity and excellent specificity for detection of Protein Kinase C Beta 1 (PKCb1). No significant cross-reactivity or interference between Protein Kinase C Beta 1 (PKCb1) and analogues was observed.

USAGE

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

STORAGE

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Mol Cell Biochem. Effects of Trigonella foenum-graecum (L.) on retinal oxidative stress, and proinflammatory and angiogenic molecular biomarkers in streptozotocin-induced diabetic rats Pubmed:24242137
J Clin Diagn Res. Evaluation of Protein Kinase Cβ and PPARγ Activity in Diabetic Rats Supplemented with Momordica charantia pmc:PMC4866090
Journal of Clinical&Diagnostic Research Evaluation of Protein Kinase Cβ and PPARγ Activity in Diabetic RatsSupplemented with Momordica charantia. pubmed:27190792
Catalog No. Related products for research use of Oryctolagus cuniculus (Rabbit) Organism species Applications (RESEARCH USE ONLY!)
RPB045Rb01 Recombinant Protein Kinase C Beta 1 (PKCb1) Positive Control; Immunogen; SDS-PAGE; WB.
PAB045Rb51 Polyclonal Antibody to Protein Kinase C Beta 1 (PKCb1) WB; IHC; ICC; IP.
PAB045Rb01 Polyclonal Antibody to Protein Kinase C Beta 1 (PKCb1) WB; IHC; ICC; IP.
LAB045Rb71 Biotin-Linked Polyclonal Antibody to Protein Kinase C Beta 1 (PKCb1) WB; IHC; ICC.
KSB045Rb01 ELISA Kit DIY Materials for Protein Kinase C Beta 1 (PKCb1) Main materials for "Do It(ELISA Kit) Yourself"