ELISA Kit DIY Materials for Vitronectin (VTN)

V75; VN; VNT; Serum Spreading Factor; Somatomedin B; Complement S-Protein

  • Product No.KSB041Hu01
  • Organism SpeciesHomo sapiens (Human) Same name, Different species.
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range6.25-400ng/mL
  • Applicable Sensitivity2.74ng/mL
  • ApplicationsMain materials for "Do It(ELISA Kit) Yourself"
  • DownloadInstruction Manual
  • UOM 96T*596T*10 96T*20 96T*50 96T*100
  • FOB US$ 1197  US$ 1995  US$ 3591  US$ 6983  US$ 11970 
    For more details, please contact local distributors!

SPECIFITY

The Abs in the kit have high sensitivity and excellent specificity for detection of Vitronectin (VTN). No significant cross-reactivity or interference between Vitronectin (VTN) and analogues was observed.

USAGE

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

STORAGE

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Magazine Citations
Biomedical Engineering and Informatics Application of iTRAQ quantitative proteomics in identification of serum biomarkers in breast cancer IEEE: 6098563
PLoS ONE Identification of Altered Plasma Proteins by Proteomic Study in Valvular Heart Diseases and the Potential Clinical Significance PubMed: PMC3754973
Liver International Identification of candidate biomarkers for hepatocellular carcinoma in plasma of HCV-infected cirrhotic patients by 2-D DIGE Pubmed: 23944848
Analytical Biochemistry A multianalytical approach to investigate the effect of nanofiltration on plasma-derived factor IX clinical lots Pubmed:29154788
Journal of Materials Chemistry B Serum protein adsorption profile on BCP ceramic and influence of the elevated adsorption of adhesive proteins on the behaviour of MSCs Doi: 10.1039/C8TB02283F 
JOURNAL OF NEPHROLOGY Urinary vitronectin identifies patients with high levels of fibrosis in kidney grafts 33275196
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPB041Hu02 Recombinant Vitronectin (VTN) Positive Control; Immunogen; SDS-PAGE; WB.
RPB041Hu03 Recombinant Vitronectin (VTN) Positive Control; Immunogen; SDS-PAGE; WB.
RPB041Hu01 Recombinant Vitronectin (VTN) Positive Control; Immunogen; SDS-PAGE; WB.
PAB041Hu02 Polyclonal Antibody to Vitronectin (VTN) WB; IHC; ICC; IP.
PAB041Hu01 Polyclonal Antibody to Vitronectin (VTN) WB; IHC; ICC; IP.
PAB041Hu03 Polyclonal Antibody to Vitronectin (VTN) WB; IHC; ICC; IP.
FAB041Hu01 Anti-Vitronectin (VTN) Polyclonal Antibody Flow cytometry.
FAB041Hu03 Anti-Vitronectin (VTN) Polyclonal Antibody Flow cytometry.
LAB041Hu71 Biotin-Linked Polyclonal Antibody to Vitronectin (VTN) WB; IHC; ICC.
MAB041Hu22 Monoclonal Antibody to Vitronectin (VTN) WB; IHC; ICC; IP.
SEB041Hu ELISA Kit for Vitronectin (VTN) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB041Hu Multiplex Assay Kit for Vitronectin (VTN) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
PSB041Hu01 Antibody Pair for Vitronectin (VTN) ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays.
KSB041Hu01 ELISA Kit DIY Materials for Vitronectin (VTN) Main materials for "Do It(ELISA Kit) Yourself"