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ELISA Kit DIY Materials for Histamine (HA)

Histamin

  • Product No.KSA927Ge11
  • Organism SpeciesPan-species (General) Same name, Different species.
  • Reagent Contents Capture Antibody, Biotin-labeled Competitor, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
  • Applicable PrincipleCompetitive Inhibition ELISA for Antigen Detection
  • Detectable Range1.23-100ng/mL
  • Applicable Sensitivity0.52ng/mL
  • ApplicationsMain materials for "Do It(ELISA Kit) Yourself"
  • Downloadn/a
  • UOM 96T*596T*10 96T*20 96T*50 96T*100
  • FOB US$ 1433  US$ 2388  US$ 4298  US$ 8358  US$ 14328 
    For more details, please contact local distributors!

SPECIFITY

The Abs in the kit have high sensitivity and excellent specificity for detection of Histamine (HA). No significant cross-reactivity or interference between Histamine (HA) and analogues was observed.

USAGE

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

STORAGE

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Magazine Citations
Allergy & Clinical Immunology PEST-domain-enriched tyrosine phosphatase and glucocorticoids as regulators of anaphylaxis in mice Pubmed: 21981059
Asian Pacific Journal of Allergy and Immunology Comparison of sensitization between β-lactoglobulin and its hydrolysates Digital: 875
J Chromatogr A Integrative analysis of proteomics and metabolomics of anaphylactoid reaction induced by Xuesaitong injection PubMed: 26372445
Neurogastroenterology & Motility Expression of inducible nitric oxide synthase in mast cells contributes to the reCavia (Guinea pig )lation of inflammatory cytokines in irritable bowel syndrome with diarrhea Pubmed:26940641
Italian Journal of Animal Science Induction of immune responses and allergic reactions in piglets by injecting glycinin doi:1828051X.2016.1144488
Int J Clin Exp Pathol Effects of Dectin-1 on the mast cells in allergic conjunctivitis and its underlying mechanism files:ijcep0019305.pdf
PLoS One. Proteomics Study on Nonallergic Hypersensitivity Induced by Compound 4880 and Ovalbumin pmc:PMC4734762
Int Immunopharmacol. Evaluation of anaphylactoid constituents in vitro and in vivo. pubmed:27984711
Journal of Cosmet Dermatol. Ultraviolet A photosensitivity profile of dexchlorpheniramine maleate and promethazine-based creams: Anti-inflammatory,antihistaminic, and skin barrier protection properties DOI: 10.1111/jocd.12349
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES  Adipose Tissue-Derived Mesenchymal Stem Cell Modulates the Immune Response of Allergic Rhinitis in a Rat Model Pubmed: 30781605
Royal Society Open Science Metabolomics analysis of baicalin on ovalbumin-sensitized allergic rhinitis rats
International Journal of Pharmaceutical Sciences and Research MOTION SICKNESS INDUCES PHYSIOLOGICAL AND NEURONAL ALTERATIONS IN MOUSE MODEL
Allergy Artemisia annua sublingual immunotherapy for seasonal allergic rhinitis: a randomized controlled trial Pubmed: 32030780
MOLECULAR IMMUNOLOGY Animal models for analysis of hypersensitivity reactions to Shuanghuanglian injection Pubmed: 32302806
bioscience reports Protective effect of Asarum sieboldii essential oil on ovalbumin induced allergic rhinitis in rat Pubmed: 32395767
Nature Communications A vaccine-based nanosystem for initiating innate immunity and improving tumor immunotherapy Pubmed: 32332752
CCR3‑shRNA promotes apoptosis and inhibits chemotaxis and degranulation of mouse mast cells Pubmed: 32742345
ANALYTICA CHIMICA ACTA Multiplexed fluorescence immunoassay combined with magnetic separation using upconversion nanoparticles as multicolor labels for the simultaneous detection of … Pubmed: 32892931
Annals of Tropical Medicine & Public Health Study of some hematological and immunological parameters associated with the infection of intestinal parasites in the holy city of Kerbala, Iraq
pharmacol res& perspect Pharmacokinetics and safety of TCMCB07, a melanocortin-4 antagonist peptide in dogs 34014033
Catalog No. Related products for research use of Pan-species (General) Organism species Applications (RESEARCH USE ONLY!)
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PAA927Ge01 Polyclonal Antibody to Histamine (HA) ELISA, CLIA. / IHC-Fr, ICC, IP (predicted).
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MAA927Ge21 Monoclonal Antibody to Histamine (HA) ELISA, CLIA. / IHC-Fr, ICC, IP (predicted).
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LMA927Ge Multiplex Assay Kit for Histamine (HA) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
KSA927Ge11 ELISA Kit DIY Materials for Histamine (HA) Main materials for "Do It(ELISA Kit) Yourself"