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ELISA Kit DIY Materials for Bradykinin (BK)

  • Product No.KSA874Hu11
  • Organism SpeciesHomo sapiens (Human) Same name, Different species.
  • Reagent Contents Capture Antibody, Biotin-labeled Competitor, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
  • Applicable PrincipleCompetitive Inhibition ELISA for Antigen Detection
  • Detectable Range6.17-500pg/mL
  • Applicable Sensitivity2.62pg/mL
  • ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
  • Downloadn/a
  • UOM 96T*596T*10 96T*20 96T*50 96T*100
  • FOB US$ 1134  US$ 1890  US$ 3402  US$ 6615  US$ 11340 
    For more details, please contact local distributors!

SPECIFITY

The Abs in the kit have high sensitivity and excellent specificity for detection of Bradykinin (BK). No significant cross-reactivity or interference between Bradykinin (BK) and analogues was observed.

USAGE

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

STORAGE

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Magazine Citations
Journal of Cardiology and Clinical Research Bradykinin-Induced Shock Increase Exhaled Nitric Oxide, Complement Activation and Cytokine Production in Pigs handle:10037
Journal of Allergy and Clinical Immunology A transcriptomic study of Hereditary Angioedema attacks Pubmed:29729940
Toxicology Bradykinin contributes to immune liver injury via B2R receptor-mediated pathways in trichloroethylene sensitized mice: a role in Kupffer cell activation Pubmed: 30685357
Thoracic Cancer Increased expression of TRPV1 in patients with acute or chronic cough after lung cancer surgery Pubmed: 30883022
Respiratory Physiology & Neurobiology Effects of nanoparticles on Neuroinflammation in a Mouse Model of Asthma Pubmed: 31542455
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
SPA874Hu01 Synthetic Bradykinin (BK) Immunogen; Blocking Peptide.
CPA874Hu31 KLH Conjugated Bradykinin (BK) Immunogen; SDS-PAGE; WB.
CPA874Hu11 BSA Conjugated Bradykinin (BK) Immunogen; SDS-PAGE; WB.
CPA874Hu21 OVA Conjugated Bradykinin (BK) Immunogen; SDS-PAGE; WB.
PAA874Hu01 Polyclonal Antibody to Bradykinin (BK) WB; IHC; ICC; IP.
MAA874Hu21 Monoclonal Antibody to Bradykinin (BK) WB; IHC; ICC; IP.
MAA874Hu24 Monoclonal Antibody to Bradykinin (BK) WB; IHC; ICC; IP.
CEA874Hu ELISA Kit for Bradykinin (BK) Enzyme-linked immunosorbent assay for Antigen Detection.
HEA874Hu High Sensitive ELISA Kit for Bradykinin (BK) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA874Hu Multiplex Assay Kit for Bradykinin (BK) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
KSA874Hu11 ELISA Kit DIY Materials for Bradykinin (BK) Main materials for "Do It (ELISA Kit) Yourself".