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ELISA Kit DIY Materials for Dopamine (DA)

2-(3,4-Dihydroxyphenyl)ethylamine; 3,4-Dihydroxyphenethylamine; 3-Hydroxytyramine; Intropin; Revivan; Oxytyramine; Dopamine Hydrochloride

SPECIFITY

The Abs in the kit have high sensitivity and excellent specificity for detection of Dopamine (DA). No significant cross-reactivity or interference between Dopamine (DA) and analogues was observed.

USAGE

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

STORAGE

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Magazine Citations
Renal Failure Circulating Levels of Renalase, Norepinephrine, and Dopamine in Dialysis Patients Pubmed: 23530612
Molecular Neurobiology Ursodeoxycholic Acid Ameliorates Apoptotic Cascade in the Rotenone Model of Parkinson’s Disease: Modulation of Mitochondrial Perturbations Pubmed:25502462
Molecular Neurobiology Bone Marrow-Derived Endothelial Progenitor Cells Protect Against Scopolamine-Induced Alzheimer-Like Pathological Aberrations Pubmed:25526861
Cardiology. Effect of salt intake and potassium supplementation on urinary renalase and serum dopamine levels in Chinese adults Pubmed:25824645
Eur J Pharmacol Nalbuphine could decrease the rewarding effect induced by tramadol in mice while enhancing its antinociceptive activity PubMed: 25843409
Neuropsychiatr Dis Treat Catha edulis chewing effects on treatment of paranoid schizophrenic patients PubMed: 25926735
Journal of Neurochemistry Neuroprotective effects of vildagliptin in rat rotenone Parkinson's disease model: role of RAGE‐NFκB and Nrf2‐antioxidant signaling pathways PubMed: 25752913
Beni-Suef University Journal of Basic and Applied Sciences Modulatory effect of cilostazol on tramadol-induced behavioral and neurochemical alterations in rats challenged across the forced swim despair test science:S2314853516300166
Neuroscience Imipramine and amitriptyline ameliorate the rotenone model of Parkinson's disease in rats pubmed:27365173
Metab Brain Dis. Neuroprotective effect of Cucumis melo Var. flexuosus leaf extract on the brains of rats with streptozotocin-induced diabetes. pubmed:27488111
Nutrition Ginkgo biloba extract alleviates oxidative stress and some neurotransmitters changes induced by aluminum chloride in rats S0899900716302350
Nutrition Ginkgo Biloba Extract Alleviates Oxidative Stress and Some Neurotransmitters Changes Induced by Aluminum Chloride in Rats pubmed:28241996
Effects of Junk Foods on Brain Neurotransmitters (Dopamine and Serotonin) and some Biochemical Parameters in Albino Rats article_1486_c0fa9f8afe5f70b407e7195bcfd55c70
Behavioural Brain Research High Fat Diet feeding induced insulin resistance exacerbates 6-OHDA mediated neurotoxicity and behavioral abnormalities in rats Pubmed:29842916
Toxicology Reports Neuro-and nephroprotective effect of grape seed proanthocyanidin extract against carboplatin and thalidomide through modulation of inflammation, tumor … Pubmed:29854627
Molecular and Cellular Biochemistry Repetitive transcranial magnetic stimulation inhibits Sirt1/MAO-A signaling in the prefrontal cortex in a rat model of depression and cortex-derived astrocytes Pubmed:28948423
Physiology & Behavior  Linagliptin potentiates the effect of l-dopa on the behavioural, biochemical and immunohistochemical changes in experimentally-induced Parkinsonism: Role of toll … Pubmed:29410018
Behavioural Brain Research Possible mechanisms of prenatal cold stress induced-anxiety-like behavior depression in offspring rats Pubmed: 30423388
Indian Journal of Ophthalmology Dopamine levels in human tear fluid Pubmed: 30574889
Journal of Taibah University for Science Neurotoxicity and inflammation induced by individual and combined exposure to iron oxide nanoparticles and silver nanoparticles
Metabolic Brain Disease Soya bean rich diet is associated with adult male rat aggressive behavior: relation to RF amide-related peptide 3-aromatase-neuroestrogen pathway in the brain Pubmed: 31134480
Functional activity of p-glycoprotein in blood-brain barrier during experimental par-kinson's syndrome
Neurotoxicity of Mn3O4 nanoparticles: Apoptosis and dopaminergic neurons damage pathway Pubmed: 31740235
Neurochemistry International Polydatin protects SH-SY5Y in models of Parkinson's disease by promoting Atg5-mediated but parkin-independent autophagy Pubmed: 31926197
Neurochemistry International Iron overload induced by IRP2 gene knockout aggravates symptoms of Parkinson's disease Pubmed: 31904393
Journal of neural transmission (Vienna, Austria : 1996) Neurotransmitter system aberrations in patients with drug addiction Pubmed: 32804296
Acupunct Med Electroacupuncture alleviates intestinal inflammation and barrier dysfunction by activating dopamine in a rat model of intestinal ischaemia Pubmed: 32517478
Effect of metformin and indole-3-carbinol on a rat model of Parkinson's disease induced by 6-hydroxydopamine
South African Journal of Botany Neuroprotective effects of pulicaria undulata essential oil in rotenone model of parkinson's disease in rats: Insights into its anti-inflammatory and anti-oxidant effects
ACTA PHARMACOLOGICA SINICA The vascular endothelial growth factor trap aflibercept induces vascular dysfunction and hypertension via attenuation of eNOS/NO signaling in mice 33303990
Neural Regeneration Research Glucagon-like peptide-1/glucose-dependent insulinotropic polypeptide dual receptor agonist DA-CH5 is superior to exendin-4 in protecting neurons in the 6?¡­ 33433498
International Journal of?Nanomedicine Water-Soluble Carbon Dots in Cigarette Mainstream Smoke: Their Properties and the Behavioural, Neuroendocrinological, and Neurotransmitter Changes They ¡­ 33762821
CRYOBIOLOGY O-GlcNAc/Akt pathway regulates glucose metabolism and reduces apoptosis in liver of piglets with acute cold stress 33651993
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA A pair of dopamine neurons mediate chronic stress signals to induce learning deficit in Drosophila melanogaster 34654742
ACS Chem Neurosci Neuroprotective Effects of Trehalose and Sodium Butyrate on Preformed Fibrillar Form of α-Synuclein-Induced Rat Model of Parkinson's Disease 34197084
Exp Brain Res Effects of early life stress during stress hyporesponsive period (SHRP) on anxiety and curiosity in adolescent rats Pubmed:35141770
Catalog No. Related products for research use of Pan-species (General) Organism species Applications (RESEARCH USE ONLY!)
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