ELISA Kit DIY Materials for Prolactin (PRL)
LTH; Luteotropic Hormone
- Product No.KSA846Bo01
- Organism SpeciesBos taurus; Bovine (Cattle) Same name, Different species.
- Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
- Detectable SampleSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
- Detectable Range1.56-100ng/mL
- Applicable Sensitivity0.64ng/mL
- ApplicationsMain materials for "Do It(ELISA Kit) Yourself"
- Downloadn/a
- UOM 96T*596T*10 96T*20 96T*50 96T*100
- FOB
US$ 1512
For more details, please contact local distributors! US$ 2520 US$ 4536 US$ 8820 US$ 15120
SPECIFITY
The Abs in the kit have high sensitivity and excellent specificity for detection of Prolactin (PRL). No significant cross-reactivity or interference between Prolactin (PRL) and analogues was observed.
USAGE
1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
STORAGE
Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.
Support Pack
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Magazine | Citations |
The Journal of Biological Chemistry | In Vivo Evidence for Epidermal Growth Factor Receptor (EGFR)-mediated Release of Prolactin from the Pituitary Gland Jcb: 39297 |
Journal of Clinical Neuroscience | TLR9 expression is associated with prognosis in patients with glioblastoma multiforme ScienceDirect: S0967586811003572 |
Sensors and Actuators B: Chemical | Determination of prolactin hormone in serum and urine using an electrochemical immunosensor based on poly(pyrrolepropionic acid)/carbon nanotubes hybrid modified electrodes Sciencedirect:S0925400514000720 |
Veterinary Word | Effect of extended photoperiod during winter on growth and onset of puberty in Murrah buffalo heifers Pubmed:27051212 |
Journal of Dairy Science | Effect of thermal stress on physiological, hormonal and haematological parameters in Tharparkar and Karan Fries calves publication:306322503 |
Stem Cell Research & Therapy | Differentiation of human umbilical cord Wharton’s jelly-derived mesenchymal stem cells into endometrial cells pubmed:29096715 |
Stem Cell Research & Therapy | The role of mesenchymal stem cells in chemotherapy-induced gonadotoxicity Pubmed:30021657 |
Frontiers in Molecular Neuroscience | Impact of Triclosan on Female Reproduction through Reducing Thyroid Hormones to Suppress Hypothalamic Kisspeptin Neurons in Mice Pubmed:29403355 |
Toxicology and Applied Pharmacology | The antipsychotics sulpiride induces fatty liver in rats via phosphorylation of insulin receptor substrate-1 at Serine 307-mediated adipose tissue insulin resistance Pubmed:29551354 |
Journal of Cellular Biochemistry | The prolactin‐release inhibitor paeoniflorin suppresses proliferation and induces apoptosis in prolactinoma cells via the mitochondria‐dependent pathway Pubmed:29388711 |
Journal of Cellular and Molecular Medicine | Metformin inhibits growth and prolactin secretion of pituitary prolactinoma cells and xenografts Pubmed: 30334324 |
BMC Veterinary Research | Hormonal and metabolic indicators before and after farrowing in sows affected with postpartum dysgalactia syndrome Pubmed: 30404636 |
International Journal of Basic and Clinical Endocrinology | Expression of prolactin receptors in the duodenum, kidneys and skeletal system during physiological and sulpiride-induced hyperprolactinaemia Pubmed: 30143940 |
MORPHOLOGY AND PATHOMORPHOLOGY | Morphological and Biochemical Characteristics of Prostate Hyperplasia during Sulpiride Treatment Pubmed: 32152847 |
JOURNAL OF DAIRY SCIENCE | Effect of heat stress during the early and late dry period on mammary gland development of Holstein dairy cattle Pubmed: 32684470 |
BioMed Research International | Pathophysiological Changes in Female Rats with Estrous Cycle Disorder Induced by Long-Term Heat Stress Pubmed: 32685488 |
Vet Microbiol | Prolactin affects the disappearance of ALV-J viremia in vivo and inhibits viral infection 34391195 |
Antioxidants (Basel) | Effects of a Nanoencapsulated Moringa Leaf Ethanolic Extract on the Physiology, Metabolism and Reproductive Performance of Rabbit Does during Summer 34439574 |
Nutrients | Yogurt Enriched with Inulin Ameliorated Reproductive Functions and Regulated Gut Microbiota in Dehydroepiandrosterone-Induced Polycystic Ovary Syndrome Mice Pubmed:35057459 |
Catalog No. | Related products for research use of Bos taurus; Bovine (Cattle) Organism species | Applications (RESEARCH USE ONLY!) |
APA846Bo01 | Active Prolactin (PRL) | Cell culture; Activity Assays. |
RPA846Bo01 | Recombinant Prolactin (PRL) | Positive Control; Immunogen; SDS-PAGE; WB. |
PAA846Bo01 | Polyclonal Antibody to Prolactin (PRL) | WB; IHC; ICC; IP. |
MAA846Bo21 | Monoclonal Antibody to Prolactin (PRL) | WB; IHC; ICC; IP. |
SEA846Bo | ELISA Kit for Prolactin (PRL) | Enzyme-linked immunosorbent assay for Antigen Detection. |
CEA846Bo | ELISA Kit for Prolactin (PRL) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMA846Bo | Multiplex Assay Kit for Prolactin (PRL) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |
KSA846Bo01 | ELISA Kit DIY Materials for Prolactin (PRL) | Main materials for "Do It(ELISA Kit) Yourself" |