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ELISA Kit DIY Materials for Adrenocorticotropic Hormone (ACTH)
Corticotropin; Adrenocorticotropin
- Product No.KSA836Ra11
- Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- Reagent Contents Capture Antibody, Biotin-labeled Competitor, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
- Detectable SampleSerum, plasma and other biological fluids.
- Applicable PrincipleCompetitive Inhibition ELISA for Antigen Detection
- Detectable Range12.35-1000pg/mL
- Applicable Sensitivity5.25pg/mL
- ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
- Downloadn/a
- UOM 96T*596T*10 96T*20 96T*50 96T*100
- FOB
US$ 1094
US$ 1824
US$ 3283
US$ 6384
US$ 10944
For more details, please contact local distributors!
SPECIFITY
The Abs in the kit have high sensitivity and excellent specificity for detection of Adrenocorticotropic Hormone (ACTH). No significant cross-reactivity or interference between Adrenocorticotropic Hormone (ACTH) and analogues was observed.
USAGE
1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
STORAGE
Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.
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| Magazine | Citations |
| Respir Res. | Intracerebroventricular injection of leukotriene B4 attenuates antigen-induced asthmatic response via BLT1 receptor stimulating HPA-axis in sensitized rats. PubMed: 20403205 |
| Toxicology Letters | Regulation of corticosterone secretion is modified by PFOS exposure at different levels of the hypothalamic–pituitary–adrenal axis in adult male rats Pubmed:24440345 |
| Mol Med Rep. | Ursolic acid suppresses growth and adrenocorticotrophic hormone secretion in AtT20 cells as a potential agent targeting adrenocorticotrophic hormone-producing pituitary adenoma. Pubmed:24682498 |
| Peptides | Differential stress response in rats subjected to chronic mild stress is accompanied by changes in CRH-family gene expression at the pituitary level Pubmed:25236411 |
| Journal of Animal and Feed Sciences | Interdependence of the peripheral metabolism of glucocorticoids and thyroid hormones under calorie deficit in rats at different ages Researchgate:Source |
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| Cell Tissue Res | CXCL10/CXCR3 signaling mediates inhibitory action by interferon-gamma on CRF-stimulated adrenocorticotropic hormone (ACTH) release PubMed: 26572542 |
| Neuroscience Letters | Electroacupuncture reCavia (Guinea pig )late hypothalamic–pituitary–adrenal axis and enhance hippocampal serotonin system in a rat model of depression Pubmed:26773866 |
| Neural Regeneration Research | Moxibustion upreCavia (Guinea pig )lates hippocampal progranulin expression. Pubmed:27212922 |
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| brain research bulletin | Activation of ATP-sensitive potassium channel by iptakalim normalizes stress-induced HPA axis disorder and depressive behaviour by alleviating inflammation and oxidative stress in mouse hypothalamus. pubmed:28161195 |
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| Hormones and Behavior | Acth-induced model of depression resistant to tricyclic antidepressants: Neuroendocrine and behavioral changes and influence of long-term magnesium … Pubmed:30025718 |
| Frontiers in Pharmacology | Vaccinium bracteatum Leaf Extract Reverses Chronic Restraint Stress-Induced Depression-Like Behavior in Mice: Regulation of Hypothalamic-Pituitary … Pubmed:30038568 |
| Molecular and Cellular Endocrinology | Metformin suppresses growth and adrenocorticotrophic hormone secretion in mouse pituitary corticotroph tumor AtT20 cells 10.1016:j.mce.2018.07.007 |
| Frontiers in Behavioral Neuroscience | Chronic hippocampal abnormalities and blunted HPA axis in an animal model of repeated unpredictable stress Pubmed:30079015 |
| Placenta | Human placentophagy: Effects of dehydration and steaming on hormones, metals and bacteria in placental tissue Pubmed:29941176 |
| BMC Veterinary Research | Hypothalamus-pituitary-adrenal axis involves in anti-viral ability through regulation of immune response in piglets infected by highly pathogenic porcine … Pubmed:29540178 |
| European Journal of Applied Physiology | Comparison between men and women of volume regulating hormones and aquaporin-2 excretion following graded central hypovolemia Pubmed: 30564880 |
| Cyber Leninka | ТЭС-ТЕРАПИЯ КАК МЕТОД ПРЕДУПРЕЖДЕНИЯ ДЕЗАДАПТАЦИИ У САМЦОВ КРЫС С ВЫСОКОЙ СТРЕССОУСТОЙЧИВОСТЬЮ |
| Serbian Journal of Experimental and Clinical Research | Assessment of the TDCS Influence on Stress-Induced Disorders in Rats with Low Stress Sustainability and Endurance |
| Scientific Reports | Mice lacking Casp1, Ifngr and Nos2 genes exhibit altered depressive-and anxiety-like behaviour, and gut microbiome composition Pubmed: 31015500 |
| alcoholism-clinical and experimental research | Persistent changes in stress‐regulatory genes in pregnant woman or a child with prenatal alcohol exposure Pubmed: 31329297 |
| Hormones and Behavior | Hypothalamus-pituitary-adrenal axis imbalance and inflammation contribute to sex differences in separation-and restraint-induced depression Pubmed: 32165183 |
| Prog Neuropsychopharmacol Biol Psychiatry | Progress in Neuropsychopharmacology & Biological Psychiatry Pubmed: 17134810 |
| Evid Based Complement Alternat Med | So-Ochim-Tang-Gamibang, a Traditional Herbal Formula, Ameliorates Depression by Regulating Hyperactive Glucocorticoid Signaling In Vitro and In Vivo Pubmed: 33224257 |
| PROGRESS IN NEURO-PSYCHOPHARMACOLOGY & BIOLOGICAL PSYCHIATRY | Repeated unpredictable stress and social isolation induce chronic HPA axis dysfunction and persistent abnormal fear memory Pubmed: 32682873 |
| Aging Cell | Altered glucocorticoid metabolism represents a feature of macroph‐aging Pubmed: 32463582 |
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| Catalog No. | Related products for research use of Rattus norvegicus (Rat) Organism species | Applications (RESEARCH USE ONLY!) |
| CEA836Ra | ELISA Kit for Adrenocorticotropic Hormone (ACTH) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| CCA836Ra | CLIA Kit for Adrenocorticotropic Hormone (ACTH) | Chemiluminescent immunoassay for Antigen Detection. |
| LMA836Ra | Multiplex Assay Kit for Adrenocorticotropic Hormone (ACTH) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |
| KSA836Ra11 | ELISA Kit DIY Materials for Adrenocorticotropic Hormone (ACTH) | Main materials for "Do It (ELISA Kit) Yourself". |