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ELISA Kit DIY Materials for Amylin

IAPP; IAP; DAP; Islet Amyloid Polypeptide; Insulinoma Amyloid Polypeptide; Diabetes-associated peptide

  • Product No.KSA812Ra11
  • Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
  • Reagent Contents Capture Antibody, Biotin-labeled Competitor, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
  • Applicable PrincipleCompetitive Inhibition ELISA for Antigen Detection
  • Detectable Range6.17-500pg/mL
  • Applicable Sensitivity2.83pg/mL
  • ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
  • Downloadn/a
  • UOM 96T*596T*10 96T*20 96T*50 96T*100
  • FOB US$ 1231  US$ 2052  US$ 3694  US$ 7182  US$ 12312 
    For more details, please contact local distributors!

SPECIFITY

The Abs in the kit have high sensitivity and excellent specificity for detection of Islet Amyloid Polypeptide (IAPP). No significant cross-reactivity or interference between Islet Amyloid Polypeptide (IAPP) and analogues was observed.

USAGE

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

STORAGE

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Magazine Citations
plos ONE Differential Effect of Amylin on Endothelial-Dependent Vasodilation in Mesenteric Arteries from Control and Insulin Resistant Rats PubMed: 25807378
Nutrients Glucagon Shows Higher Sensitivity than Insulin to Grapeseed Proanthocyanidin Extract (GSPE) Treatment in Cafeteria-Fed Rats 33810265
Catalog No. Related products for research use of Rattus norvegicus (Rat) Organism species Applications (RESEARCH USE ONLY!)
RPA812Ra01 Recombinant Amylin Positive Control; Immunogen; SDS-PAGE; WB.
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PAA812Ra01 Polyclonal Antibody to Amylin WB; IHC; ICC; IP.
PAA812Ra08 Polyclonal Antibody to Amylin WB; IHC; ICC; IP.
MAA812Ra21 Monoclonal Antibody to Amylin WB; IHC; ICC; IP.
CEA812Ra ELISA Kit for Amylin Enzyme-linked immunosorbent assay for Antigen Detection.
MEA812Ra Mini Samples ELISA Kit for Amylin Enzyme-linked immunosorbent assay for Antigen Detection.
LMA812Ra Multiplex Assay Kit for Amylin ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
KSA812Ra11 ELISA Kit DIY Materials for Amylin Main materials for "Do It (ELISA Kit) Yourself".