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ELISA Kit DIY Materials for Mucin 5 Subtype AC (MUC5AC)
TBM; MUC5; LeB; Oligomeric Mucus/Gel-Forming; Mucin 5,Subtypes A And C,Tracheobronchial/Gastric; Gastric mucin; Lewis B blood group antigen; Major airway glycoprotein
- Product No.KSA756Mu01
- Organism SpeciesMus musculus (Mouse) Same name, Different species.
- Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
- Detectable SampleSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
- Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
- Detectable Range0.156-10ng/mL
- Applicable Sensitivity0.064ng/mL
- ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
- Downloadn/a
- UOM 96T*596T*10 96T*20 96T*50 96T*100
- FOB
US$ 1166
US$ 1944
US$ 3499
US$ 6804
US$ 11664
For more details, please contact local distributors!
SPECIFITY
The Abs in the kit have high sensitivity and excellent specificity for detection of Mucin 5 Subtype AC (MUC5AC). No significant cross-reactivity or interference between Mucin 5 Subtype AC (MUC5AC) and analogues was observed.
USAGE
1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
STORAGE
Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.
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| Magazine | Citations |
| Annals of surgical oncology | Pancreatic cyst fluid and serum mucin levels predict dysplasia in intraductal papillary mucinous neoplasms of the pancreas. PubMed: 20717734 |
| Investigative Ophthalmology & Visual Science | Entrapment of Conjunctival Goblet Cells by Desiccation-Induced Cornification PubMed: 21421863 |
| Cell & Molecular Biology | Role of aquaporin 5 in antigen-induced airway inflammation and mucous hyperproduction in mice PubMed: 20550619 |
| Mucosal Immunology | Homeostatic control of conjunctival mucosal goblet cells by NKT-derived IL-13 Nature: 201082a |
| European Journal of Pharmacology | Naringin attenuates EGF-induced MUC5AC secretion in A549 cells by suppressing the cooperative activities of MAPKs-AP-1 and IKKs-IκB-NF-κB signaling pathways ScienceDirect: S0014299912005523 |
| PLoS One | Baicalein Reduces Airway Injury in Allergen and IL-13 Induced Airway Inflammation PubMed: PMC3639905 |
| Respiratory Researh | Pyocyanin-induced mucin production is associated with redox modification of FOXA2 PubMed: PMC3765780 |
| Surgery | A novel serum marker for biliary tract cancer: Diagnostic and prognostic values of quantitative evaluation of serum mucin 5AC (MUC5AC) Pubmed: 24468034 |
| Biochemical and Biophysical Research Communications | The human cathelicidin LL-37 enhances airway mucus production in chronic obstructive pulmonary disease Pubmed:24291709 |
| Journal of Proteomics | Proteomic analysis of cancer and mesothelial cells reveals an increase in Mucin 5AC during ovarian cancer and peritoneal interaction Pubmed:24726482 |
| Experimental Lung Research | The human Cathelicidin LL-37 induces MUC5AC mucin production by airway epithelial cells via TACE-TGF-α-EGFR pathway Pubmed:24901072 |
| Surgery.? | Assessment of bile and serum mucin5AC in cholangiocarcinoma: Diagnostic performance and biologic significance Pubmed:25151557 |
| Allergology International | Leukotriene E 4 induces MUC5AC release from human airway epithelial NCI-H292 cells Pubmed:25838093 |
| Respirology | Secreted mucins and airway bacterial colonization in non-CF bronchiectasis PubMed: 26172851 |
| S | Isabela Casagrande Jeremias Teses: Disponiveis |
| American Thoracic Society International Conference Abstracts | Mucin Secretion In Asthmatics With Osmotic Airway Hyperresponsiveness doi:10.1164 |
| Deletion Polymorphism and Chronic Obstructive Pulmonary Disease | ReCavia (Guinea pig )lation of Viral Infection-induced Airway Remodeling Cytokine Production by the TLR3-EGFR Signaling Pathway in Human Bronchial Epithelial Cells Pubmed:27075970 |
| Int J Clin Exp Med | The influence of long-term corneal contact lens wearing on the stability and quality of tear film in Qinghai files:ijcem0020163.pdf |
| Ann Am Thorac Soc | Airway Mucin 2 Is Decreased in Patients with Severe Chronic Obstructive Pulmonary Disease with Bacterial Colonization doi:10.1513 |
| Sci Rep | Impact of Cigarette Smoking on Tear Function and Correlation between Conjunctival Goblet Cells and Tear MUC5AC Concentration in Office Workers Pubmed:27297822 |
| Cytokine. | Interleukin-33 induces mucin gene expression and goblet cell hyperplasia in human nasalepithelial cells. pubmed:27776277 |
| J Virol. | Mutating the CX3C Motif in the G Protein Should Make a Live Respiratory Syncytial Virus Vaccine Safer and More Effective. pubmed:28275196 |
| Respiratory Physiology & Neurobiology | Peroxiredoxin 6 suppresses Muc5ac overproduction in LPS-induced airway inflammation through H2O2-EGFR-MAPK signaling pathway. pubmed:27884794 |
| Journal of Immunology Research | Beneficial effects of prebiotic Saccharomyces cerevisiae mannan on allergic asthma mouse models pubmed:28835901 |
| BASIC INVESTIGATION | Efficacy of Sodium Hyaluronate in Murine Diabetic Ocular Surface Diseases pubmed:28644234 |
| International Immunopharmacology | Clarithromycin inhibits TNF-α-induced MUC5AC mucin gene expression via the MKP-1-p38MAPK-dependent pathway pubmed:28550735 |
| Секреторно-экссудативная реакция слизистой носа на воздействие холодного воздуха у больных бронхиальной астмой 10.20538/1682-0363-2017-2-146-158 | |
| Cellular Microbiology | An adherent mucus layer attenuates the genotoxic effect of colibactin. pubmed:29156489 |
| Respiratory Research | IL-4-induced caveolin-1-containing lipid rafts aggregation contributes to MUC5AC synthesis in bronchial epithelial cells pubmed:28931396 |
| Chemotherapy | Oxytetracycline Inhibits Mucus Secretion and Inflammation in Human Airway Epithelial Cells. pubmed:28578323 |
| Allergy | Th2 cytokines orchestrate the secretion of MUC 5 AC and MUC 5B in IL‐5 positive chronic rhinosinusitis with nasal polyps Pubmed:29802623 |
| European Journal of Pharmacology | Salvianolic acid B improves airway hyperresponsiveness by inhibiting MUC5AC overproduction associated with Erk1/2/P38 signaling Pubmed:29382534 |
| Inflammation | Plantamajoside Inhibits Lipopolysaccharide-Induced MUC5AC Expression and Inflammation through Suppressing the PI3K/Akt and NF-κB Signaling Pathways in … Pubmed:29349683 |
| Pulmonary Pharmacology and Therapeutics | In vivo and in vitro investigation of anti-inflammatory and mucus-regulatory activities of a fixed combination of thyme and primula extracts Pubmed:29698781 |
| European Respiratory Society | Profiling of healthy and asthmatic airway smooth muscle cells following IL-1β treatment: a novel role for CCL20 in chronic mucus hyper-secretion Pubmed:29946002 |
| Clinical and Experimental Ophthalmology | Development of a highly sensitive and reliable enzyme-linked immunosorbent assay for MUC5aC in human tears extracted from schirmer strips Pubmed: 30214141 |
| international journal of biological macromolecules | Increased expression of long non-coding RNA SNHG16 correlates with tumor progression and poor prognosis in non-small cell lung cancer Pubmed: 30287374 |
| Journal of Investigative Medicine | The characteristics and clinical significance of mucin levels in bronchoalveolar lavage fluid of patients with interstitial lung disease Pubmed: 30573494 |
| Phytomedicine | Regulation effects of naringin on diesel particulate matter-induced abnormal airway surface liquid secretion Pubmed: 31301536 |
| Scientific Reports | Regional comparison of Goblet cell number and Area in exposed and covered Dry eyes and their Correlation with Tear MUC5AC Pubmed: 32076085 |
| Eur J Pharmacol | TLR3 inhibitor and tyrosine kinase inhibitor attenuate cigarette smoke/poly I: C-induced airway inflammation and remodeling by the EGFR/TLR3/MAPK … Pubmed: 33068589 |
| journal of ethnopharmacology | Effective-component compatibility of Bufei Yishen formula II inhibits mucus hypersecretion of chronic obstructive pulmonary disease rats by regulating EGFR/PI3K … Pubmed: 32344236 |
| Less Airway Inflammation and Goblet Cell Metaplasia in an IL-33-Induced Asthma Model of Leptin-Deficient Obese Mice | |
| Exp Mol Pathol | The regulatory role of SFRP5/WNT5A axis in allergic rhinitis through inhibiting JNK pathway activation and lowering mucin generation in human nasal?¡ 33285209 |
| FEBS Open Bio | Porphyromonas gingivalis gingipains potentially affect MUC5AC gene expression and protein levels in respiratory epithelial cells 33332733 |
| Cell Rep | Generation of functional conjunctival epithelium, including goblet cells, from human iPSCs 33535050 |
| Catalog No. | Related products for research use of Mus musculus (Mouse) Organism species | Applications (RESEARCH USE ONLY!) |
| RPA756Mu02 | Recombinant Mucin 5 Subtype AC (MUC5AC) | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA756Mu01 | Recombinant Mucin 5 Subtype AC (MUC5AC) | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA756Mu03 | Recombinant Mucin 5 Subtype AC (MUC5AC) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA756Mu01 | Polyclonal Antibody to Mucin 5 Subtype AC (MUC5AC) | WB; IHC; ICC; IP. |
| PAA756Mu02 | Polyclonal Antibody to Mucin 5 Subtype AC (MUC5AC) | WB; IHC; ICC; IP. |
| PAA756Mu03 | Polyclonal Antibody to Mucin 5 Subtype AC (MUC5AC) | WB; IHC; ICC; IP. |
| LAA756Mu81 | FITC-Linked Polyclonal Antibody to Mucin 5 Subtype AC (MUC5AC) | WB; IHC; ICC; IF. |
| LAA756Mu71 | Biotin-Linked Polyclonal Antibody to Mucin 5 Subtype AC (MUC5AC) | WB; IHC; ICC. |
| IEA756Mu | Instant ELISA Kit for Mucin 5 Subtype AC (MUC5AC) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SEA756Mu | ELISA Kit for Mucin 5 Subtype AC (MUC5AC) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCA756Mu | CLIA Kit for Mucin 5 Subtype AC (MUC5AC) | Chemiluminescent immunoassay for Antigen Detection. |
| LMA756Mu | Multiplex Assay Kit for Mucin 5 Subtype AC (MUC5AC) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |
| KSA756Mu01 | ELISA Kit DIY Materials for Mucin 5 Subtype AC (MUC5AC) | Main materials for "Do It (ELISA Kit) Yourself". |