≡

ELISA Kit DIY Materials for Prothrombin Fragment 1+2 (F1+2)

SPECIFITY

The Abs in the kit have high sensitivity and excellent specificity for detection of Prothrombin Fragment 1+2 (F1+2). No significant cross-reactivity or interference between Prothrombin Fragment 1+2 (F1+2) and analogues was observed.

USAGE

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

STORAGE

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

Support Pack

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
Critical Care Disseminated intravascular coagulation or acute coagulopathy of trauma shock early after trauma? An observational study BioMed: cc10553
Journal of Vascular Surgery Changes in thrombin generation, fibrinolysis, platelet and endothelial cell activity, and inflammation following endovascular abdominal aortic aneurysm repair ScienceDirect: S0741521411018568
Scandinavian Journal of Trauma, Resuscitation and Emergency Medicine High levels of soluble VEGF receptor 1 early after trauma are associated with shock, sympathoadrenal activation, glycocalyx degradation and inflammation in severely injured patients: a prospective study Sjtrem:1757-7241
Journal of Thrombosis and Haemostasis High sCD40L levels early after trauma are associated with enhanced shock, sympathoadrenal activation, tissue and endothelial damage, coagulopathy and mortality Wiley: source
British Journal of Dermatology Evaluation of autologous plasma skin test in patients with chronic idiopathic urticaria PubMed: 21910697
Arteriosclerosis, Thrombosis, and Vascular Biology. Diet Modulates Endogenous Thrombin Generation, A Biological Estimate of Thrombosis Risk, Independently of the Metabolic Status PubMed: 22859493
Journal of Vascular Surgery Medium-term effect of endovascular and open abdominal aortic aneurysm repair on thrombin generation and fibrinolysis PubMed: 23140799
PLoS ONE Markers of Thrombogenesis and Fibrinolysis and Their Relation to Inflammation and Endothelial Activation in Patients with Idiopathic Pulmonary Arterial Hypertension Plosone: Source
Journal of Vascular Surgery Effect of endovascular and open abdominal aortic aneurysm repair on thrombin generation and fibrinolysis Pubmed: 23140799
Haemetology Clinical impact of factor V Leiden, prothrombin G20210A, and MTHFR C677T mutations among sickle cell disease patients of Central India Pubmed: 23992124
Cogent Medicine Study the association of adiponectin with inflammation and hypercoagulability in case of type 2 diabetic subjects with renal dysfunction Tandfonline:Source
Interact Cardiovasc Thorac Surg. An ex vivo evaluation of blood coagulation and thromboresistance of two extracorporeal circuit coatings with reduced and full heparin dose Pubmed:24632424
Perfusion Ex vivo simulation of cardiopulmonary bypass with human blood for hemocompatibility testing PubMed: 26243277
J Thromb Haemost Comparison of the Effect of Dabigatran and Dalteparin on Thrombus Stability in a Murine Model of Venous Thromboembolism PubMed: 26514101
Acta Oncologica Turcica Thrombin activatable fibrinolysis inhibitor (TAFI), tissue factor pathway inhibitor (TFPI), and prothrombin fragment 1+2 levels in patients with advanced colorectal cancer aot:AOT_49_1_6_12.pdf
Advances in Medical Sciences Perioperative thrombocytopenia predicts poor outcome in patients undergoing transcatheter aortic valve implantation pubmed:29145170
Thrombosis Research The causes of thrombocytopenia after transcatheter aortic valve implantation pubmed:28582640
Acta Haematologica Elevations of Thrombotic Biomarkers in Hemoglobin H Disease Pubmed:29402840
Journal of Neurotrauma The cerebral thrombin system is activated after intracerebral hemorrhage and contributes to secondary lesion growth and poor neurological outcome in C57Bl/6 mice Pubmed: 31830857
Indian Journal of Public Health Research & Development Enhanced Fibrin-Lysis in Grade-1 Dengue Haemorrhagic Fever
Rheumatol Int The impact of anti-endothelial cell antibodies (AECAs) on the development of blood vessel damage in patients with systemic lupus erythematosus: the preliminary … Pubmed:35284968
Catalog No. Related products for research use of Rattus norvegicus (Rat) Organism species Applications (RESEARCH USE ONLY!)
RPA710Ra01 Recombinant Prothrombin Fragment 1+2 (F1+2) Positive Control; Immunogen; SDS-PAGE; WB.
PAA710Ra01 Polyclonal Antibody to Prothrombin Fragment 1+2 (F1+2) WB; IHC; ICC; IP.
LAA710Ra81 FITC-Linked Polyclonal Antibody to Prothrombin Fragment 1+2 (F1+2) WB; IHC; ICC; IF.
LAA710Ra71 Biotin-Linked Polyclonal Antibody to Prothrombin Fragment 1+2 (F1+2) WB; IHC; ICC.
SEA710Ra ELISA Kit for Prothrombin Fragment 1+2 (F1+2) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA710Ra CLIA Kit for Prothrombin Fragment 1+2 (F1+2) Chemiluminescent immunoassay for Antigen Detection.
LMA710Ra Multiplex Assay Kit for Prothrombin Fragment 1+2 (F1+2) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
KSA710Ra01 ELISA Kit DIY Materials for Prothrombin Fragment 1+2 (F1+2) Main materials for "Do It (ELISA Kit) Yourself".