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ELISA Kit DIY Materials for Ferritin (FE)

  • Product No.KSA518Hu01
  • Organism SpeciesHomo sapiens (Human) Same name, Different species.
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range0.156-10ng/mL
  • Applicable Sensitivity0.068ng/mL
  • ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
  • DownloadInstruction Manual
  • UOM 96T*596T*10 96T*20 96T*50 96T*100
  • FOB US$ 716  US$ 1194  US$ 2149  US$ 4179  US$ 7164 
    For more details, please contact local distributors!

SPECIFITY

The Abs in the kit have high sensitivity and excellent specificity for detection of Ferritin (FE). No significant cross-reactivity or interference between Ferritin (FE) and analogues was observed.

USAGE

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

STORAGE

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Magazine Citations
Molecular Endocrinology PGC-1α regulates hepatic hepcidin expression and iron homeostasis in response to inflammation. Pubmed: 23438894
experimental oncology Redox-regulation of gelatinases during growth of cisplatin-sensitive and resistant Guerin carcinoma. PubMed: 25804229
J Cancer Sci Ther Host H68D Genotype Affects Tumor Growth in Mouse Melanoma Open-Access: 1948-5956-1000353
Food Funct The effects of polysaccharides from the root of Angelica sinensis on tumor growth and iron metabolism in H22-bearing mice Pubmed:26757699
Physiological research The Labile Iron Pool in Monocytes Reflects the Activity of the Atherosclerotic Process in Men with Chronic Cardiovascular Disease pubmed:27782743
Expert Review of Hematology 4 (th) Mediterranean Multidisciplinary Course on Iron Anemia April 29 (th)-30 (th) 2016, Madrid, Spain. pubmed:27615544
Oxidative Medicine and Cellular Longevity Acidic Polysaccharide from Angelica sinensis Reverses Anemia of Chronic Disease Involving the Suppression of Inflammatory Hepcidin and NF-κB Activation pubmed:29147463
European Journal of Pharmaceutical Sciences sucrosomial® iron absorption studied by in vitro and ex-vivo models pubmed:29055735
Journal of Food Science and Technology In vitro assessment of bio-augmented minerals from peanut oil cakes fermented by Aspergillus oryzae through Caco-2 cells. pubmed:29051659
International Journal of Cuban Health & Medicine Anemia and Iron Deficiency Related to Inflammation, Helicobacter pylori Infection and Adiposity in Reproductive-age Cuban Women 10.1590/MEDICC.2017.1902030004 
Oman Medical Journal The Role of Insulin Therapy in Correcting Hepcidin Levels in Patients with Type 2 Diabetes Mellitus. pubmed:28584599
Food Bioscience Preparation of lactose-iron complex and its cyto-toxicity, in-vitro digestion and bioaccessibility in Caco-2 cell model system 10.1016/j.fbio.2017.10.001
Nutrients. Long-Term Dexamethasone Exposure Down-Regulates Hepatic TFR1 and Reduces Liver Iron Concentration in Rats pubmed:28629118
Food Chemistry Physicochemical characterization of mineral (iron/zinc) bound caseinate and their mineral uptake in Caco-2 cells Pubmed:29622185
Animal Feed Science and Technology Super-dosing phytase improves the growth performance of weaner pigs fed a low iron diet 10.1016:j.anifeedsci.2018.01.012
American Journal of Hematology Iron homeostasis in pregnancy and spontaneous abortion Pubmed: 30394565
The Nigerian postgraduate medical journal the official publication of the National Postgraduate Medical College of Nigeria Assessment of iron deficiency anaemia and its risk factors among adults with chronic kidney disease in a tertiary hospital in Nigeria
Haematologica New thiazolidinones reduce iron overload in mouse models of hereditary hemochromatosis and β-thalassemia Pubmed: 30792208
博士论文 PHYSIOLOGICAL ADAPTATIONS TO HEAT ACCLIMATION; REPERCUSSIONS ON CYCLING PERFORMANCE
Synthesis, characterization and cellular mineral absorption of nanoemulsions of Rhododendron arboreum flower extracts stabilized with gum arabic
Cell Research Transferrin plays a central role in coagulation balance by interacting with clotting factors Pubmed: 31811276
international journal of biological macromolecules Gum arabic capped copper nanoparticles: Synthesis, characterization, and applications Pubmed: 31904465
journal of the american college of nutrition Improvement of Mineral Absorption and Nutritional Properties of Citrullus vulgaris Seeds Using Solid-State Fermentation Pubmed: 32255407
LWT-FOOD SCIENCE AND TECHNOLOGY Whey protein-iron or zinc complexation decreases pro-oxidant activity of iron and increases iron and zinc bioavailability
J Mol Cell Cardiol Systemic iron deficiency does not affect the cardiac iron content and progression of heart failure 34139233
European Journal of Pharmacology The mechanism of the imbalance between proliferation and ferroptosis in pulmonary artery smooth muscle cells based on the activation of SLC7A11 Pubmed:35700835
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
NPA518Hu01 Native Ferritin (FE) Positive Control; Immunogen; SDS-PAGE; WB.
PAA518Hu01 Polyclonal Antibody to Ferritin (FE) WB; IHC; ICC; IP.
LAA518Hu71 Biotin-Linked Polyclonal Antibody to Ferritin (FE) WB; IHC; ICC.
MAA518Hu21 Monoclonal Antibody to Ferritin (FE) WB; IHC; ICC; IP.
SEA518Hu ELISA Kit for Ferritin (FE) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA518Hu CLIA Kit for Ferritin (FE) Chemiluminescent immunoassay for Antigen Detection.
LMA518Hu Multiplex Assay Kit for Ferritin (FE) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
KSA518Hu01 ELISA Kit DIY Materials for Ferritin (FE) Main materials for "Do It (ELISA Kit) Yourself".