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ELISA Kit DIY Materials for Triiodothyronine (T3)

3,3',5-Triiodo-L-Thyronine; Liothyronine; Cytomel; Tertroxin

  • Product No.KSA453Ge11
  • Organism SpeciesPan-species (General) Same name, Different species.
  • Reagent Contents Capture Antibody, Biotin-labeled Competitor, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
  • Applicable PrincipleCompetitive Inhibition ELISA for Antigen Detection
  • Detectable Range123.5-10,000pg/mL
  • Applicable Sensitivity51.7pg/mL
  • ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
  • DownloadInstruction Manual
  • UOM 96T*596T*10 96T*20 96T*50 96T*100
  • FOB US$ 979  US$ 1632  US$ 2938  US$ 5712  US$ 9792 
    For more details, please contact local distributors!

SPECIFITY

The Abs in the kit have high sensitivity and excellent specificity for detection of Triiodothyronine (T3). No significant cross-reactivity or interference between Triiodothyronine (T3) and analogues was observed.

USAGE

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

STORAGE

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Magazine Citations
Aquatic Toxicology Bioconcentration and metabolism of decabromodiphenyl ether (BDE-209) result in thyroid endocrine disruption in zebrafish larvae ScienceDirect: S0166445X12000215
Aquatic Toxicology Exposure of zebrafish embryos/larvae to TDCPP alters concentrations of thyroid hormones and transcriptions of genes involved in the hypothalamic–pituitary–thyroid axis PubMed: 23220413
European Journal of Zoological Research Effect of dietary tryptophan on plasma growth hormone and thyroid hormone in broiler chicks Scholarsresearchlibrary: Source
Life Science Journal Can Nigella Sativa oil (NSO) reverse hypothyroid status induced by PTU in rat? biochemical and histological studies Lifesciencesite: Source
PLoS One A Comparative Genotoxicity Study of a Supraphysiological Dose of Triiodothyronine (T3) in Obese Rats Subjected to Either Calorie-Restricted Diet or Hyperthyroidism Pubmed: 23468891
Aquat Toxicol Thyroid endocrine system disruption by pentachlorophenol: an in vitro and in vivo assay. ScienceDirect: S0166445X13002129
Aquatic Toxicology Impact of co-exposure with lead and decabromodiphenyl ether (BDE-209) on thyroid function in zebrafish larvae Pubmed:25456233
Chemosphere Exposure to2,2′,4,4′-tetrabromodiphenyl ether (BDE-47) alters thyroid hormone levels and thyroid hormone-reCavia (Guinea pig )lated gene transcription in manila clam Ruditapes philippinarum science:S0045653516302016
Toxicological Sciences Exposure of pregnant mice to perfluorobutanesulfonate causes hypothyroxinemia and developmental abnormalities in female offspring pubmed:27803384
Sci Rep Exposure of pregnant mice to triclosan impairs placental development and nutrient transport. pubmed:28322267
Aquat Toxicol. Developmental toxicity and thyroid hormone-disrupting effects of 2,4-dichloro-6-nitrophenol in Chinese rare minnow (Gobiocypris rarus). pubmed:28187359
Bulletin of the World Health Organization Endemic goitre in the Sudan despite longstanding programmes for the control of iodine deficiency disorders handle:123456789
Biological Rhythm Research Age-related and seasonal variations in plasma uncarboxylated osteocalcin in male Murrah buffaloes 09291016.2016.1275393
Toxicological Sciences Exposure of Pregnant Mice to Triclosan Causes Insulin Resistance via Thyroxine Reduction pubmed:28973666
Environmental Pollution Transgenerational endocrine disruption and neurotoxicity in zebrafish larvae after parental exposure to binary mixtures of decabromodiphenyl ether (BDE-209) and lead 10.1016/j.envpol.2017.06.053
Sciencepaper online 四溴双酚A对菲律宾蛤仔的生长抑制效应机理Growth inhibition Effects of TBBPA on juvenile manila clam Ruditapes philippinarum 5790267088906950000
Tropical Journal of Pharmaceutical Research Neuroprotective effects of thymoquinone against cerebellar histopathological changes in propylthiouracil-induced hypothyroidism in adult rats 10.4314/tjpr.v16i5.9
s-space Screening thyroid hormone disrupting effects of benzophenones using GH3, FRTL-5 cells and zebrafish 10371/137708
s-space Thyroid disrupting effects and associated mechanisms of TCPP in GH3 cell line and zebrafish (Danio rerio) larva and adult 10371/137708
Environmental Pollution Time-dependent inhibitory effects of Tris(1, 3-dichloro-2-propyl) phosphate on growth and transcription of genes involved in the GH/IGF axis, but not the HPT axis, in female zebrafish. pubmed:28624628
Environmental Science & Technology Thyroid hormone-disrupting potentials of major benzophenones in two cell lines (GH3 and FRTL-5) and embryo-larval zebrafish Pubmed:29995391
Environmental Pollution Prenatal exposure to polychlorinated biphenyl and umbilical cord hormones and birth outcomes in an island population Pubmed:29525625
Toxicological Sciences Impact of Perfluorooctane Sulfonate on Reproductive Ability of Female Mice through Suppression of Estrogen Receptor a-Activated Kisspeptin Neurons 10.1093:toxsci
Frontiers in Molecular Neuroscience Impact of Triclosan on Female Reproduction through Reducing Thyroid Hormones to Suppress Hypothalamic Kisspeptin Neurons in Mice Pubmed:29403355
Drug metabolism and disposition Activation of 5-HT1A receptors in the hypothalamic paraventricular nuclei (PVN) negatively regulates cytochrome P450 expression and activity in rat liver Pubmed:29555828
Archives Animal Breeding Age-related changes in testicular parameters and their relationship to thyroid hormones and testosterone in male Murrah buffaloes 10.5194:aab-61-191-2018
Indian Journal of Animal Sciences Endurance exercise causes adverse changes in some hematological and physio-biochemical indices in ponies under high altitude stress condition :
Environmental Pollution Transgenerational thyroid endocrine disruption induced by bisphenol S affects the early development of zebrafish offspring Pubmed: 30243188
Cell Reports Zika Virus Infection in Hypothalamus Causes Hormone Deficiencies and Leads to Irreversible Growth Delay and Memory Impairment in Mice Pubmed: 30404008
Drug Metabolism and Disposition Serotonin receptors of 5-HT2 type in the hypothalamic arcuate nuclei (ARC) positively regulate liver cytochrome P450 via stimulation of the growth hormone-releasing … Pubmed: 30518657
Science of the Total Environment Parental exposure to 6: 2 chlorinated polyfluorinated ether sulfonate (F53B) induced transgenerational thyroid hormone disruption in zebrafish Doi: 10.1016/j.scitotenv.2019.02.198
Theriogenology  Thyroid hormone profiles and TSH evaluation during early pregnancy and the transition period in dairy cows Pubmed: 30784791
Chemosphere Inhibition of growth in juvenile manila clam Ruditapes philippinarum: Potential adverse outcome pathway of TBBPA Pubmed: 30844590
Aquatic Toxicology Effects of cis-bifenthrin enantiomers on the growth, behavioral, biomarkers of oxidative damage and bioaccumulation in Xenopus laevis Pubmed: 31276910
ecotoxicology and environmental safety Exposure to humidifier disinfectants induces developmental effects and disrupts thyroid endocrine systems in zebrafish larvae Pubmed: 31539807
Cells MicroRNA-99a is a Potential Target for Regulating Hypothalamic Synaptic Plasticity in the Peri/Postmenopausal Depression Model Pubmed: 31540304
Chemosphere Exposure of adult mice to perfluorobutanesulfonate impacts ovarian functions through hypothyroxinemia leading to down-regulation of Akt-mTOR signaling Pubmed: 31809938
Autoimmunity Therapeutic effect of mesenchymal stem cell on Hashimoto's thyroiditis in a rat model by modulating Th17/Treg cell balance Pubmed: 31793369
AQUATIC TOXICOLOGY Interaction between hypoxia and perfluorobutane sulfonate on developmental toxicity and endocrine disruption in marine medaka embryos Pubmed: 32172180
Chemosphere Effect of triadimefon and its metabolite on adult amphibians Xenopus laevis Pubmed: 31743868
Nature Feeding induces cholesterol biosynthesis via the mTORC1–USP20–HMGCR axis Pubmed: 33177714
Toxicol Lett Sex-specific effects of triphenyltin chloride (TPT) on thyroid disruption and metabolizing enzymes in adult zebrafish (Danio rerio) Pubmed: 32525014
Ecotoxicology Changes in thyroid hormone levels and related gene expressions in embryo–larval zebraish exposed to binary combinations of bifenthrin and acetochlor Pubmed: 32468518
Brazilian Journal of Development Maternal exposure of triclosan causes fetal development restriction and female reproductive alterations in rat offspring/A exposição materna ao triclosan causa …
CHEMOSPHERE Effects of 2-ethylhexyl-4-methoxycinnamate (EHMC) on thyroid hormones and genes associated with thyroid, neurotoxic, and nephrotoxic responses in adult and larval zebrafish (Danio rerio)
Andrologia Thyroid hormones, Sertoli cell proliferation and differentiation in progenies from carbamazepine©\treated rat dams during pregnancy and lactation 33433934
The Journal of Clinical Endocrinology & Metabolism High levels of thyroid hormone impair regulatory T cells function via reduced PD-1 expression 33758937
Endocrinology Genetic Manipulation on Zebrafish duox Recapitulate the Clinical Manifestations of Congenital Hypothyroidism 34019632
Ecotoxicol Environ Saf Xenopus laevis tadpoles exposed to metamifop: Changes in growth, behavioral endpoints, neurotransmitters, antioxidant system and thyroid development 34126306
Journal of Hazardous Materials Exposure of adult zebrafish (Danio rerio) to Tetrabromobisphenol A causes neurotoxicity in larval offspring, an adverse transgenerational effect 33647619
Pharmaceutics Improved Therapeutic Efficiency against Obesity through Transdermal Drug Delivery Using Microneedle Arrays
Pharmaceutics The Selective NMDA Receptor GluN2B Subunit Antagonist CP-101,606 with Antidepressant Properties Modulates Cytochrome P450 Expression in the Liver 34683936
Aquat Toxicol Thyroid disruption and growth inhibition of zebrafish embryos/larvae by phenanthrene treatment at environmentally relevant concentrations 34933138
Environmental Pollution Probiotics inhibit the stunted growth defect of perfluorobutanesulfonate via stress and thyroid axes in zebrafish larvae 34428700
Ecotoxicol Environ Saf Perfluorohexanoic acid caused disruption of the hypothalamus-pituitary-thyroid axis in zebrafish larvae Pubmed:35131581
Toxics Environmental Co-Exposure to Potassium Perchlorate and Cd Caused Toxicity and Thyroid Endocrine Disruption in Zebrafish Embryos and Larvae (Danio rerio) Pubmed:35448459
Catalog No. Related products for research use of Pan-species (General) Organism species Applications (RESEARCH USE ONLY!)
CPA453Ge11 BSA Conjugated Triiodothyronine (T3) Immunogen; SDS-PAGE; WB.
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PAA453Ge01 Polyclonal Antibody to Triiodothyronine (T3) ELISA, CLIA. / IHC-Fr, ICC, IP (predicted).
LAA453Ge71 Biotin-Linked Polyclonal Antibody to Triiodothyronine (T3) WB; IHC; ICC.
MAA453Ge21 Monoclonal Antibody to Triiodothyronine (T3) WB; IHC; ICC; IP.
MAA453Ge23 Monoclonal Antibody to Triiodothyronine (T3) ELISA, CLIA. / IHC-Fr, ICC, IP (predicted).
MAA453Ge22 Monoclonal Antibody to Triiodothyronine (T3) ELISA, CLIA. / IHC-Fr, ICC, IP (predicted).
MAA453Ge24 Monoclonal Antibody to Triiodothyronine (T3) ELISA, CLIA. / IHC-Fr, ICC, IP (predicted).
MAA453Ge25 Monoclonal Antibody to Triiodothyronine (T3) ELISA, CLIA. / IHC-Fr, ICC, IP (predicted).
MAA453Ge27 Monoclonal Antibody to Triiodothyronine (T3) WB; IHC; ICC; IP.
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LMA453Ge Multiplex Assay Kit for Triiodothyronine (T3) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
KSA453Ge11 ELISA Kit DIY Materials for Triiodothyronine (T3) Main materials for "Do It (ELISA Kit) Yourself".