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ELISA Kit DIY Materials for Thyroxine (T4)
3,5,3',5'-Tetraiodothyronine; L-Thyroxine; Levothyroxine
- Product No.KSA452Ge11
- Organism SpeciesPan-species (General) Same name, Different species.
- Reagent Contents Capture Antibody, Biotin-labeled Competitor, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
- Detectable Sampleserum, plasma and other biological fluids
- Applicable PrincipleCompetitive Inhibition ELISA for Antigen Detection
- Detectable Range3.70-300ng/mL
- Applicable Sensitivity1.42ng/mL
- ApplicationsMain materials for "Do It(ELISA Kit) Yourself"
- Downloadn/a
- UOM 96T*596T*10 96T*20 96T*50 96T*100
- FOB
US$ 979
US$ 1632
US$ 2938
US$ 5712
US$ 9792
For more details, please contact local distributors!
SPECIFITY
The Abs in the kit have high sensitivity and excellent specificity for detection of Thyroxine (T4). No significant cross-reactivity or interference between Thyroxine (T4) and analogues was observed.
USAGE
1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
STORAGE
Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.
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| Magazine | Citations |
| Aquatic Toxicology | Bioconcentration and metabolism of decabromodiphenyl ether (BDE-209) result in thyroid endocrine disruption in zebrafish larvae ScienceDirect: S0166445X12000215 |
| Aquatic Toxicology | Exposure of zebrafish embryos/larvae to TDCPP alters concentrations of thyroid hormones and transcriptions of genes involved in the hypothalamic–pituitary–thyroid axis PubMed: 23220413 |
| Life Science Journal | Can Nigella Sativa oil (NSO) reverse hypothyroid status induced by PTU in rat? biochemical and histological studies Lifesciencesite: Source |
| Aquat Toxicol | Thyroid endocrine system disruption by pentachlorophenol: an in vitro and in vivo assay. ScienceDirect: S0166445X13002129 |
| Aquatic Toxicology | Impact of co-exposure with lead and decabromodiphenyl ether (BDE-209) on thyroid function in zebrafish larvae Pubmed:25456233 |
| Chemosphere | Exposure to2,2′,4,4′-tetrabromodiphenyl ether (BDE-47) alters thyroid hormone levels and thyroid hormone-reCavia (Guinea pig )lated gene transcription in manila clam Ruditapes philippinarum science:S0045653516302016 |
| Frontiers in cellular neuroscience | Elevated Thyroid Peroxidase Antibody Increases Risk of Post-partum Depression by Decreasing Prefrontal Cortex BDNF and 5-HT Levels in Mice pubmed:28119573 |
| Toxicological Sciences | Exposure of pregnant mice to perfluorobutanesulfonate causes hypothyroxinemia and developmental abnormalities in female offspring pubmed:27803384 |
| Sci Rep | Exposure of pregnant mice to triclosan impairs placental development and nutrient transport. pubmed:28322267 |
| Aquat Toxicol. | Developmental toxicity and thyroid hormone-disrupting effects of 2,4-dichloro-6-nitrophenol in Chinese rare minnow (Gobiocypris rarus). pubmed:28187359 |
| Bulletin of the World Health Organization | Endemic goitre in the Sudan despite longstanding programmes for the control of iodine deficiency disorders handle:123456789 |
| Biological Rhythm Research | Age-related and seasonal variations in plasma uncarboxylated osteocalcin in male Murrah buffaloes 09291016.2016.1275393 |
| Toxicological Sciences | Exposure of Pregnant Mice to Triclosan Causes Insulin Resistance via Thyroxine Reduction pubmed:28973666 |
| Environmental Pollution | Transgenerational endocrine disruption and neurotoxicity in zebrafish larvae after parental exposure to binary mixtures of decabromodiphenyl ether (BDE-209) and lead 10.1016/j.envpol.2017.06.053 |
| Sciencepaper online | 四溴双酚A对菲律宾蛤仔的生长抑制效应机理Growth inhibition Effects of TBBPA on juvenile manila clam Ruditapes philippinarum 5790267088906950000 |
| Tropical Journal of Pharmaceutical Research | Neuroprotective effects of thymoquinone against cerebellar histopathological changes in propylthiouracil-induced hypothyroidism in adult rats 10.4314/tjpr.v16i5.9 |
| s-space | Thyroid disrupting effects and associated mechanisms of TCPP in GH3 cell line and zebrafish (Danio rerio) larva and adult 10371/137708 |
| Environmental Pollution | Time-dependent inhibitory effects of Tris(1, 3-dichloro-2-propyl) phosphate on growth and transcription of genes involved in the GH/IGF axis, but not the HPT axis, in female zebrafish. pubmed:28624628 |
| Environmental pollution | Stereoselective degradation and thyroid endocrine disruption of lambda-cyhalothrin in lizards (Eremias argus) following oral exposure pubmed:28970022 |
| Environmental Science & Technology | Thyroid hormone-disrupting potentials of major benzophenones in two cell lines (GH3 and FRTL-5) and embryo-larval zebrafish Pubmed:29995391 |
| Environmental Pollution | Prenatal exposure to polychlorinated biphenyl and umbilical cord hormones and birth outcomes in an island population Pubmed:29525625 |
| Toxicological Sciences | Impact of Perfluorooctane Sulfonate on Reproductive Ability of Female Mice through Suppression of Estrogen Receptor a-Activated Kisspeptin Neurons 10.1093:toxsci |
| Frontiers in Molecular Neuroscience | Impact of Triclosan on Female Reproduction through Reducing Thyroid Hormones to Suppress Hypothalamic Kisspeptin Neurons in Mice Pubmed:29403355 |
| Drug metabolism and disposition | Activation of 5-HT1A receptors in the hypothalamic paraventricular nuclei (PVN) negatively regulates cytochrome P450 expression and activity in rat liver Pubmed:29555828 |
| International Journal of Endocrinology | Histopathological Characterization and Whole Exome Sequencing of Ectopic Thyroid: Fetal Architecture in a Functional Ectopic Gland from Adult Patient Pubmed:29593791 |
| Archives Animal Breeding | Age-related changes in testicular parameters and their relationship to thyroid hormones and testosterone in male Murrah buffaloes 10.5194:aab-61-191-2018 |
| Environmental Pollution | Transgenerational thyroid endocrine disruption induced by bisphenol S affects the early development of zebrafish offspring Pubmed: 30243188 |
| Journal of Agricultural and Food Chemistry | Metabolism of diflubenzuron in lizard (Eremias argus) and comparative toxicity of diflubenzuron and its metabolite Pubmed: 30346759 |
| Journal of Veterinary Research | Elastographic and morphological testicular changes in hypothyroidism–an experimental study Doi: 10.2478/jvetres-2018-0049 |
| Science of The Total Environment | Dose-dependent effects of flufenoxuron on thyroid system of mature female lizards (Eremias argus) and their offspring Pubmed: 30448662 |
| Cell Reports | Zika Virus Infection in Hypothalamus Causes Hormone Deficiencies and Leads to Irreversible Growth Delay and Memory Impairment in Mice Pubmed: 30404008 |
| Drug Metabolism and Disposition | Serotonin receptors of 5-HT2 type in the hypothalamic arcuate nuclei (ARC) positively regulate liver cytochrome P450 via stimulation of the growth hormone-releasing … Pubmed: 30518657 |
| Science of the Total Environment | Parental exposure to 6: 2 chlorinated polyfluorinated ether sulfonate (F53B) induced transgenerational thyroid hormone disruption in zebrafish Doi: 10.1016/j.scitotenv.2019.02.198 |
| Chemosphere | Inhibition of growth in juvenile manila clam Ruditapes philippinarum: Potential adverse outcome pathway of TBBPA Pubmed: 30844590 |
| Journal of Veterinary Research | Pituitary-testicular axis dysfunction in methimazole-induced hypothyroidism in rats Doi: 10.2478/jvetres-2019-0008 |
| Journal of Diabetes Research | The Adverse Effects of Thyrotropin Absence on Pancreatic β Cell Function in Mice |
| Aquatic Toxicology | Effects of cis-bifenthrin enantiomers on the growth, behavioral, biomarkers of oxidative damage and bioaccumulation in Xenopus laevis Pubmed: 31276910 |
| ecotoxicology and environmental safety | Exposure to humidifier disinfectants induces developmental effects and disrupts thyroid endocrine systems in zebrafish larvae Pubmed: 31539807 |
| Chemosphere | Exposure of adult mice to perfluorobutanesulfonate impacts ovarian functions through hypothyroxinemia leading to down-regulation of Akt-mTOR signaling Pubmed: 31809938 |
| Autoimmunity | Therapeutic effect of mesenchymal stem cell on Hashimoto's thyroiditis in a rat model by modulating Th17/Treg cell balance Pubmed: 31793369 |
| AQUATIC TOXICOLOGY | Interaction between hypoxia and perfluorobutane sulfonate on developmental toxicity and endocrine disruption in marine medaka embryos Pubmed: 32172180 |
| Clinical Immunology | The role of protein disulphide-isomerase A3 as autoantigen in the pathogenesis of autoimmune thyroiditis and related brain damage in adult mice Pubmed: 31982645 |
| Chemosphere | Effect of triadimefon and its metabolite on adult amphibians Xenopus laevis Pubmed: 31743868 |
| Nature | Feeding induces cholesterol biosynthesis via the mTORC1–USP20–HMGCR axis Pubmed: 33177714 |
| Toxicol Lett | Sex-specific effects of triphenyltin chloride (TPT) on thyroid disruption and metabolizing enzymes in adult zebrafish (Danio rerio) Pubmed: 32525014 |
| Ecotoxicology | Changes in thyroid hormone levels and related gene expressions in embryo–larval zebraish exposed to binary combinations of bifenthrin and acetochlor Pubmed: 32468518 |
| Int Immunopharmacol | Experimental evidence for alpha enolase as one potential autoantigen in the pathogenesis of both autoimmune thyroiditis and its related encephalopathy Pubmed: 32442899 |
| BioMed Research International | Pathophysiological Changes in Female Rats with Estrous Cycle Disorder Induced by Long-Term Heat Stress Pubmed: 32685488 |
| Brazilian Journal of Development | Maternal exposure of triclosan causes fetal development restriction and female reproductive alterations in rat offspring/A exposição materna ao triclosan causa … |
| CHEMOSPHERE | Effects of 2-ethylhexyl-4-methoxycinnamate (EHMC) on thyroid hormones and genes associated with thyroid, neurotoxic, and nephrotoxic responses in adult and larval zebrafish (Danio rerio) |
| Andrologia | Thyroid hormones, Sertoli cell proliferation and differentiation in progenies from carbamazepine©\treated rat dams during pregnancy and lactation 33433934 |
| The Journal of Clinical Endocrinology & Metabolism | High levels of thyroid hormone impair regulatory T cells function via reduced PD-1 expression 33758937 |
| Endocrinology | Genetic Manipulation on Zebrafish duox Recapitulate the Clinical Manifestations of Congenital Hypothyroidism 34019632 |
| Ecotoxicol Environ Saf | Xenopus laevis tadpoles exposed to metamifop: Changes in growth, behavioral endpoints, neurotransmitters, antioxidant system and thyroid development 34126306 |
| Clin Epigenetics | Genome?wide profiling of DNA methylation and gene expression unravel the epigenetic landscape in diabetes-related hypothyroidism 34092241 |
| Journal of Hazardous Materials | Exposure of adult zebrafish (Danio rerio) to Tetrabromobisphenol A causes neurotoxicity in larval offspring, an adverse transgenerational effect 33647619 |
| Pharmaceutics | The Selective NMDA Receptor GluN2B Subunit Antagonist CP-101,606 with Antidepressant Properties Modulates Cytochrome P450 Expression in the Liver 34683936 |
| Aquat Toxicol | Thyroid disruption and growth inhibition of zebrafish embryos/larvae by phenanthrene treatment at environmentally relevant concentrations 34933138 |
| Ecotoxicol Environ Saf | Perfluorohexanoic acid caused disruption of the hypothalamus-pituitary-thyroid axis in zebrafish larvae Pubmed:35131581 |
| Toxics | Environmental Co-Exposure to Potassium Perchlorate and Cd Caused Toxicity and Thyroid Endocrine Disruption in Zebrafish Embryos and Larvae (Danio rerio) Pubmed:35448459 |
| Fluorene-9-Bisphenol Induced Thyroid-Disrupting Effects and Histopathological Alterations in Adult Zebrafish (Danio Rerio) |
| Catalog No. | Related products for research use of Pan-species (General) Organism species | Applications (RESEARCH USE ONLY!) |
| CPA452Ge11 | BSA Conjugated Thyroxine (T4) | Immunogen; SDS-PAGE; WB. |
| CPA452Ge21 | OVA Conjugated Thyroxine (T4) | Immunogen; SDS-PAGE; WB. |
| PAA452Ge01 | Polyclonal Antibody to Thyroxine (T4) | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
| LAA452Ge81 | FITC-Linked Polyclonal Antibody to Thyroxine (T4) | WB; IHC; ICC; IF. |
| MAA452Ge28 | Monoclonal Antibody to Thyroxine (T4) | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
| MAA452Ge25 | Monoclonal Antibody to Thyroxine (T4) | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
| MAA452Ge23 | Monoclonal Antibody to Thyroxine (T4) | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
| MAA452Ge22 | Monoclonal Antibody to Thyroxine (T4) | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
| MAA452Ge21 | Monoclonal Antibody to Thyroxine (T4) | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
| CEA452Ge | ELISA Kit for Thyroxine (T4) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| IEA452Ge | Instant ELISA Kit for Thyroxine (T4) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| MEA452Ge | Mini Samples ELISA Kit for Thyroxine (T4) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| CCA452Ge | CLIA Kit for Thyroxine (T4) | Chemiluminescent immunoassay for Antigen Detection. |
| LMA452Ge | Multiplex Assay Kit for Thyroxine (T4) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |
| KSA452Ge11 | ELISA Kit DIY Materials for Thyroxine (T4) | Main materials for "Do It(ELISA Kit) Yourself" |