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ELISA Kit DIY Materials for Protein Kinase C Epsilon (PKCe)

PKC-E; PRKCE; PRKC-E; NPKC-Epsilon

  • Product No.KSA439Hu01
  • Organism SpeciesHomo sapiens (Human) Same name, Different species.
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleTissue homogenates, cell lysates, cell culture supernates and other biological fluids.
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range1.56-100ng/mL
  • Applicable Sensitivity0.54ng/mL
  • ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
  • Downloadn/a
  • UOM 96T*596T*10 96T*20 96T*50 96T*100
  • FOB US$ 1134  US$ 1890  US$ 3402  US$ 6615  US$ 11340 
    For more details, please contact local distributors!

SPECIFITY

The Abs in the kit have high sensitivity and excellent specificity for detection of Protein Kinase C Epsilon (PKCe). No significant cross-reactivity or interference between Protein Kinase C Epsilon (PKCe) and analogues was observed.

USAGE

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

STORAGE

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Magazine Citations
Am J Physiol Lung Cell Mol Physiol. Aging causes a slowing in ciliary beat frequency, mediated by PKCε. Pubmed:24487393
Cell Stress & Chaperones Effect of single and repeated heat stress on chemical signals of heat shock response cascade in the rat's heart. pubmed:29178005
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
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KSA439Hu01 ELISA Kit DIY Materials for Protein Kinase C Epsilon (PKCe) Main materials for "Do It (ELISA Kit) Yourself".