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ELISA Kit DIY Materials for Estrogen Receptor Beta (ERb)
ER-B; ESRB; ESTRB; NR3A2; NR3-A2; ESR2; Estrogen Receptor 2; Nuclear Receptor Subfamily 3,Group A,Member 2
- Product No.KSA437Hu01
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
- Detectable SampleSerum, plasma, tissue homogenates, cell lysates and other biological fluids.
- Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
- Detectable Range0.312-20ng/mL
- Applicable Sensitivity0.117ng/mL
- ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
- Downloadn/a
- UOM 96T*596T*10 96T*20 96T*50 96T*100
- FOB
US$ 1134
US$ 1890
US$ 3402
US$ 6615
US$ 11340
For more details, please contact local distributors!
SPECIFITY
The Abs in the kit have high sensitivity and excellent specificity for detection of Estrogen Receptor Beta (ERb). No significant cross-reactivity or interference between Estrogen Receptor Beta (ERb) and analogues was observed.
USAGE
1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
STORAGE
Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.
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| Magazine | Citations |
| The Journal of Steroid Biochemistry and Molecular Biology | Neuroprotective action of raloxifene against hypoxia-induced damage in mouse hippocampal cells depends on ERα but not ERβ or GPR30 signalling Pubmed:24846829 |
| Biochem Biophys Res Commun | Differential expression of estrogen receptor α and β isoforms in multiple and solitary leiomyomas PubMed: 26529545 |
| Mol Neurobiol | Selective Aryl Hydrocarbon Receptor Modulator 3,3′-Diindolylmethane Impairs AhR and ARNT Signaling and Protects Mouse Neuronal Cells Against Hypoxia PubMed: 26476840 |
| J Steroid Biochem Mol Biol | Neuroprotective action of raloxifene against hypoxia-induced damage in mouse hippocampal cells depends on ERα but not ERβ or GPR31 signalling PubMed: 24846830 |
| Journal of Environmental Sciences | Bisphenol A exposure alters release of immune and developmental modulators and expression of estrogen receptors in human fetal lung fibroblasts science:S1001074216300924 |
| Biol Open. | Involvement of BDNF/TrkB and ERK/CREB axes in nitroglycerin-induced rat migraine and effects of estrogen on these signals in the migraine. pubmed:27875242 |
| Biomedicine & Pharmacotherapy | Postmenopausal osteoporosis is associated with the regulation of SP, CGRP, VIP, and NPY Pubmed:29807224 |
| Journal of Applied Pharmaceutical Science | Physical Signs of Illness, Liver Functions and Reproductive Parameters of Female Rats Supplemented with Melaleuca cajuputi Methanolic Extract Pbulication:325682424 |
| Carleton University Research Virtual Environment | In Vitro Study of Mechanisms Underlying the Developmental Effects of Bisphenol A Using Human Fetal Lung Fibroblasts |
| Life Sciences | MiR-145 regulates steroidogenesis in mouse primary granulosa cells through targeting Crkl 34273377 |
| Cardiometry | Modifying effect of obesity on the content of sex hormones and their receptors in endometrial adenocarcinoma and its surrounding tissue |