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ELISA Kit DIY Materials for Alanine Aminotransferase (ALT)

GPT; ALAT; SGPT; AAT1; GPT1; Serum Glutamic Pyruvic Transaminase; Alanine Transaminase; Glutamate pyruvate transaminase 1; Glutamic--alanine transaminase 1

  • Product No.KSA207Mu01
  • Organism SpeciesMus musculus (Mouse) Same name, Different species.
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleSerum, plasma, tissue homogenates, cell lysates and other biological fluids.
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range0.156-10ng/mL
  • Applicable Sensitivity0.064ng/mL
  • ApplicationsMain materials for "Do It(ELISA Kit) Yourself"
  • Downloadn/a
  • UOM 96T*596T*10 96T*20 96T*50 96T*100
  • FOB US$ 1166  US$ 1944  US$ 3499  US$ 6804  US$ 11664 
    For more details, please contact local distributors!

SPECIFITY

The Abs in the kit have high sensitivity and excellent specificity for detection of Alanine Aminotransferase (ALT). No significant cross-reactivity or interference between Alanine Aminotransferase (ALT) and analogues was observed.

USAGE

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

STORAGE

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Magazine Citations
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Proceedings of the National Academy of Sciences Pathogenesis of emerging severe fever with thrombocytopenia syndrome virus in C57/BL6 mouse model PubMed: PMC3382536
African Journal of Pharmacy and Pharmacology Protective effect of Panax ginseng against N-acetyl-p-aminophenol-induced hepatotoxicity in rats Academicjournals: Source
Kidney International Adrenocorticotropic hormone ameliorates acute kidney injury by steroidogenic-dependent and-independent mechanisms Pubmed: 23325074
European Journal of Pharmacology Protective effect of Et-1 receptor antagonist bosentan on paracetamol induced acute liver toxicity in rats ScienceDirect: S0014299914000235
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Experimental and Molecular Pathology Resolvin E1 and chemerin C15 peptide do not improve rodent non-alcoholic steatohepatitis Pubmed:25681756
Gastroenterol Res Pract Assessing the Effect of Leptin on Liver Damage in Case of Hepatic Injury Associated with Paracetamol Poisoning PubMed: 26697061
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Oncotarget Dihydromyricetin protects against liver ischemia/reperfusion induced apoptosis via activation ofFOXO3a-mediated autophagy. pubmed:27793014
Scientific Reports Adiponectin protects the rats liver against chronic intermittent hypoxia induced injury throughAMP-activated protein kinase pathway. pubmed:27678302
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International Immunopharmacology Dimethyl fumarate ameliorates autoimmune hepatitis in mice by blocking NLRP3 inflammasome activation Pubmed:35605433
Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
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KSA207Mu01 ELISA Kit DIY Materials for Alanine Aminotransferase (ALT) Main materials for "Do It(ELISA Kit) Yourself"