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ELISA Kit DIY Materials for Creatine Kinase, Muscle (CKM)

CK-MM; CKMM; CK3; CK-M; MM-CK; M-CK; Creatine kinase M-type, N-terminally processed

  • Product No.KSA109Ra01
  • Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range1.56-100ng/mL
  • Applicable Sensitivity0.62ng/mL
  • ApplicationsMain materials for "Do It(ELISA Kit) Yourself"
  • Downloadn/a
  • UOM 96T*596T*10 96T*20 96T*50 96T*100
  • FOB US$ 1231  US$ 2052  US$ 3694  US$ 7182  US$ 12312 
    For more details, please contact local distributors!

SPECIFITY

The Abs in the kit have high sensitivity and excellent specificity for detection of Creatine Kinase, Muscle (CKM). No significant cross-reactivity or interference between Creatine Kinase, Muscle (CKM) and analogues was observed.

USAGE

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

STORAGE

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Magazine Citations
Journal of Inflammation Assessment of endothelium and inflammatory response at the onset of reperfusion injury in hand surgery PubMed: 22583529
PLoS One Extraocular Muscle Characteristics Related to Myasthenia Gravis Susceptibility PubMed: PMC3568149
Kidney Int. Fructokinase activity mediates dehydration-induced renal injury Pubmed: 24336030
Methylxanthines and cardiotoxicity Methylxanthine induced cardiotoxicity and its mechanisms: An experimental study 230b:34bf146150b42971d76dda5e8329bc5f2f0b.pdf
Molecular Medicine Reports Protective effects of N (2)‑L‑alanyl‑L‑glutamine mediated by the JAK2/STAT3 signaling pathway on myocardial ischemia reperfusion Pubmed:29393473
Toxicological Sciences Proteomic and Metabolomic Profiling Identify Plasma Biomarkers for Exposure to Ultra-low Levels of Carfentanil Pubmed: 30321427
Clinical & Translational Oncology The effects of human sera conditioned by high-intensity exercise sessions and training on the tumorigenic potential of cancer cells Pubmed: 32447643
Catalog No. Related products for research use of Rattus norvegicus (Rat) Organism species Applications (RESEARCH USE ONLY!)
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KSA109Ra01 ELISA Kit DIY Materials for Creatine Kinase, Muscle (CKM) Main materials for "Do It(ELISA Kit) Yourself"