ELISA Kit DIY Materials for Leptin (LEP)

OB; OBS; Obesity Homolog; Obesity Factor; Obese Protein

  • Product No.KSA084Mu01
  • Organism SpeciesMus musculus (Mouse) Same name, Different species.
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable Sampleserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range0.156-10ng/mL
  • Applicable Sensitivity0.056ng/mL.
  • ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
  • Downloadn/a
  • UOM 96T*596T*10 96T*20 96T*50 96T*100
  • FOB US$ 842  US$ 1404  US$ 2527  US$ 4914  US$ 8424 
    For more details, please contact local distributors!

SPECIFITY

The Abs in the kit have high sensitivity and excellent specificity for detection of Leptin (LEP).No significant cross-reactivity or interference between Leptin (LEP) and analogues was observed.

USAGE

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

STORAGE

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Magazine Citations
European Journal of Pharmacology Puerarin improves insulin resistance and modulates adipokine expression in rats fed a high-fat diet PubMed: 20869961
Indian Journal of Pharmacology Effect of Argyreia speciosa root extract on cafeteria diet-induced obesity in rats Ijp: source
Journal of endocrinology Testosterone protects from metabolic syndrome-associated prostate inflammation: an experimental study in rabbit PubMed: 22010203
PLoS One. Multi-Functional Roles of Chitosan as a Potential Protective Agent against Obesity PubMed: 23342013
Molecular and Cellular Endocrinology Soy isoflavones modulate adipokines and myokines to regulate lipid metabolism in adipose tissue, skeletal muscle and liver of male Huanjiang mini-pigs ScienceDirect: S0303720712004194
Journal of Animal and Feed Sciences Ovarian, metabolic and endocrine indexes in dairy cows with different body condition scores Ifzz: Source
British Journal of Nutrition n-6: n-3 PUFA ratio is involved in regulating lipid metabolism and inflammation in pigs Pubmed: 23947577
Int J Mol Sci. Maternal high folic acid supplement promotes glucose intolerance and insulin resistance in male mouse offspring fed a high-fat diet. Pubmed: 24736781
Endocr J. CTRP3 modulates the expression and secretion of adipokines in 3T3-L1 adipocytes Pubmed:25168658
Animal Physiology EFFECT OF LEPTIN GENE ON SERUM LEPTIN CONCENTRATION AND BEEF QUALITY IN CZECH FLECKVIEH BULLS Mendelu:Source
Evid Based Complement Alternat Med. Tinospora crispa Ameliorates Insulin Resistance Induced by High Fat Diet in Wistar Rats Pubmed:Pmc4363645
general endocrinology Oxytocin reverse ovariectomy-induced Osteoper and body fat gain Pubmed:24506069
Respiratory Physiology & Neurobiology Maternal high-fat diet feeding during pregnancy and lactation augments lung inflammation and remodeling in the offspring Pubmed:25500158
Sci Rep Hepatic steatosis depresses alpha-1-antitrypsin levels in human and rat acute pancreatitis PubMed: 26634430
Evid Based Complement Alternat Med Tinospora crispa PubMed: 25821506
Ankara Effects of breeder age and early energy restriction on fattening performance, some meat quality traits and plasma leptin concentration of broilers Dergiler: 11
MEDICINA VETERIN Comparative measurements of the morphometric pattern and energy profile of Crioula mares in the final third of gestation, with different body condition scores Scielo: S0102-09352015000300707
Pancreatic Disorders & Therapy Pancreatic Exocrine Insufficiency Affects Not Only Digestibility of Nutrients and Growth, but Also Body Composition and Endocrinological Parameters – Study on Piglets Used As a Model for Children Journals: 2165-7092-%20S5-00
Veterinary Word Effect of extended photoperiod during winter on growth and onset of puberty in Murrah buffalo heifers Pubmed:27051212
Animal Anterior pituitary influence on adipokine expression and secretion by porcine adipocytes. Pubmed:27074715
ANNALS OF ANIMAL SCIENCE Comparison of the effect of a standard inclusion level of inorganic zinc to organic form at lowered level on bone development in growing male Ross broiler chickens journalprintahead:87
Annals of Animal Science Comparison of the Effect of a Standard Inclusion Level of Inorganic Zinc to Organic Form at Lowered Level on Bone Development in Growing Male Ross Broiler Chickens view:87
Obes Surg. Changes on Adipose Tissue Distribution After Laparoscopic Roux-en-Y Gastric Bypass in Obese Göttingen Minipig. Effects on Glucose Metabolism. Pubmed:27185175
Acta Histochemica Favorable outcomes of hydroxychloroquine in insulin resistance may be accomplished by adjustment of the endothelial dysfunction as well as the skewed balance of adipokines science:S0065128116301234
J Biomed Mater Res A Nanosized titanium dioxide resulted in the activation of TGF-β/Smads/p38MAPK pathway in renal inflammation and fibration of mice Pubmed:26850371
Clin Epigenetics. The bromodomain inhibitor N-methyl pyrrolidone reduced fat accumulation in an ovariectomized rat model Pubmed:27110299
International Journal of Molecular Sciences Oral Supplementation with a Special Additive of Retinyl Palmitate and Alpha Tocopherol Reduces Growth Retardation in Young Pancreatic Duct Ligated Pigs Used as a Model for Children Suffering from Exocrine Pancreatic Insufficiency pubmed:27690005
Journal of Animal Science Changes in small intestinal morphometry and electrogenic nutrient transport in young pigs with pancreatic exocrine insufficiency publications:jas
International Journal of Endocrinology The Changes of Serum Leptin and Kisspeptin Levels in Chinese Children and Adolescents in Different Pubertal Stages pubmed:27990162
journal of animal science The effect of GnRH vaccination on performance, carcass, and meat quality and hormonal regulation in boars, barrows, and gilts. pubmed:27482668
journal of animal science Diet-induced modifications to milk composition have long-term effects on offspring growth in rabbits. pubmed:28380600
Evidence-Based Complementary and Alternative Medicine Four-Week Consumption of Malaysian Honey Reduces Excess Weight Gainand Improves Obesity-Related Parameters in High Fat Diet Induced ObeseRats. pubmed:28246535
Asian-Australasian Journal of Animal Sciences Dietary Niacin Supplementation Suppressed Hepatic Lipid Accumulation in Rabbits pubmed:27004817
Stem Cell Research & Therapy Serial changes in the proliferation and differentiation of adipose-derived stem cells after ionizingradiation. pubmed:27530249
Obesity Surgery Changes on Adipose Tissue Distribution After Laparoscopic Roux-en-Y Gastric Bypass in ObeseGöttingen Minipig. Effects on Glucose Metabolism. pubmed:27185175
Meat Sci Estimation of the intramuscular fat content of m. longissimus thoracis in crossbred beef cattle based on live animal measurements. pubmed:27940421
THE INDIAN JOURNAL OF VETERINARY SCIENCES AND BIOTECHNOLOGY Correlation of Leptin, Insulin and Glucose During Late Gestation and Early Lactation in Murrah Buffaloes 7086
Journal of Animal and Plant Sciences Effects Of Maca (Lepıdıum Meyenıı Walp) Powder On Serum Indıces And Metabolıc Responses In Racehorses 57cf073008ae83b37462323a.pdf
Biological Trace Element Research Effect of Dietary Phytase Supplementation on Bone and Hyaline Cartilage Development of Broilers Fed with Organically Complexed Copper in a Cu-Deficient Diet article:10.1007
Revista Brasileira de Ciência Avícola Effect of Zinc Level and Source (Zinc Oxide Vs. Zinc Glycine) on Bone Mechanical and Geometric Parameters, and Histomorphology in Male Ross 308 Broiler Chicken 10.159:1806-9061-2016-0285
Applied Animal Behaviour Science Evaluating measures of exploratory behaviour in sows around farrowing and during lactation− A pilot study 10.1016/j.jacl.2017.03.007
Proceedings of the National Academy of Sciences of the United States of America (PNAS) cAMP-inducible coactivator CRTC3 attenuates brown adipose tissue thermogenesis Pubmed:29784793
Oxidative Medicine and Cellular Longevity Changes of Plasma FABP4, CRP, Leptin, and Chemerin Levels in relation to Different Dietary Patterns and Duodenal-Jejunal Omega Switch Surgery in Sprague … Pubmed:29849871
Frontiers in Endocrinology Circulating and Adipose Tissue mRNA Levels of Zinc-α2-Glycoprotein, Leptin, High-Molecular-Weight Adiponectin, and Tumor Necrosis Factor-Alpha in … Pubmed:29755407
Journal of Agricultural and Food Chemistry Metabolomics for the effect of biotin and nicotinamide on transition dairy cows Pubmed:29758980
Frontiers in Physiology Ileal Transposition Surgery Decreases Fat Mass and Improves Glucose Metabolism in Diabetic GK Rats: Possible Involvement of FGF21 Pubmed:29593555
Bioscience Reports Effect of maternal hypothyroidism during pregnancy on insulin resistance, lipid accumulation and mitochondrial dysfunction in skeletal muscle of fetal rats Pubmed:29784871
Neurochemical Research The leptin, dopamine and serotonin receptors in hypothalamic POMC-neurons of normal and obese rodents Pubmed:29397535
PLoS One Subsequent somatic axis and bone tissue metabolism responses to a low-zinc diet with or without phytase inclusion in broiler chickens Pubmed:29373588
Biochemical and Biophysical Research Communications Chronic cold exposure results in subcutaneous adipose tissue browning and altered global metabolism in Qinghai-Tibetan plateau pika (Ochotona curzoniae) Pubmed:29626477
Alcohol and Alcoholism Leptin Expression and Gene Methylation Patterns in Alcohol-Dependent Patients with Ethyltoxic Cirrhosis—Normalization After Liver Transplantation and Implications … Pubmed:29912265
Iranian Red Crescent Medical Journal  The Effect of Aerobic Training on Tumor Growth, Adiponectin, Leptin and Ghrelin in Mice with Breast Cancer ircmj-20-03-13305.pdf
Pakistan Journal of Zoology Effects of Intrauterine Growth Retardation on Growth, Meat Quality and Muscle Fiber Composition of Pigs. :
Oncotarget Epigallocatechin-3-gallate suppresses differentiation of adipocytes via regulating the phosphorylation of FOXO1 mediated by PI3K-AKT signaling in 3T3-L1 … Pubmed:29484120
УСПЕХИ ГЕРОНТОЛОГИИ ВЛИЯНИЕ МЕТФОРМИНА НА МЕТАБОЛИЧЕСКИЕ ПОКАЗАТЕЛИ И ГИПОТАЛАМИЧЕСКИЕ СИГНАЛЬНЫЕ СИСТЕМЫ У КРЫС С ОЖИРЕНИЕМ … AG_2018-31-01.pdf
Food Science and Technology Research Combinational Effect of Pine Needle Polysaccharide and Kudzu Flavonoids on Cell Differentiation and Fat Metabolism in 3T3-L1 Cells Doi: 10.3136/fstr.24.903
Nutrients Effects of Metformin Combined with Lactoferrin on Lipid Accumulation and Metabolism in Mice Fed with High-Fat Diet Pubmed: 30400147
Psychopharmacology Betahistine effects on weight-related measures in patients treated with antipsychotic medications: a double-blind placebo-controlled study Pubmed: 30382354
Progress in Nutrition Effect of fermented food consumption on biochemical parameters and adipokines levels Doi: 0.23751/pn.v20i4.7374
THE INDIAN JOURNAL OF VETERINARY SCIENCES & BIOTECHNOLOGY THE INDIAN JOURNAL OF VETERINARY SCIENCES & BIOTECHNOLOGY
Biomedicine & Pharmacotherapy Raspberry ketone and Garcinia Cambogia rebalanced disrupted insulin resistance and leptin signaling in rats fed high fat fructose diet Pubmed: 30530230
Nutrition in the Prevention and Treatment of Abdominal Obesity Effects of Diet-Induced Early-Stage Obesity on a Low-Testosterone Gottingen Minipig
Scientific Reports Obesity Aggravates Acute Pancreatitis via Damaging Intestinal Mucosal Barrier and Changing Microbiota Composition in Rats Pubmed: 30635594
Atherosclerotic diseases Cardiotrophin-1 and leptin as cardiovascular risk markers in male patients with obstructive sleep apnea syndrome Pubmed: 30775602
PLoS One Impact of exposure to diesel exhaust during pregnancy on mammary gland development and milk composition in the rabbit Pubmed: 30763367
Scientific Journal of Kurdistan University of Medical Sciences Effect of High Intensity Interval Training (HIIT) on visceral and subcutaneous levels of leptin and plasma insulin and glucose in male Wistar rats
PLOS ONE The evidence of metabolic-improving effect of metformin in Ay/a mice with genetically-induced melanocortin obesity and the contribution of hypothalamic … Pubmed: 30870482
International Journal of Pharmaceutical Research & Allied Sciences Osteoprotegerin Levels among Saudi Cardiovascular Patients.
Journal of Ethnopharmacology Anti-obesity effect of argel (Solenostemma argel) on obese rats fed a high fat diet
Gene Expression of adipokines and their receptors in adipose tissue of women with class 3 obesity with or without hypertension Pubmed: 30940525
中国药理学通报 冷暴露对高脂饮食小鼠脂肪棕色化的影响
Arch Physiol Biochem Associations of apelin, leptin, irisin, ghrelin, insulin, glucose levels, and lipid parameters with physical activity during eight weeks of regular exercise training Pubmed: 31290696
Food & Function A dietary polysaccharide from Eucheuma cottonii downregulates proinflammatory cytokines and ameliorates osteoarthritis-associated cartilage degradation in obese …
Nutrients High-dose Glycerol Monolaurate Up-Regulated Beneficial Indigenous Microbiota without Inducing Metabolic Dysfunction and Systemic Inflammation: New Insights …
Developmental Dynamics Puberty is a critical window for the impact of diet on mammary gland development in the rabbit Pubmed: 31348557
Adv Gerontol The Effect of Different Types of Bariatric Surgery on Metabolic and Hormonal Parameters in Rats with a Decompensated Form of Type II Diabetes Mellitus Pubmed: 31228372
Proceedings of the Royal Society B: Biological Sciences Paternal chronic folate supplementation induced the transgenerational inheritance of acquired developmental and metabolic changes in chickens Pubmed: 31506054
Food & Function Collaborative Effects of Chlorogenic Acid and Caffeine on Lipid Metabolism via AMPKα-LXRα/SREBP-1c Pathway in High-Fat Diet-Induced Obese Mice Pubmed: 31670366
journal of ethnopharmacology Dietary supplementation of Morus nigra L. leaves decrease fat mass partially through elevating leptin-stimulated lipolysis in pig model Pubmed: 31756448
Annals of Nutrition and Metabolism Serum Asprosin Concentrations Are Increased and Associated with Insulin Resistance in Children with Obesity Pubmed: 31775140
Journal of Ethnopharmacology Upregulation of MC4R and PPAR-α expression mediates the anti-obesity activity of Moringa oleifera Lam. in high-fat diet-induced obesity in rats Pubmed: 31911179
Journal of Natural Products Prenylated Phenolic Compounds from the Aerial Parts of Glycyrrhiza uralensis as PTP1B and α-Glucosidase Inhibitors Pubmed: 32196343
gynecological endocrinology Salivary Leptin and Chemerin; a novel way of gestational diabetes screening Pubmed: 32274942
Frontiers in Endocrinology RFRP-3, the Mammalian Ortholog of GnIH, Is a Novel Modulator Involved in Food Intake and Glucose Homeostasis Pubmed: 32328034
Ann Nutr Metab Copeptin and Nesfatin-1 Are Interrelated Biomarkers with Roles in the Pathogenesis of Insulin Resistance in Chinese Children with Obesity Pubmed: 33027789
ANDROLOGIA The effect of metformin treatment on the basal and gonadotropin‐stimulated steroidogenesis in male rats with type 2 diabetes mellitus Pubmed: 32951228
GENERAL AND COMPARATIVE ENDOCRINOLOGY Adipokines expression profiles in both plasma and peri renal adipose tissue in Large White and Meishan sows: a possible involvement in the fattening and … Pubmed: 32827511
Nutrition & Diabetes C-Peptide and leptin system in dichorionic, small and appropriate for gestational age twins—possible link to metabolic programming? Pubmed: 32778645
BIOLOGICAL TRACE ELEMENT RESEARCH Ameliorative Effect of Ananas comosus on Cobalt Chloride-Induced Hypoxia in Caco2 cells via HIF-1α, GLUT 1, VEGF, ANG and FGF Pubmed: 32654099
plos one Serum leptin level is associated with phase angle in CKD5 patients not undergoing dialysis Pubmed: 30089154
Journal of reproduction and fertility Blastomere removal affects homeostatic control leading to obesity in male mice Pubmed: 33112292
Journal of Evolutionary Biochemistry and Physiology The Effect of Diet-Induced and Melanocortin Obesity on Expression of Tryptophan Hydroxylase 2 in the Dorsal Raphe Nucleus and Ventral Tegmental Area in Mice
Indian Journal of Animal Sciences Peripheral concentrations of metabolic and inflammatory indicators during transition period and their relationship with postpartum clinical endometritis in dairy …
СОСТОЯНИЕ МЕЛАТОНИН-АССОЦИИРОВАННОЙ ГОРМОНАЛЬНОЙ СИСТЕМЫ У ДЕТЕЙ С ИЗБЫТОЧНОЙ МАССОЙ ТЕЛА
biorxiv Altered hematopoietic system and self-tolerance in Bardet-Biedl Syndrome
The Egyptian Journal of Neurology, Psychiatry and Neurosurgery volume Serum leptin and body mass index in a sample of Egyptian multiple sclerosis patients
Biosci Rep A 12-week aerobic exercise improves metabolic function with lower adipose tissue and ectopic fat in high fat diet fed rats 33432988
Cell Metab TMBIM1 is an inhibitor of adipogenesis and its depletion promotes adipocyte hyperplasia and improves obesity-related metabolic disease 34107313
International Journal of Endocrinology Effect of Ileal Transposition (IT) on Angiopoietin-Like Protein-8 (ANGPTL8) and Pentraxin (PTX3) Plasma Level in Sprague-Dawley Rats Fed High-Fat Diet?¡­ 34035808
EUROPEAN JOURNAL OF LIPID SCIENCE AND TECHNOLOGY Different Ratios of Corn and Coconut Oil Blends in High©\Fat Diets Influence Fat Deposition without Altering Metabolic Biomarkers in Male Rats
The pharma innovation Journal Comparative study on genital status, energy balance and mineral content of swamp buffalo (Luit) heifers and cows under organized system of rearing in Assam?¡­
Indian Journal of Forensic Medicine & Toxicology Altered Serum Marker of Adipokines Profile in Breast Cancer Women
PPAR Research Activation of the Peroxisome Proliferator-Activated Receptors (PPAR-α/γ) and the Fatty Acid Metabolizing Enzyme Protein CPT1A by Camel Milk Treatment … 34306045
Br J Nutr The Association of Pre-Pregnancy BMI On Leptin, Ghrelin, Adiponectin, and IGF-1 In Breast Milk, A Case Control Study 34348809
Ann Med Surg (Lond) Maternal and fetal serum leptin levels and their association with maternal and fetal variables and labor: A cross-sectional study 34815864
Biofactors Vitamin D3 enhances the effects of omega‐3 oils against metabolic dysfunction‐associated fatty liver disease in rat 34767670
Nat Commun GP73 is a TBC-domain Rab GTPase-activating protein contributing to the pathogenesis of non-alcoholic fatty liver disease without obesity 34853313
Int J Mol Sci The Effects of Separate and Combined Treatment of Male Rats with Type 2 Diabetes with Metformin and Orthosteric and Allosteric Agonists of Luteinizing Hormone … Pubmed:35008624
Bull Exp Biol Med Comparative Study of the Restoring Effect of Metformin, Gonadotropin, and Allosteric Agonist of Luteinizing Hormone Receptor on Spermatogenesis in Male Rats with … Pubmed:35175480
Naunyn Schmiedebergs Arch Pharmacol Intragastric safflower yellow and its main component HSYA improve leptin sensitivity before body weight change in diet-induced obese mice Pubmed:35201390
npj Biofilms and Microbiomes The microbiota-gut-kidney axis mediates host osmoregulation in a small desert mammal Pubmed:35379849
Ecotoxicol Environ Saf Monochromatic blue light not green light exposure is associated with continuous light-induced hepatic steatosis in high fat diet fed-mice via oxidative stress Pubmed:35588616
Biomed Res Int Seahorse Protein Hydrolysate Ameliorates Proinflammatory Mediators and Cartilage Degradation on Posttraumatic Osteoarthritis with an Obesity Rat Model Pubmed:35509713
Endocr J A novel anti-obesity mechanism for liraglutide by improving adipose tissue leptin resistance in high-fat diet-fed obese mice Pubmed:35705299
Impact of pre-breeding feeding practices on rabbit mammary gland development at mid-pregnancy.
High Circulating Fibroblast Growth Factor-21 Levels as a Prognostic Marker in Fatty Pancreas Patients
Dairy Cattle Physiology Division ICAR-National Dairy Research Institute, Karnal-132001, Haryana, India Corresponding Authors Email ID: menalsh08vets …
Scientific Reports SARS-CoV2 Induced Biochemical Mechanisms in Liver Damage and Intestinal Lesions.
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