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ELISA Kit DIY Materials for Interleukin 15 (IL15)

  • Product No.KSA061Ra01
  • Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range15.6-1000pg/mL
  • Applicable Sensitivity6.0pg/mL
  • ApplicationsMain materials for "Do It(ELISA Kit) Yourself"
  • Downloadn/a
  • UOM 96T*596T*10 96T*20 96T*50 96T*100
  • FOB US$ 1231  US$ 2052  US$ 3694  US$ 7182  US$ 12312 
    For more details, please contact local distributors!

SPECIFITY

The Abs in the kit have high sensitivity and excellent specificity for detection of Interleukin 15 (IL15). No significant cross-reactivity or interference between Interleukin 15 (IL15) and analogues was observed.

USAGE

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

STORAGE

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Magazine Citations
Endocrine Expression of interleukin-15 and inflammatory cytokines in skeletal muscles of STZ-induced diabetic rats: effect of resistance exercise training Pubmed: 24006180
Endocrine Time course of IL-15 expression after acute resistance exercise in trained rats: effect of diabetes and skeletal muscle phenotype Pubmed:25522723
Med Sci Moni miR-15b is Downregulated in Myasthenia Gravis Patients and Directly Regulates the Expression of Interleukin-15 (IL-15) in Experimental Myasthenia Gravis Mice PubMed: 26087886
Exercise physiology Effects of intrinsic aerobic capacity, aging and physical activity on interleukin-15 protein level in serum and skeletal muscle Dspace: Handle
Biotechnology Letters Novel adjuvant for immunization against tuberculosis: DNA vaccine expressing Mycobacterium tuberculosis antigen 85A and interleukin-15 fusion product elicits strong immune responses in mice s10529-017-2342-1
Journal of Controlled Release Interleukin-15 and cisplatin co-encapsulated thermosensitive polypeptide hydrogels for combined immuno-chemotherapy. pubmed:28408199
Catalog No. Related products for research use of Rattus norvegicus (Rat) Organism species Applications (RESEARCH USE ONLY!)
RPA061Ra01 Recombinant Interleukin 15 (IL15) Positive Control; Immunogen; SDS-PAGE; WB.
PAA061Ra01 Polyclonal Antibody to Interleukin 15 (IL15) WB; IHC; ICC; IP.
MAA061Ra21 Monoclonal Antibody to Interleukin 15 (IL15) WB; IHC; ICC; IP.
MAA061Ra22 Monoclonal Antibody to Interleukin 15 (IL15) WB; IHC; ICC; IP.
LAA061Ra72 Biotin-Linked Monoclonal Antibody to Interleukin 15 (IL15) WB; IHC; ICC.
SEA061Ra ELISA Kit for Interleukin 15 (IL15) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA061Ra Multiplex Assay Kit for Interleukin 15 (IL15) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
PSA061Ra01 Antibody Pair for Interleukin 15 (IL15) ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays.
KSA061Ra01 ELISA Kit DIY Materials for Interleukin 15 (IL15) Main materials for "Do It(ELISA Kit) Yourself"